Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositis
Intestinal mucositis (IM) is a frequent adverse effect in anticancer therapy without standard treatment. The oil obtained from sucupira (Pterodon emarginatus) has anti-inflammatory properties, and the soybean lecithin reduces the intestinal toxicity of several xenobiotics. However, their water insol...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Toxicology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750022001603 |
| _version_ | 1811178765432127488 |
|---|---|
| author | Jeruza Ferraz Filgueiras Di Miceli Maria Emília Rabelo Andrade Paula Lopes Armond Carvalho Elandia Aparecida Santos Anna Eliza Maciel de Faria Mota Oliveira Caio Pinho Fernandes Rodrigo Alves Soares Cruz Rafael Garrett Vanessa Carla Furtado Mosqueira Geovanni Dantas Cassali Cecile D'Haese Bernard Nysten Jacqueline Isaura Alvarez Leite Valbert Nascimento Cardoso Raquel Silva Araújo |
| author_facet | Jeruza Ferraz Filgueiras Di Miceli Maria Emília Rabelo Andrade Paula Lopes Armond Carvalho Elandia Aparecida Santos Anna Eliza Maciel de Faria Mota Oliveira Caio Pinho Fernandes Rodrigo Alves Soares Cruz Rafael Garrett Vanessa Carla Furtado Mosqueira Geovanni Dantas Cassali Cecile D'Haese Bernard Nysten Jacqueline Isaura Alvarez Leite Valbert Nascimento Cardoso Raquel Silva Araújo |
| author_sort | Jeruza Ferraz Filgueiras Di Miceli |
| collection | DOAJ |
| description | Intestinal mucositis (IM) is a frequent adverse effect in anticancer therapy without standard treatment. The oil obtained from sucupira (Pterodon emarginatus) has anti-inflammatory properties, and the soybean lecithin reduces the intestinal toxicity of several xenobiotics. However, their water insolubility impairs the in vivo application. For this reason, we evaluated if the nanoencapsulation of sucupira oil (SO) in lecithin-based nanocapsules (SO-NC) could be a therapeutically effective system for the treatment of IM in murine cisplatin (CDDP)-induced intestinal mucositis model. SO was analyzed by LC-HRMS/MS and HPLC. SO-NC was prepared by nanoprecipitation and characterized using DLS, HPLC, and AFM. Mice body weight and food consumption were assessed daily during experimental mucositis induced by CDDP. The animals were euthanized, and intestinal permeability, inflammatory mediators, and intestinal histology were performed. SO-NC demonstrated adequate characteristics for oral administration as size under 300 nm, IP < 0.3, high EE, and spherical shape. In vitro cytotoxicity performed against RAW 264.7 cell lines resulted in cell viability above 80 % confirming the non-cytotoxic profile of SO (IC50 268 µg/mL) and SO-NC (IC50 118.5 µg/mL) up to 117.2 µg/mL. The untreated mice showed intestinal toxicity after i.p. of CDDP, principally weight loss, increased intestinal permeability, and MPO and TNF-α levels. Surprisingly, the administration of SO to CDDP-mucositis animals did not circumvent the CDDP effects and increased intestinal permeability. However, SO-NC proved efficient in mitigating the experimental intestinal mucositis by improving intestinal epithelium architecture, reducing intestinal permeability, and improving the MPO levels. In conclusion, SO-NC can positively impact intestinal mucositis by promoting mucosal recovery. This is a promising strategy for developing a new treatment for intestinal mucositis. |
| first_indexed | 2024-04-11T06:23:32Z |
| format | Article |
| id | doaj.art-f4268f0c0fa34f34aa86a3f04f303367 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-11T06:23:32Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-f4268f0c0fa34f34aa86a3f04f3033672022-12-22T04:40:28ZengElsevierToxicology Reports2214-75002022-01-01915371547Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositisJeruza Ferraz Filgueiras Di Miceli0Maria Emília Rabelo Andrade1Paula Lopes Armond Carvalho2Elandia Aparecida Santos3Anna Eliza Maciel de Faria Mota Oliveira4Caio Pinho Fernandes5Rodrigo Alves Soares Cruz6Rafael Garrett7Vanessa Carla Furtado Mosqueira8Geovanni Dantas Cassali9Cecile D'Haese10Bernard Nysten11Jacqueline Isaura Alvarez Leite12Valbert Nascimento Cardoso13Raquel Silva Araújo14Department of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, BrazilDepartment of Clinical and Toxicological Analysis, Pharmacy Faculty, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, BrazilDepartment of Clinical and Toxicological Analysis, Pharmacy Faculty, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, BrazilDepartment of Biochemistry and Immunology, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, BrazilDepartment of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, BrazilDepartment of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, BrazilDepartment of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, BrazilMetabolomics Laboratory, Institute of Chemistry, Universidade Federal do Rio de Janeiro, Av. Pedro Calmon, 550, Rio de Janeiro 21941-598, RJ, BrazilDepartment of Pharmacy – Pharmacy School, Universidade Federal de Ouro Preto, (UFOP), Morro do Cruzeiro, Ouro Preto 35400-000, MG, BrazilDepartment de General Pathology, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, BrazilUniversité Catholique de Louvain, Institute of Condensed Matter and