Dynamic changes in marker components during the stir-frying of Pharbitidis Semen, and network analysis of its potential effects on nephritis
Introduction: Pharbitidis Semen (PS) has been widely used in traditional Chinese medicine to treat several diseases such as nephritis. PS is usually stir-fried to enhance its therapeutic efficacy before use in clinical practice. However, the changes in phenolic acids during stir-frying and the mecha...
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| Format: | Article |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1123476/full |
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| author | Yuman Li Yuman Li Yuman Li Yuhe Lu Yuhe Lu Yuhe Lu Yujie Zhu Yujie Zhu Yujie Zhu Jingchun Yao Haibing Hua Jinyang Shen Jinyang Shen Jinyang Shen Xun Gao Xun Gao Xun Gao Kunming Qin Kunming Qin Kunming Qin |
| author_facet | Yuman Li Yuman Li Yuman Li Yuhe Lu Yuhe Lu Yuhe Lu Yujie Zhu Yujie Zhu Yujie Zhu Jingchun Yao Haibing Hua Jinyang Shen Jinyang Shen Jinyang Shen Xun Gao Xun Gao Xun Gao Kunming Qin Kunming Qin Kunming Qin |
| author_sort | Yuman Li |
| collection | DOAJ |
| description | Introduction: Pharbitidis Semen (PS) has been widely used in traditional Chinese medicine to treat several diseases such as nephritis. PS is usually stir-fried to enhance its therapeutic efficacy before use in clinical practice. However, the changes in phenolic acids during stir-frying and the mechanisms of their therapeutic effects on nephritis are still unclear.Methods: Here, we studied the processing-induced chemical changes and elucidated the mechanism of PS in the treatment of nephritis. We determined the levels of the 7 phenolic acids in raw PS (RPS) and stir-fried PS (SPS) using high-performance liquid chromatography, analyzed the dynamic compositional changes during stir-frying, and used network analysis and molecular docking to predict and verify compound targets and pathways corresponding to nephritis.Results: The dynamic changes in the 7 phenolic acids in PS during stir-frying are suggestive of a transesterification reaction. Pathway analysis revealed that the targets of nephritis were mainly enriched in the AGE-RAGE, hypoxia-inducible factor-1, interleukin-17, and tumor necrosis factor signaling pathways among others. Molecular docking results showed that the 7 phenolic acids had good binding ability with the key nephritic targets.Discussion: The potential pharmaceutical basis, targets, and mechanisms of PS in treating nephritis were explored. Our findings provide a scientific basis for the clinical use of PS in treating nephritis. |
| first_indexed | 2024-04-10T00:44:32Z |
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| id | doaj.art-f429c6338e134ed9bd8c5e55d3f7942b |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-10T00:44:32Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-f429c6338e134ed9bd8c5e55d3f7942b2023-03-14T04:35:00ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-03-011410.3389/fphar.2023.11234761123476Dynamic changes in marker components during the stir-frying of Pharbitidis Semen, and network analysis of its potential effects on nephritisYuman Li0Yuman Li1Yuman Li2Yuhe Lu3Yuhe Lu4Yuhe Lu5Yujie Zhu6Yujie Zhu7Yujie Zhu8Jingchun Yao9Haibing Hua10Jinyang Shen11Jinyang Shen12Jinyang Shen13Xun Gao14Xun Gao15Xun Gao16Kunming Qin17Kunming Qin18Kunming Qin19Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaSchool of Pharmacy, Jiangsu Ocean University, Lianyungang, ChinaCo-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaSchool of Pharmacy, Jiangsu Ocean University, Lianyungang, ChinaCo-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaSchool of Pharmacy, Jiangsu Ocean University, Lianyungang, ChinaCo-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, ChinaLunan Pharmaceutical Group Limited by Share Ltd, Linyi, ChinaJiangyin Hospital Affiliated to Nanjing University of Chinese Medicine, Wuxi, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaSchool of Pharmacy, Jiangsu Ocean University, Lianyungang, ChinaCo-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaSchool of Pharmacy, Jiangsu Ocean University, Lianyungang, ChinaCo-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, ChinaSchool of Pharmacy, Jiangsu Ocean University, Lianyungang, ChinaCo-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, ChinaIntroduction: Pharbitidis Semen (PS) has been widely used in traditional Chinese medicine to treat several diseases such as nephritis. PS is usually stir-fried to enhance its therapeutic efficacy before use in clinical practice. However, the changes in phenolic acids during stir-frying and the mechanisms of their therapeutic effects on nephritis are still unclear.Methods: Here, we studied the processing-induced chemical changes and elucidated the mechanism of PS in the treatment of nephritis. We determined the levels of the 7 phenolic acids in raw PS (RPS) and stir-fried PS (SPS) using high-performance liquid chromatography, analyzed the dynamic compositional changes during stir-frying, and used network analysis and molecular docking to predict and verify compound targets and pathways corresponding to nephritis.Results: The dynamic changes in the 7 phenolic acids in PS during stir-frying are suggestive of a transesterification reaction. Pathway analysis revealed that the targets of nephritis were mainly enriched in the AGE-RAGE, hypoxia-inducible factor-1, interleukin-17, and tumor necrosis factor signaling pathways among others. Molecular docking results showed that the 7 phenolic acids had good binding ability with the key nephritic targets.Discussion: The potential pharmaceutical basis, targets, and mechanisms of PS in treating nephritis were explored. Our findings provide a scientific basis for the clinical use of PS in treating nephritis.https://www.frontiersin.org/articles/10.3389/fphar.2023.1123476/fullPharbitidis Semenstir-fried processingnetwork analysismolecular dockingnephritis |
| spellingShingle | Yuman Li Yuman Li Yuman Li Yuhe Lu Yuhe Lu Yuhe Lu Yujie Zhu Yujie Zhu Yujie Zhu Jingchun Yao Haibing Hua Jinyang Shen Jinyang Shen Jinyang Shen Xun Gao Xun Gao Xun Gao Kunming Qin Kunming Qin Kunming Qin Dynamic changes in marker components during the stir-frying of Pharbitidis Semen, and network analysis of its potential effects on nephritis Frontiers in Pharmacology Pharbitidis Semen stir-fried processing network analysis molecular docking nephritis |
| title | Dynamic changes in marker components during the stir-frying of Pharbitidis Semen, and network analysis of its potential effects on nephritis |
| title_full | Dynamic changes in marker components during the stir-frying of Pharbitidis Semen, and network analysis of its potential effects on nephritis |
| title_fullStr | Dynamic changes in marker components during the stir-frying of Pharbitidis Semen, and network analysis of its potential effects on nephritis |
| title_full_unstemmed | Dynamic changes in marker components during the stir-frying of Pharbitidis Semen, and network analysis of its potential effects on nephritis |
| title_short | Dynamic changes in marker components during the stir-frying of Pharbitidis Semen, and network analysis of its potential effects on nephritis |
| title_sort | dynamic changes in marker components during the stir frying of pharbitidis semen and network analysis of its potential effects on nephritis |
| topic | Pharbitidis Semen stir-fried processing network analysis molecular docking nephritis |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1123476/full |
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