Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goats
Abstract Background The effect of soybean hull feeding on the disruption of colonic epithelium barrier function was investigated in goats fed a high-concentrate diet. Twenty-one Boer goats (live weight, 32.57 ± 2.26 kg; age, 1 year) were randomly divided into three groups: low-concentrate diet (LC),...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1705-8 |
| _version_ | 1818943036076326912 |
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| author | Kai Zhang Yuanlu Tu Lipeng Gao Meijuan Meng Yunfeng Bai |
| author_facet | Kai Zhang Yuanlu Tu Lipeng Gao Meijuan Meng Yunfeng Bai |
| author_sort | Kai Zhang |
| collection | DOAJ |
| description | Abstract Background The effect of soybean hull feeding on the disruption of colonic epithelium barrier function was investigated in goats fed a high-concentrate diet. Twenty-one Boer goats (live weight, 32.57 ± 2.26 kg; age, 1 year) were randomly divided into three groups: low-concentrate diet (LC), high-concentrate diet (HC), and high-concentrate diet with soybean hulls (SH). Results We found that the rumen fluid in the LC and SH group shown a higher pH value compared with the HC group. The mRNA and protein expression levels of extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) in the colonic epithelium were significantly decreased in the SH group than in the HC group. Moreover, in goats fed the HC diet, SH treatment promoted gene expression and protein abundance of claudin-1, claudin-4, occludin, and ZO-1 in the colonic epithelium. Additionally, the injury to the colonic epithelium barrier caused by the HC diet was reversed by SH treatment. Conclusions Our results indicated that supplemental SH feeding reverses the damage to colonic epithelium tight junctions by inhibiting the MAPK signalling pathway and has a protective effect on the colonic epithelium during SARA. |
| first_indexed | 2024-12-20T07:20:56Z |
| format | Article |
| id | doaj.art-f42d24b150c6462e91dcf8821913e165 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-20T07:20:56Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-f42d24b150c6462e91dcf8821913e1652022-12-21T19:48:41ZengBMCBMC Veterinary Research1746-61482018-12-011411810.1186/s12917-018-1705-8Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goatsKai Zhang0Yuanlu Tu1Lipeng Gao2Meijuan Meng3Yunfeng Bai4Circular Agriculture Research Center, Jiangsu Academy of Agricultural SciencesCircular Agriculture Research Center, Jiangsu Academy of Agricultural SciencesCircular Agriculture Research Center, Jiangsu Academy of Agricultural SciencesCircular Agriculture Research Center, Jiangsu Academy of Agricultural SciencesCircular Agriculture Research Center, Jiangsu Academy of Agricultural SciencesAbstract Background The effect of soybean hull feeding on the disruption of colonic epithelium barrier function was investigated in goats fed a high-concentrate diet. Twenty-one Boer goats (live weight, 32.57 ± 2.26 kg; age, 1 year) were randomly divided into three groups: low-concentrate diet (LC), high-concentrate diet (HC), and high-concentrate diet with soybean hulls (SH). Results We found that the rumen fluid in the LC and SH group shown a higher pH value compared with the HC group. The mRNA and protein expression levels of extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) in the colonic epithelium were significantly decreased in the SH group than in the HC group. Moreover, in goats fed the HC diet, SH treatment promoted gene expression and protein abundance of claudin-1, claudin-4, occludin, and ZO-1 in the colonic epithelium. Additionally, the injury to the colonic epithelium barrier caused by the HC diet was reversed by SH treatment. Conclusions Our results indicated that supplemental SH feeding reverses the damage to colonic epithelium tight junctions by inhibiting the MAPK signalling pathway and has a protective effect on the colonic epithelium during SARA.http://link.springer.com/article/10.1186/s12917-018-1705-8GoatSARAColonic epitheliumSoybean hullsTight junction proteins |
| spellingShingle | Kai Zhang Yuanlu Tu Lipeng Gao Meijuan Meng Yunfeng Bai Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goats BMC Veterinary Research Goat SARA Colonic epithelium Soybean hulls Tight junction proteins |
| title | Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goats |
| title_full | Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goats |
| title_fullStr | Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goats |
| title_full_unstemmed | Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goats |
| title_short | Replacement of grains with soybean hulls ameliorates SARA-induced impairment of the colonic epithelium barrier function of goats |
| title_sort | replacement of grains with soybean hulls ameliorates sara induced impairment of the colonic epithelium barrier function of goats |
| topic | Goat SARA Colonic epithelium Soybean hulls Tight junction proteins |
| url | http://link.springer.com/article/10.1186/s12917-018-1705-8 |
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