The Value of Circulating Tumor Cells and Tumor Markers Detection in Lung Cancer Diagnosis
Objective Circulating tumor cells are complete tumor cells with multi-scale analysis values that present a high potential for lung cancer diagnosis. To enhance the accuracy of lung cancer diagnosis, we detected circulating tumor cells by the innovated conical micro filter integrated microfluidic sys...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-04-01
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| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/15330338231166754 |
| _version_ | 1827960413676896256 |
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| author | Sumin Guo MD Jingyu Chen BS Po Hu MD Chen Li MD Xiang Wang MD Ning Chen PhD Jiale Sun Yongfeng Wang BS Jianling Wang BS Weikuan Gu PhD Shucai Wu MD |
| author_facet | Sumin Guo MD Jingyu Chen BS Po Hu MD Chen Li MD Xiang Wang MD Ning Chen PhD Jiale Sun Yongfeng Wang BS Jianling Wang BS Weikuan Gu PhD Shucai Wu MD |
| author_sort | Sumin Guo MD |
| collection | DOAJ |
| description | Objective Circulating tumor cells are complete tumor cells with multi-scale analysis values that present a high potential for lung cancer diagnosis. To enhance the accuracy of lung cancer diagnosis, we detected circulating tumor cells by the innovated conical micro filter integrated microfluidic system. Methods We recruited 45 subjects of study, including 22 lung cancer patients, 2 precancerous patients, the control group including 14 healthy participants, and 7 patients with lung benign lesions in this prospective study. We calculated the area under the receiver operating characteristic curve of circulating tumor cells, cytokeratin19 fragment, carcinoma embryonic antigen, squamous cell carcinoma, neuron-specific enolase, and their combination, respectively, while compared the circulating tumor cells levels between vein blood and arterial blood. A conical shape filter embedded in a microfluidic chip was used to improve the detection capability of circulating tumor cells. Results The study indicated that the sensitivity, specificity, positive predictive value, and negative predictive value of circulating tumor cells detection were 81.8%, 90.5%, 90.0%, and 82.6%, respectively. The circulating tumor cells level of lung cancer patient was significantly higher than that of the control group ( P < .05). The area under the curve of circulating tumor cells, cytokeratin19 fragment, carcinoma embryonic antigen, squamous cell carcinoma, and neuron-specific enolase alone was 0.838, 0.760, 0.705, 0.614, and 0.636, respectively. The combination area under the curve of the 4 tumor markers (cytokeratin19 fragment, carcinoma embryonic antigen, squamous cell carcinoma, and neuron-specific enolase) was 0.805 less than that of circulating tumor cells alone. Together, the total area under the curve of circulating tumor cell and the 4 tumor markers were 0.847, showing the highest area under the curve value among all biomarkers. In addition, this study found that there was no significant difference in positive rate of circulating tumor cell between arterial and venous blood samples. Conclusion The circulating tumor cells detection technology by conical micro filter integrated microfluidic could be used for lung cancer diagnosis with high sensitivity and specificity. Complementary combination of circulating tumor cells and conventional 4 lung cancer markers could enhance the clinical application accuracy. Venous blood should be used as a routine sample for circulating tumor cells detections. |
| first_indexed | 2024-04-09T16:08:08Z |
| format | Article |
| id | doaj.art-f427c6f1853b422e93f81e0595667cd2 |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-04-09T16:08:08Z |
| publishDate | 2023-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-f427c6f1853b422e93f81e0595667cd22023-04-25T01:03:36ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382023-04-012210.1177/15330338231166754The Value of Circulating Tumor Cells and Tumor Markers Detection in Lung Cancer DiagnosisSumin Guo MD0Jingyu Chen BS1Po Hu MD2Chen Li MD3Xiang Wang MD4Ning Chen PhD5Jiale Sun6Yongfeng Wang BS7Jianling Wang BS8Weikuan Gu PhD9Shucai Wu MD10 Department of Internal Medicine, , Shijiazhuang, Hebei, People's Republic of China Department of Chinese Medicine Internal Medicine, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China Department of Oncology, Hebei Chest Hospital, Lung Cancer Prevention and Research Center of Hebei Province, Shijiazhuang, Hebei, People's Republic of China Department of Oncology, Hebei Chest Hospital, Lung Cancer Prevention and Research Center