Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species
In this study, the genomes of the Weissella (W.) beninensis, W. diestrammenae, W. fabalis, W. fabaria, W. ghanensis, and W. uvarum type strains were sequenced and analyzed. Moreover, the ability of these strains to metabolize 95 carbohydrates was investigated, and the genetic determinants of such ca...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.914036/full |
| _version_ | 1811242802845057024 |
|---|---|
| author | Francesca Fanelli Marco Montemurro Daniele Chieffi Gyu-Sung Cho Charles M. A. P. Franz Anna Dell'Aquila Carlo Giuseppe Rizzello Vincenzina Fusco |
| author_facet | Francesca Fanelli Marco Montemurro Daniele Chieffi Gyu-Sung Cho Charles M. A. P. Franz Anna Dell'Aquila Carlo Giuseppe Rizzello Vincenzina Fusco |
| author_sort | Francesca Fanelli |
| collection | DOAJ |
| description | In this study, the genomes of the Weissella (W.) beninensis, W. diestrammenae, W. fabalis, W. fabaria, W. ghanensis, and W. uvarum type strains were sequenced and analyzed. Moreover, the ability of these strains to metabolize 95 carbohydrates was investigated, and the genetic determinants of such capability were searched within the sequenced genomes. 16S rRNA gene and genome-based-phylogeny of all the Weissella species described to date allowed a reassessment of the Weissella genus species groups. As a result, six distinct species groups within the genus, namely, W. beninensis, W. kandleri, W. confusa, W. halotolerans, W. oryzae, and W. paramesenteroides species groups, could be described. Phenotypic analyses provided further knowledge about the ability of the W. beninensis, W. ghanensis, W. fabaria, W. fabalis, W. uvarum, and W. diestrammenae type strains to metabolize certain carbohydrates and confirmed the interspecific diversity of the analyzed strains. Moreover, in many cases, the carbohydrate metabolism pathway and phylogenomic species group clustering overlapped. The novel insights provided in our study significantly improved the knowledge about the Weissella genus and allowed us to identify features that define the role of the analyzed type strains in fermentative processes and their biotechnological potential. |
| first_indexed | 2024-04-12T13:56:27Z |
| format | Article |
| id | doaj.art-f42b460dd9ea47e8b4246b2151a9f46f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T13:56:27Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f42b460dd9ea47e8b4246b2151a9f46f2022-12-22T03:30:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.914036914036Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella SpeciesFrancesca Fanelli0Marco Montemurro1Daniele Chieffi2Gyu-Sung Cho3Charles M. A. P. Franz4Anna Dell'Aquila5Carlo Giuseppe Rizzello6Vincenzina Fusco7National Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, ItalyDepartment of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, ItalyNational Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, ItalyDepartment of Microbiology and Biotechnology, Max Rubner-Institut, Kiel, GermanyDepartment of Microbiology and Biotechnology, Max Rubner-Institut, Kiel, GermanyNational Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, ItalyDepartment of Environmental Biology, Sapienza University of Rome, Rome, ItalyNational Research Council, Institute of Sciences of Food Production (CNR-ISPA), Bari, ItalyIn this study, the genomes of the Weissella (W.) beninensis, W. diestrammenae, W. fabalis, W. fabaria, W. ghanensis, and W. uvarum type strains were sequenced and analyzed. Moreover, the ability of these strains to metabolize 95 carbohydrates was investigated, and the genetic determinants of such capability were searched within the sequenced genomes. 16S rRNA gene and genome-based-phylogeny of all the Weissella species described to date allowed a reassessment of the Weissella genus species groups. As a result, six distinct species groups within the genus, namely, W. beninensis, W. kandleri, W. confusa, W. halotolerans, W. oryzae, and W. paramesenteroides species groups, could be described. Phenotypic analyses provided further knowledge about the ability of the W. beninensis, W. ghanensis, W. fabaria, W. fabalis, W. uvarum, and W. diestrammenae type strains to metabolize certain carbohydrates and confirmed the interspecific diversity of the analyzed strains. Moreover, in many cases, the carbohydrate metabolism pathway and phylogenomic species group clustering overlapped. The novel insights provided in our study significantly improved the knowledge about the Weissella genus and allowed us to identify features that define the role of the analyzed type strains in fermentative processes and their biotechnological potential.https://www.frontiersin.org/articles/10.3389/fmicb.2022.914036/fullWeissella spp.phylogenomicsgenomicscarbohydrate metabolismtrehalose |
| spellingShingle | Francesca Fanelli Marco Montemurro Daniele Chieffi Gyu-Sung Cho Charles M. A. P. Franz Anna Dell'Aquila Carlo Giuseppe Rizzello Vincenzina Fusco Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species Frontiers in Microbiology Weissella spp. phylogenomics genomics carbohydrate metabolism trehalose |
| title | Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species |
| title_full | Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species |
| title_fullStr | Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species |
| title_full_unstemmed | Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species |
| title_short | Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species |
| title_sort | novel insights into the phylogeny and biotechnological potential of weissella species |
| topic | Weissella spp. phylogenomics genomics carbohydrate metabolism trehalose |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.914036/full |
| work_keys_str_mv | AT francescafanelli novelinsightsintothephylogenyandbiotechnologicalpotentialofweissellaspecies AT marcomontemurro novelinsightsintothephylogenyandbiotechnologicalpotentialofweissellaspecies AT danielechieffi novelinsightsintothephylogenyandbiotechnologicalpotentialofweissellaspecies AT gyusungcho novelinsightsintothephylogenyandbiotechnologicalpotentialofweissellaspecies AT charlesmapfranz novelinsightsintothephylogenyandbiotechnologicalpotentialofweissellaspecies AT annadellaquila novelinsightsintothephylogenyandbiotechnologicalpotentialofweissellaspecies AT carlogiusepperizzello novelinsightsintothephylogenyandbiotechnologicalpotentialofweissellaspecies AT vincenzinafusco novelinsightsintothephylogenyandbiotechnologicalpotentialofweissellaspecies |