A Correlation Study on In Vitro Physiological Activities of Soybean Cultivars, 19 Individual Isoflavone Derivatives, and Genetic Characteristics
The functionality of soybeans is an important factor in the selection and utilization of excellent soybean cultivars, and isoflavones are representative functional substances in soybeans, which exhibit effects on antioxidants, estrogen activity, and cancer, and prevent cardiovascular diseases. This...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/12/2027 |
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| author | Han-Na Chu Su-Ji Lee Xiaohan Wang Sang-Hoon Lee Hye-Myeong Yoon Yu-Jin Hwang Eun-Suk Jung Yongseok Kwon Chi-Do Wee Kyeong-A Jang Haeng-Ran Kim |
| author_facet | Han-Na Chu Su-Ji Lee Xiaohan Wang Sang-Hoon Lee Hye-Myeong Yoon Yu-Jin Hwang Eun-Suk Jung Yongseok Kwon Chi-Do Wee Kyeong-A Jang Haeng-Ran Kim |
| author_sort | Han-Na Chu |
| collection | DOAJ |
| description | The functionality of soybeans is an important factor in the selection and utilization of excellent soybean cultivars, and isoflavones are representative functional substances in soybeans, which exhibit effects on antioxidants, estrogen activity, and cancer, and prevent cardiovascular diseases. This study analyzed ABTS, DPPH, estrogen, ER (ER) alpha, UCP-1, and NO inhibition activities in 48 types of soybean cultivars, as well as the relationship with 19 isolated types of individual isoflavone derivatives. Statistical analysis was conducted to find individual isoflavone derivatives affecting physiological activities, revealing the high correlation of three types of derivatives: genistein 7-O-(6″-O-acetyl)glucoside (6″-O-acetylgenistin), genistein 7-O-(2″-O-apiosyl)glucoside, and glycitein. Based on these results, 15 types of soybean cultivars were selected (one control type, seven yellow types, six black types, and one green type), which have both high physiological activities and a high content of individual isoflavone derivatives. In addition, these high correlations were further verified through a genome-wide association study (GWAS) to determine the association between activities, substances, and genetic characteristics. This study comprehensively describes the relationship between the specific physiological activities of soybean resources, individual isoflavone derivative substances, and SNPs, which will be utilized for in-depth research, such as selection of excellent soybean resources with specific physiological activities. |
| first_indexed | 2024-03-10T04:39:06Z |
| format | Article |
| id | doaj.art-f42c9c7c1fd3481d8eff89836466f032 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T04:39:06Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f42c9c7c1fd3481d8eff89836466f0322023-11-23T03:34:34ZengMDPI AGAntioxidants2076-39212021-12-011012202710.3390/antiox10122027A Correlation Study on In Vitro Physiological Activities of Soybean Cultivars, 19 Individual Isoflavone Derivatives, and Genetic CharacteristicsHan-Na Chu0Su-Ji Lee1Xiaohan Wang2Sang-Hoon Lee3Hye-Myeong Yoon4Yu-Jin Hwang5Eun-Suk Jung6Yongseok Kwon7Chi-Do Wee8Kyeong-A Jang9Haeng-Ran Kim10Department of Agro-Food Resources, National Institute of Agricultural Sciences, Wanju 55365, KoreaDepartment of Agro-Food Resources, National Institute of Agricultural Sciences, Wanju 55365, KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Jeonju 54874, KoreaDepartment of Agro-Food Resources, National Institute of Agricultural Sciences, Wanju 55365, KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, Jeonju 54874, KoreaDepartment of Agro-Food Resources, National Institute of Agricultural Sciences, Wanju 55365, KoreaDepartment of Agro-Food Resources, National Institute of Agricultural Sciences, Wanju 55365, KoreaDepartment of Agro-Food Resources, National Institute of Agricultural Sciences, Wanju 55365, KoreaDepartment of Agro-Food Resources, National Institute of Agricultural Sciences, Wanju 55365, KoreaDepartment of Agro-Food Resources, National Institute of Agricultural Sciences, Wanju 55365, KoreaDepartment of Agro-Food Resources, National Institute of Agricultural Sciences, Wanju 55365, KoreaThe functionality of soybeans is an important factor in the selection and utilization of excellent soybean cultivars, and isoflavones are representative functional substances in soybeans, which exhibit effects on antioxidants, estrogen activity, and cancer, and prevent cardiovascular diseases. This study analyzed ABTS, DPPH, estrogen, ER (ER) alpha, UCP-1, and NO inhibition activities in 48 types of soybean cultivars, as well as the relationship with 19 isolated types of individual isoflavone derivatives. Statistical analysis was conducted to find individual isoflavone derivatives affecting physiological activities, revealing the high correlation of three types of derivatives: genistein 7-O-(6″-O-acetyl)glucoside (6″-O-acetylgenistin), genistein 7-O-(2″-O-apiosyl)glucoside, and glycitein. Based on these results, 15 types of soybean cultivars were selected (one control type, seven yellow types, six black types, and one green type), which have both high physiological activities and a high content of individual isoflavone derivatives. In addition, these high correlations were further verified through a genome-wide association study (GWAS) to determine the association between activities, substances, and genetic characteristics. This study comprehensively describes the relationship between the specific physiological activities of soybean resources, individual isoflavone derivative substances, and SNPs, which will be utilized for in-depth research, such as selection of excellent soybean resources with specific physiological activities.https://www.mdpi.com/2076-3921/10/12/2027antioxidantsisoflavone derivativessoybeanestrogen activitycorrelation studyPLS-R |
| spellingShingle | Han-Na Chu Su-Ji Lee Xiaohan Wang Sang-Hoon Lee Hye-Myeong Yoon Yu-Jin Hwang Eun-Suk Jung Yongseok Kwon Chi-Do Wee Kyeong-A Jang Haeng-Ran Kim A Correlation Study on In Vitro Physiological Activities of Soybean Cultivars, 19 Individual Isoflavone Derivatives, and Genetic Characteristics Antioxidants antioxidants isoflavone derivatives soybean estrogen activity correlation study PLS-R |
| title | A Correlation Study on In Vitro Physiological Activities of Soybean Cultivars, 19 Individual Isoflavone Derivatives, and Genetic Characteristics |
| title_full | A Correlation Study on In Vitro Physiological Activities of Soybean Cultivars, 19 Individual Isoflavone Derivatives, and Genetic Characteristics |
| title_fullStr | A Correlation Study on In Vitro Physiological Activities of Soybean Cultivars, 19 Individual Isoflavone Derivatives, and Genetic Characteristics |
| title_full_unstemmed | A Correlation Study on In Vitro Physiological Activities of Soybean Cultivars, 19 Individual Isoflavone Derivatives, and Genetic Characteristics |
| title_short | A Correlation Study on In Vitro Physiological Activities of Soybean Cultivars, 19 Individual Isoflavone Derivatives, and Genetic Characteristics |
| title_sort | correlation study on in vitro physiological activities of soybean cultivars 19 individual isoflavone derivatives and genetic characteristics |
| topic | antioxidants isoflavone derivatives soybean estrogen activity correlation study PLS-R |
| url | https://www.mdpi.com/2076-3921/10/12/2027 |
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