Ambient Particulate Matter Induces Vascular Smooth Muscle Cell Phenotypic Changes via NOX1/ROS/NF-κB Dependent and Independent Pathways: Protective Effects of Polyphenols
Epidemiological studies have demonstrated an association between ambient particulate matter (PM) exposure and vascular diseases. Here, we observed that treatment with ambient PM increased cell migration ability in vascular smooth muscle cells (VSMCs) and pulmonary arterial SMCs (PASMCs). These resul...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/5/782 |
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| author | Chia-Chi Ho Yu-Cheng Chen Ming-Hsien Tsai Hui-Ti Tsai Chen-Yi Weng Shaw-Fang Yet Pinpin Lin |
| author_facet | Chia-Chi Ho Yu-Cheng Chen Ming-Hsien Tsai Hui-Ti Tsai Chen-Yi Weng Shaw-Fang Yet Pinpin Lin |
| author_sort | Chia-Chi Ho |
| collection | DOAJ |
| description | Epidemiological studies have demonstrated an association between ambient particulate matter (PM) exposure and vascular diseases. Here, we observed that treatment with ambient PM increased cell migration ability in vascular smooth muscle cells (VSMCs) and pulmonary arterial SMCs (PASMCs). These results suggest that VSMCs and PASMCs transitioned from a differentiated to a synthetic phenotype after PM exposure. Furthermore, treatment with PM increased intracellular reactive oxygen species (ROS), activated the NF-κB signaling pathway, and increased the expression of proinflammatory cytokines in VSMCs. Using specific inhibitors, we demonstrated that PM increased the migration ability of VSMCs via the nicotinamide–adenine dinucleotide phosphate (NADPH) oxidase 1 (NOX1)/ROS-dependent NF-κB signaling pathway, which also partially involved in the induction of proinflammatory cytokines. Finally, we investigated whether nature polyphenolic compounds prevent PM-induced migration and proinflammatory cytokines secretion in VSMCs. Curcumin, resveratrol, and gallic acid prevented PM<sub>2.5</sub>-induced migration via the ROS-dependent NF-κB signaling pathway. However, honokiol did not prevent PM<sub>2.5</sub>-induced migration or activation of the ROS-dependent NF-κB signaling pathway. On the other hand, all polyphenols prevented PM<sub>2.5</sub>-induced cytokines secretion. These data indicated that polyphenols prevented PM-induced migration and cytokine secretion via blocking the ROS-dependent NF-κB signaling pathway in VSMCs. However, other mechanisms may also contribute to PM-induced cytokine secretion. |
| first_indexed | 2024-03-10T11:24:26Z |
| format | Article |
| id | doaj.art-f4271221bbd64a1799ab4f6e05e7ab23 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T11:24:26Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f4271221bbd64a1799ab4f6e05e7ab232023-11-21T19:47:00ZengMDPI AGAntioxidants2076-39212021-05-0110578210.3390/antiox10050782Ambient Particulate Matter Induces Vascular Smooth Muscle Cell Phenotypic Changes via NOX1/ROS/NF-κB Dependent and Independent Pathways: Protective Effects of PolyphenolsChia-Chi Ho0Yu-Cheng Chen1Ming-Hsien Tsai2Hui-Ti Tsai3Chen-Yi Weng4Shaw-Fang Yet5Pinpin Lin6National Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan 53053, TaiwanNational Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan 53053, TaiwanNational Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan 53053, TaiwanNational Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan 53053, TaiwanNational Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan 53053, TaiwanInstitute of Cellular and System Medicine, National Health Research Institutes, Zhunan 53053, TaiwanNational Institute of Environmental Health Sciences, National Health Research Institutes, Zhunan 53053, TaiwanEpidemiological studies have demonstrated an association between ambient particulate matter (PM) exposure and vascular diseases. Here, we observed that treatment with ambient PM increased cell migration ability in vascular smooth muscle cells (VSMCs) and pulmonary arterial SMCs (PASMCs). These results suggest that VSMCs and PASMCs transitioned from a differentiated to a synthetic phenotype after PM exposure. Furthermore, treatment with PM increased intracellular reactive oxygen species (ROS), activated the NF-κB signaling pathway, and increased the expression of proinflammatory cytokines in VSMCs. Using specific inhibitors, we demonstrated that PM increased the migration ability of VSMCs via the nicotinamide–adenine dinucleotide phosphate (NADPH) oxidase 1 (NOX1)/ROS-dependent NF-κB signaling pathway, which also partially involved in the induction of proinflammatory cytokines. Finally, we investigated whether nature polyphenolic compounds prevent PM-induced migration and proinflammatory cytokines secretion in VSMCs. Curcumin, resveratrol, and gallic acid prevented PM<sub>2.5</sub>-induced migration via the ROS-dependent NF-κB signaling pathway. However, honokiol did not prevent PM<sub>2.5</sub>-induced migration or activation of the ROS-dependent NF-κB signaling pathway. On the other hand, all polyphenols prevented PM<sub>2.5</sub>-induced cytokines secretion. These data indicated that polyphenols prevented PM-induced migration and cytokine secretion via blocking the ROS-dependent NF-κB signaling pathway in VSMCs. However, other mechanisms may also contribute to PM-induced cytokine secretion.https://www.mdpi.com/2076-3921/10/5/782ambient particulate mattervascular phenotypic changesmigrationinflammationoxidative stressNADPH oxidase 1 |
| spellingShingle | Chia-Chi Ho Yu-Cheng Chen Ming-Hsien Tsai Hui-Ti Tsai Chen-Yi Weng Shaw-Fang Yet Pinpin Lin Ambient Particulate Matter Induces Vascular Smooth Muscle Cell Phenotypic Changes via NOX1/ROS/NF-κB Dependent and Independent Pathways: Protective Effects of Polyphenols Antioxidants ambient particulate matter vascular phenotypic changes migration inflammation oxidative stress NADPH oxidase 1 |
| title | Ambient Particulate Matter Induces Vascular Smooth Muscle Cell Phenotypic Changes via NOX1/ROS/NF-κB Dependent and Independent Pathways: Protective Effects of Polyphenols |
| title_full | Ambient Particulate Matter Induces Vascular Smooth Muscle Cell Phenotypic Changes via NOX1/ROS/NF-κB Dependent and Independent Pathways: Protective Effects of Polyphenols |
| title_fullStr | Ambient Particulate Matter Induces Vascular Smooth Muscle Cell Phenotypic Changes via NOX1/ROS/NF-κB Dependent and Independent Pathways: Protective Effects of Polyphenols |
| title_full_unstemmed | Ambient Particulate Matter Induces Vascular Smooth Muscle Cell Phenotypic Changes via NOX1/ROS/NF-κB Dependent and Independent Pathways: Protective Effects of Polyphenols |
| title_short | Ambient Particulate Matter Induces Vascular Smooth Muscle Cell Phenotypic Changes via NOX1/ROS/NF-κB Dependent and Independent Pathways: Protective Effects of Polyphenols |
| title_sort | ambient particulate matter induces vascular smooth muscle cell phenotypic changes via nox1 ros nf κb dependent and independent pathways protective effects of polyphenols |
| topic | ambient particulate matter vascular phenotypic changes migration inflammation oxidative stress NADPH oxidase 1 |
| url | https://www.mdpi.com/2076-3921/10/5/782 |
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