Biological characteristics of Paenibacillus polymyxa GBR-1 involved in root rot of stored Korean ginseng
Background: This study aims to describe the characterization of Paenibacillus polymyxa GBR-1 (GBR-1) with respect to its positive and negative effects on plants. Methods: The morphological characteristics of GBR-1 were identified with microscopy, and subjected to Biolog analysis for identification....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845315000962 |
| _version_ | 1818956555856379904 |
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| author | Young Soo Kim Balaraju Kotnala Young Ho Kim Yongho Jeon |
| author_facet | Young Soo Kim Balaraju Kotnala Young Ho Kim Yongho Jeon |
| author_sort | Young Soo Kim |
| collection | DOAJ |
| description | Background: This study aims to describe the characterization of Paenibacillus polymyxa GBR-1 (GBR-1) with respect to its positive and negative effects on plants.
Methods: The morphological characteristics of GBR-1 were identified with microscopy, and subjected to Biolog analysis for identification. Bacterial population and media optimization were determined by a growth curve. The potential for GBR-1 as a growth promoting agent, to have antagonistic activity, and to have hydrolytic activity at different temperatures was assessed. The coinoculation of GBR-1 with other microorganisms and its pathogenicity on various stored plants, including ginseng, were assessed.
Results: Colony morphology, endospore-bearing cells, and cell division of GBR-1 were identified by microscopy; identification was performed by utilizing the Biolog system, gas chromatography of fatty acid methyl esters (GC-FAME). GBR-1 showed the strongest antagonistic activity against fungal and bacterial pathogens. GBR-1 cell numbers were relatively higher when the cells were cultured in brain heart infusion (BHI) medium when compared with other media. Furthermore, the starch-hydrolytic activity was influenced by GBR-1 at higher temperature compared to low temperatures. GBR-1 was pathogenic to some of the storage plants. Coinoculation of GBR-1 with other pathogens causes differences in rotting on ginseng roots. A significant growth promotion was observed in tobacco seedlings treated with GBR-1 suspensions under in vitro conditions, suggesting that its volatile organic compounds (VOCs) might play a role in growth promotion.
Conclusion: The results of this study indicate that GBR-1 has both positive and negative effects on ginseng root and other stored plants as a potential biocontrol agent and eliciting in vitro growth promotion. |
| first_indexed | 2024-12-20T10:55:49Z |
| format | Article |
| id | doaj.art-f4277f34394441dbbf9f236cd8a776fb |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-12-20T10:55:49Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-f4277f34394441dbbf9f236cd8a776fb2022-12-21T19:43:09ZengElsevierJournal of Ginseng Research1226-84532016-10-0140445346110.1016/j.jgr.2015.09.003Biological characteristics of Paenibacillus polymyxa GBR-1 involved in root rot of stored Korean ginsengYoung Soo Kim0Balaraju Kotnala1Young Ho Kim2Yongho Jeon3Department of Bioresource Sciences, Andong National University, Andong, KoreaAgricultural Science and Technology Research Institute, Andong National University, Andong, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, KoreaDepartment of Bioresource Sciences, Andong National University, Andong, KoreaBackground: This study aims to describe the characterization of Paenibacillus polymyxa GBR-1 (GBR-1) with respect to its positive and negative effects on plants. Methods: The morphological characteristics of GBR-1 were identified with microscopy, and subjected to Biolog analysis for identification. Bacterial population and media optimization were determined by a growth curve. The potential for GBR-1 as a growth promoting agent, to have antagonistic activity, and to have hydrolytic activity at different temperatures was assessed. The coinoculation of GBR-1 with other microorganisms and its pathogenicity on various stored plants, including ginseng, were assessed. Results: Colony morphology, endospore-bearing cells, and cell division of GBR-1 were identified by microscopy; identification was performed by utilizing the Biolog system, gas chromatography of fatty acid methyl esters (GC-FAME). GBR-1 showed the strongest antagonistic activity against fungal and bacterial pathogens. GBR-1 cell numbers were relatively higher when the cells were cultured in brain heart infusion (BHI) medium when compared with other media. Furthermore, the starch-hydrolytic activity was influenced by GBR-1 at higher temperature compared to low temperatures. GBR-1 was pathogenic to some of the storage plants. Coinoculation of GBR-1 with other pathogens causes differences in rotting on ginseng roots. A significant growth promotion was observed in tobacco seedlings treated with GBR-1 suspensions under in vitro conditions, suggesting that its volatile organic compounds (VOCs) might play a role in growth promotion. Conclusion: The results of this study indicate that GBR-1 has both positive and negative effects on ginseng root and other stored plants as a potential biocontrol agent and eliciting in vitro growth promotion.http://www.sciencedirect.com/science/article/pii/S1226845315000962antagonistic activitybacterial morphologyginseng root rotgrowth promotionPaenibacillus polymyxa GBR-1 |
| spellingShingle | Young Soo Kim Balaraju Kotnala Young Ho Kim Yongho Jeon Biological characteristics of Paenibacillus polymyxa GBR-1 involved in root rot of stored Korean ginseng Journal of Ginseng Research antagonistic activity bacterial morphology ginseng root rot growth promotion Paenibacillus polymyxa GBR-1 |
| title | Biological characteristics of Paenibacillus polymyxa GBR-1 involved in root rot of stored Korean ginseng |
| title_full | Biological characteristics of Paenibacillus polymyxa GBR-1 involved in root rot of stored Korean ginseng |
| title_fullStr | Biological characteristics of Paenibacillus polymyxa GBR-1 involved in root rot of stored Korean ginseng |
| title_full_unstemmed | Biological characteristics of Paenibacillus polymyxa GBR-1 involved in root rot of stored Korean ginseng |
| title_short | Biological characteristics of Paenibacillus polymyxa GBR-1 involved in root rot of stored Korean ginseng |
| title_sort | biological characteristics of paenibacillus polymyxa gbr 1 involved in root rot of stored korean ginseng |
| topic | antagonistic activity bacterial morphology ginseng root rot growth promotion Paenibacillus polymyxa GBR-1 |
| url | http://www.sciencedirect.com/science/article/pii/S1226845315000962 |
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