of Hebei Province, Shijiazhuang, Hebei, People's Republic of China Department of Oncology, Hebei Chest Hospital, Lung Cancer Prevention and Research Center of Hebei Province, Shijiazhuang, Hebei, People's Republic of China Department of Pathology, Hebei Chest Hospital, Lung Cancer Prevention and Research Center of Hebei Province, Shijiazhuang, Hebei, People's Republic of China College of Lab Medicine, , Zhangjiakou, Hebei, People's Republic of China Department of Oncology, Hebei Chest Hospital, Lung Cancer Prevention and Research Center of Hebei Province, Shijiazhuang, Hebei, People's Republic of China Department of Oncology, Hebei Chest Hospital, Lung Cancer Prevention and Research Center of Hebei Province, Shijiazhuang, Hebei, People's Republic of China Department of Orthopedic Surgery and BME-Campbell Clinic, University of Tennessee Health Science Center, Memphis, TN, USA Department of Internal Medicine, Hebei Chest Hospital, Lung Cancer Prevention and Research Center of Hebei Province, Shijiazhuang, Hebei, People's Republic of ChinaObjective Circulating tumor cells are complete tumor cells with multi-scale analysis values that present a high potential for lung cancer diagnosis. To enhance the accuracy of lung cancer diagnosis, we detected circulating tumor cells by the innovated conical micro filter integrated microfluidic system. Methods We recruited 45 subjects of study, including 22 lung cancer patients, 2 precancerous patients, the control group including 14 healthy participants, and 7 patients with lung benign lesions in this prospective study. We calculated the area under the receiver operating characteristic curve of circulating tumor cells, cytokeratin19 fragment, carcinoma embryonic antigen, squamous cell carcinoma, neuron-specific enolase, and their combination, respectively, while compared the circulating tumor cells levels between vein blood and arterial blood. A conical shape filter embedded in a microfluidic chip was used to improve the detection capability of circulating tumor cells. Results The study indicated that the sensitivity, specificity, positive predictive value, and negative predictive value of circulating tumor cells detection were 81.8%, 90.5%, 90.0%, and 82.6%, respectively. The circulating tumor cells level of lung cancer patient was significantly higher than that of the control group ( P < .05). The area under the curve of circulating tumor cells, cytokeratin19 fragment, carcinoma embryonic antigen, squamous cell carcinoma, and neuron-specific enolase alone was 0.838, 0.760, 0.705, 0.614, and 0.636, respectively. The combination area under the curve of the 4 tumor markers (cytokeratin19 fragment, carcinoma embryonic antigen, squamous cell carcinoma, and neuron-specific enolase) was 0.805 less than that of circulating tumor cells alone. Together, the total area under the curve of circulating tumor cell and the 4 tumor markers were 0.847, showing the highest area under the curve value among all biomarkers. In addition, this study found that there was no significant difference in positive rate of circulating tumor cell between arterial and venous blood samples. Conclusion The circulating tumor cells detection technology by conical micro filter integrated microfluidic could be used for lung cancer diagnosis with high sensitivity and specificity. Complementary combination of circulating tumor cells and conventional 4 lung cancer markers could enhance the clinical application accuracy. Venous blood should be used as a routine sample for circulating tumor cells detections.https://doi.org/10.1177/15330338231166754 |
| spellingShingle | Sumin Guo MD Jingyu Chen BS Po Hu MD Chen Li MD Xiang Wang MD Ning Chen PhD Jiale Sun Yongfeng Wang BS Jianling Wang BS Weikuan Gu PhD Shucai Wu MD The Value of Circulating Tumor Cells and Tumor Markers Detection in Lung Cancer Diagnosis Technology in Cancer Research & Treatment |
| title | The Value of Circulating Tumor Cells and Tumor Markers Detection in Lung Cancer Diagnosis |
| title_full | The Value of Circulating Tumor Cells and Tumor Markers Detection in Lung Cancer Diagnosis |
| title_fullStr | The Value of Circulating Tumor Cells and Tumor Markers Detection in Lung Cancer Diagnosis |
| title_full_unstemmed | The Value of Circulating Tumor Cells and Tumor Markers Detection in Lung Cancer Diagnosis |
| title_short | The Value of Circulating Tumor Cells and Tumor Markers Detection in Lung Cancer Diagnosis |
| title_sort | value of circulating tumor cells and tumor markers detection in lung cancer diagnosis |
| url | https://doi.org/10.1177/15330338231166754 |
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