Nanosciences, Bio & Soft Matter, 1348 Louvain-la-Neuve, BelgiumUniversité Catholique de Louvain, Institute of Condensed Matter and Nanosciences, Bio & Soft Matter, 1348 Louvain-la-Neuve, BelgiumDepartment of Biochemistry and Immunology, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, BrazilDepartment of Clinical and Toxicological Analysis, Pharmacy Faculty, Universidade Federal de Minas Gerais (UFMG), Av. Presidente Antônio Carlos 6627, Campus Pampulha, Belo Horizonte 31270-901, MG, BrazilDepartment of Biological and Health Sciences, Universidade Federal do Amapá (UNIFAP), Campus Marco Zero, Macapá 68903-419, AP, Brazil; Corresponding author.Intestinal mucositis (IM) is a frequent adverse effect in anticancer therapy without standard treatment. The oil obtained from sucupira (Pterodon emarginatus) has anti-inflammatory properties, and the soybean lecithin reduces the intestinal toxicity of several xenobiotics. However, their water insolubility impairs the in vivo application. For this reason, we evaluated if the nanoencapsulation of sucupira oil (SO) in lecithin-based nanocapsules (SO-NC) could be a therapeutically effective system for the treatment of IM in murine cisplatin (CDDP)-induced intestinal mucositis model. SO was analyzed by LC-HRMS/MS and HPLC. SO-NC was prepared by nanoprecipitation and characterized using DLS, HPLC, and AFM. Mice body weight and food consumption were assessed daily during experimental mucositis induced by CDDP. The animals were euthanized, and intestinal permeability, inflammatory mediators, and intestinal histology were performed. SO-NC demonstrated adequate characteristics for oral administration as size under 300 nm, IP < 0.3, high EE, and spherical shape. In vitro cytotoxicity performed against RAW 264.7 cell lines resulted in cell viability above 80 % confirming the non-cytotoxic profile of SO (IC50 268 µg/mL) and SO-NC (IC50 118.5 µg/mL) up to 117.2 µg/mL. The untreated mice showed intestinal toxicity after i.p. of CDDP, principally weight loss, increased intestinal permeability, and MPO and TNF-α levels. Surprisingly, the administration of SO to CDDP-mucositis animals did not circumvent the CDDP effects and increased intestinal permeability. However, SO-NC proved efficient in mitigating the experimental intestinal mucositis by improving intestinal epithelium architecture, reducing intestinal permeability, and improving the MPO levels. In conclusion, SO-NC can positively impact intestinal mucositis by promoting mucosal recovery. This is a promising strategy for developing a new treatment for intestinal mucositis.http://www.sciencedirect.com/science/article/pii/S2214750022001603NanocapsulesLecithinPterodon emarginatusIntestinal permeabilityMucositis |
| spellingShingle | Jeruza Ferraz Filgueiras Di Miceli Maria Emília Rabelo Andrade Paula Lopes Armond Carvalho Elandia Aparecida Santos Anna Eliza Maciel de Faria Mota Oliveira Caio Pinho Fernandes Rodrigo Alves Soares Cruz Rafael Garrett Vanessa Carla Furtado Mosqueira Geovanni Dantas Cassali Cecile D'Haese Bernard Nysten Jacqueline Isaura Alvarez Leite Valbert Nascimento Cardoso Raquel Silva Araújo Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositis Toxicology Reports Nanocapsules Lecithin Pterodon emarginatus Intestinal permeability Mucositis |
| title | Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositis |
| title_full | Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositis |
| title_fullStr | Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositis |
| title_full_unstemmed | Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositis |
| title_short | Lecithin-based nanocapsule loading sucupira (Pterodon emarginatus) oil effects in experimental mucositis |
| title_sort | lecithin based nanocapsule loading sucupira pterodon emarginatus oil effects in experimental mucositis |
| topic | Nanocapsules Lecithin Pterodon emarginatus Intestinal permeability Mucositis |
| url | http://www.sciencedirect.com/science/article/pii/S2214750022001603 |
| work_keys_str_mv | AT jeruzaferrazfilgueirasdimiceli lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT mariaemiliarabeloandrade lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT paulalopesarmondcarvalho lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT elandiaaparecidasantos lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT annaelizamacieldefariamotaoliveira lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT caiopinhofernandes lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT rodrigoalvessoarescruz lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT rafaelgarrett lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT vanessacarlafurtadomosqueira lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT geovannidantascassali lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT ceciledhaese lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT bernardnysten lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT jacquelineisauraalvarezleite lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT valbertnascimentocardoso lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis AT raquelsilvaaraujo lecithinbasednanocapsuleloadingsucupirapterodonemarginatusoileffectsinexperimentalmucositis |