A combined approach for single-cell mRNA and intracellular protein expression analysis
Here, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor p...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-05-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-02142-w |
| _version_ | 1818433340847423488 |
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| author | Johan Reimegård Marcel Tarbier Marcus Danielsson Jens Schuster Sathishkumar Baskaran Styliani Panagiotou Niklas Dahl Marc R. Friedländer Caroline J. Gallant |
| author_facet | Johan Reimegård Marcel Tarbier Marcus Danielsson Jens Schuster Sathishkumar Baskaran Styliani Panagiotou Niklas Dahl Marc R. Friedländer Caroline J. Gallant |
| author_sort | Johan Reimegård |
| collection | DOAJ |
| description | Here, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor predictors of protein abundances and activity in individual cells, indicating that the measurements are complementary. |
| first_indexed | 2024-12-14T16:19:33Z |
| format | Article |
| id | doaj.art-f42cc4fcda564a3198a2a80c8aee12b4 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-14T16:19:33Z |
| publishDate | 2021-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-f42cc4fcda564a3198a2a80c8aee12b42022-12-21T22:54:51ZengNature PortfolioCommunications Biology2399-36422021-05-014111110.1038/s42003-021-02142-wA combined approach for single-cell mRNA and intracellular protein expression analysisJohan Reimegård0Marcel Tarbier1Marcus Danielsson2Jens Schuster3Sathishkumar Baskaran4Styliani Panagiotou5Niklas Dahl6Marc R. Friedländer7Caroline J. Gallant8National Bioinformatics Infrastructure Sweden, Uppsala UniversityScience for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm UniversityScience for Life Laboratory, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Uppsala UniversityDepartment of Immunology, Genetics and Pathology, Uppsala UniversityScience for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm UniversityDepartment of Immunology, Genetics and Pathology, Uppsala UniversityHere, the authors present SPARC, a scalable approach for simultaneously measuring mRNA expression levels and targeted intracellular protein levels in single cells. They use SPARC to measure dynamic expression changes in human cells during neuronal differentiation and show that mRNA levels are poor predictors of protein abundances and activity in individual cells, indicating that the measurements are complementary.https://doi.org/10.1038/s42003-021-02142-w |
| spellingShingle | Johan Reimegård Marcel Tarbier Marcus Danielsson Jens Schuster Sathishkumar Baskaran Styliani Panagiotou Niklas Dahl Marc R. Friedländer Caroline J. Gallant A combined approach for single-cell mRNA and intracellular protein expression analysis Communications Biology |
| title | A combined approach for single-cell mRNA and intracellular protein expression analysis |
| title_full | A combined approach for single-cell mRNA and intracellular protein expression analysis |
| title_fullStr | A combined approach for single-cell mRNA and intracellular protein expression analysis |
| title_full_unstemmed | A combined approach for single-cell mRNA and intracellular protein expression analysis |
| title_short | A combined approach for single-cell mRNA and intracellular protein expression analysis |
| title_sort | combined approach for single cell mrna and intracellular protein expression analysis |
| url | https://doi.org/10.1038/s42003-021-02142-w |
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