Chemical constituents and antioxidative activity of the extracts from Dyera costulata leaves

Chloroform and n-butanol extracts from the leaves of Dyera costulata exhibited EC50 values for DPPH radical scavenging activity of 79.8±0.2 and 12.0±0.1 μg/ml, respectively. Chemical investigation of the extracts was conducted by means of column chromatography. β-Amyrin and rhamnazin were isolated f...

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Main Authors: Apai Malinee, Bamrung Jankeaw, Sanan Subhadhirasakul
Format: Article
Language:English
Published: Prince of Songkla University 2003-05-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
Online Access:http://www.sjst.psu.ac.th/journal/25-3-pdf/08antioxidativeleaves.pdf
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author Apai Malinee
Bamrung Jankeaw
Sanan Subhadhirasakul
author_facet Apai Malinee
Bamrung Jankeaw
Sanan Subhadhirasakul
author_sort Apai Malinee
collection DOAJ
description Chloroform and n-butanol extracts from the leaves of Dyera costulata exhibited EC50 values for DPPH radical scavenging activity of 79.8±0.2 and 12.0±0.1 μg/ml, respectively. Chemical investigation of the extracts was conducted by means of column chromatography. β-Amyrin and rhamnazin were isolated from the chloroform extract and quercetin-3-O-α-L-rhamnopyranoside was isolated from the n-butanol extract. The chemical structures of the isolated compounds were identified by comparison of their spectroscopic data with those reported in the literature. The EC50 value of quercetin-3-O-α-L-rhamnopyranoside in DPPH radical scavenging activity was 9.37±0.02 μM. Its antioxidative activity is therefore about 8 times higher than that of the well known antioxidant, BHT, which has an EC50 value of 80.78±0.01 μM.
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spelling doaj.art-f430ef0fd8b84ed3919b7624128856912022-12-22T03:34:07ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952003-05-01253351357Chemical constituents and antioxidative activity of the extracts from Dyera costulata leavesApai MalineeBamrung JankeawSanan SubhadhirasakulChloroform and n-butanol extracts from the leaves of Dyera costulata exhibited EC50 values for DPPH radical scavenging activity of 79.8±0.2 and 12.0±0.1 μg/ml, respectively. Chemical investigation of the extracts was conducted by means of column chromatography. β-Amyrin and rhamnazin were isolated from the chloroform extract and quercetin-3-O-α-L-rhamnopyranoside was isolated from the n-butanol extract. The chemical structures of the isolated compounds were identified by comparison of their spectroscopic data with those reported in the literature. The EC50 value of quercetin-3-O-α-L-rhamnopyranoside in DPPH radical scavenging activity was 9.37±0.02 μM. Its antioxidative activity is therefore about 8 times higher than that of the well known antioxidant, BHT, which has an EC50 value of 80.78±0.01 μM.http://www.sjst.psu.ac.th/journal/25-3-pdf/08antioxidativeleaves.pdfantioxidativeflavonequercetinrhamnazinDyera costulata
spellingShingle Apai Malinee
Bamrung Jankeaw
Sanan Subhadhirasakul
Chemical constituents and antioxidative activity of the extracts from Dyera costulata leaves
Songklanakarin Journal of Science and Technology (SJST)
antioxidative
flavone
quercetin
rhamnazin
Dyera costulata
title Chemical constituents and antioxidative activity of the extracts from Dyera costulata leaves
title_full Chemical constituents and antioxidative activity of the extracts from Dyera costulata leaves
title_fullStr Chemical constituents and antioxidative activity of the extracts from Dyera costulata leaves
title_full_unstemmed Chemical constituents and antioxidative activity of the extracts from Dyera costulata leaves
title_short Chemical constituents and antioxidative activity of the extracts from Dyera costulata leaves
title_sort chemical constituents and antioxidative activity of the extracts from dyera costulata leaves
topic antioxidative
flavone
quercetin
rhamnazin
Dyera costulata
url http://www.sjst.psu.ac.th/journal/25-3-pdf/08antioxidativeleaves.pdf
work_keys_str_mv AT apaimalinee chemicalconstituentsandantioxidativeactivityoftheextractsfromdyeracostulataleaves
AT bamrungjankeaw chemicalconstituentsandantioxidativeactivityoftheextractsfromdyeracostulataleaves
AT sanansubhadhirasakul chemicalconstituentsandantioxidativeactivityoftheextractsfromdyeracostulataleaves