1H-NMR, 1H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profiling
Abstract Background The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. Methods Metabolomic profiling, employing 1H-NMR, 1H-NMR T2-edited, and 2D-NMR spectroscopy...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2017-06-01
|
| Series: | International Journal of Bipolar Disorders |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40345-017-0088-2 |
| _version_ | 1819058785328562176 |
|---|---|
| author | Sumit Sethi Mariana Pedrini Lucas B. Rizzo Maiara Zeni-Graiff Caroline Dal Mas Ana Cláudia Cassinelli Mariane N. Noto Elson Asevedo Quirino Cordeiro João G. M. Pontes Antonio J. M. Brasil Acioly Lacerda Mirian A. F. Hayashi Ronei Poppi Ljubica Tasic Elisa Brietzke |
| author_facet | Sumit Sethi Mariana Pedrini Lucas B. Rizzo Maiara Zeni-Graiff Caroline Dal Mas Ana Cláudia Cassinelli Mariane N. Noto Elson Asevedo Quirino Cordeiro João G. M. Pontes Antonio J. M. Brasil Acioly Lacerda Mirian A. F. Hayashi Ronei Poppi Ljubica Tasic Elisa Brietzke |
| author_sort | Sumit Sethi |
| collection | DOAJ |
| description | Abstract Background The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. Methods Metabolomic profiling, employing 1H-NMR, 1H-NMR T2-edited, and 2D-NMR spectroscopy and chemometrics of human blood serum samples from patients with bipolar disorder (n = 26) compared with healthy volunteers (n = 50) was performed. Results The investigated groups presented distinct metabolic profiles, in which the main differential metabolites found in the serum sample of bipolar disorder patients compared with those from controls were lipids, lipid metabolism-related molecules (choline, myo-inositol), and some amino acids (N-acetyl-l-phenyl alanine, N-acetyl-l-aspartyl-l-glutamic acid, l-glutamine). In addition, amygdalin, α-ketoglutaric acid, and lipoamide, among other compounds, were also present or were significantly altered in the serum of bipolar disorder patients. The data presented herein suggest that some of these metabolites differentially distributed between the groups studied may be directly related to the bipolar disorder pathophysiology. Conclusions The strategy employed here showed significant potential for exploring pathophysiological features and molecular pathways involved in bipolar disorder. Thus, our findings may contribute to pave the way for future studies aiming at identifying important potential biomarkers for bipolar disorder diagnosis or progression follow-up. |
| first_indexed | 2024-12-21T14:00:43Z |
| format | Article |
| id | doaj.art-f435593e4414413c9e8b6f410b645912 |
| institution | Directory Open Access Journal |
| issn | 2194-7511 |
| language | English |
| last_indexed | 2024-12-21T14:00:43Z |
| publishDate | 2017-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | International Journal of Bipolar Disorders |
| spelling | doaj.art-f435593e4414413c9e8b6f410b6459122022-12-21T19:01:23ZengSpringerOpenInternational Journal of Bipolar Disorders2194-75112017-06-01511910.1186/s40345-017-0088-21H-NMR, 1H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profilingSumit Sethi0Mariana Pedrini1Lucas B. Rizzo2Maiara Zeni-Graiff3Caroline Dal Mas4Ana Cláudia Cassinelli5Mariane N. Noto6Elson Asevedo7Quirino Cordeiro8João G. M. Pontes9Antonio J. M. Brasil10Acioly Lacerda11Mirian A. F. Hayashi12Ronei Poppi13Ljubica Tasic14Elisa Brietzke15Department of Psychiatry, Universidade Federal de São Paulo-UNIFESPDepartment of Psychiatry, Universidade Federal de São Paulo-UNIFESPDepartment of Psychiatry, Universidade Federal de São Paulo-UNIFESPDepartment of Psychiatry, Universidade Federal de São Paulo-UNIFESPDepartment of Pharmacology, Universidade Federal de São Paulo-UNIFESPDepartment of Psychiatry, Irmandade da Santa Casa de Misericórdia de São Paulo (ISCMSP)Department of Psychiatry, Universidade Federal de São Paulo-UNIFESPDepartment of Psychiatry, Universidade Federal de São Paulo-UNIFESPDepartment of Psychiatry, Irmandade da Santa Casa de Misericórdia de São Paulo (ISCMSP)Laboratório de Química Biológica, Department of Organic Chemistry, Institute of Chemistry, Universidade Estadual de Campinas-UNICAMPLaboratório de Química Biológica, Department of Organic Chemistry, Institute of Chemistry, Universidade Estadual de Campinas-UNICAMPDepartment of Psychiatry, Universidade Federal de São Paulo-UNIFESPDepartment of Pharmacology, Universidade Federal de São Paulo-UNIFESPDepartment of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas-UNICAMPLaboratório de Química Biológica, Department of Organic Chemistry, Institute of Chemistry, Universidade Estadual de Campinas-UNICAMPDepartment of Psychiatry, Universidade Federal de São Paulo-UNIFESPAbstract Background The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. Methods Metabolomic profiling, employing 1H-NMR, 1H-NMR T2-edited, and 2D-NMR spectroscopy and chemometrics of human blood serum samples from patients with bipolar disorder (n = 26) compared with healthy volunteers (n = 50) was performed. Results The investigated groups presented distinct metabolic profiles, in which the main differential metabolites found in the serum sample of bipolar disorder patients compared with those from controls were lipids, lipid metabolism-related molecules (choline, myo-inositol), and some amino acids (N-acetyl-l-phenyl alanine, N-acetyl-l-aspartyl-l-glutamic acid, l-glutamine). In addition, amygdalin, α-ketoglutaric acid, and lipoamide, among other compounds, were also present or were significantly altered in the serum of bipolar disorder patients. The data presented herein suggest that some of these metabolites differentially distributed between the groups studied may be directly related to the bipolar disorder pathophysiology. Conclusions The strategy employed here showed significant potential for exploring pathophysiological features and molecular pathways involved in bipolar disorder. Thus, our findings may contribute to pave the way for future studies aiming at identifying important potential biomarkers for bipolar disorder diagnosis or progression follow-up.http://link.springer.com/article/10.1186/s40345-017-0088-21H-NMRBiomarkersBipolar disorderMetabolic profilingChemometrics2D NMR |
| spellingShingle | Sumit Sethi Mariana Pedrini Lucas B. Rizzo Maiara Zeni-Graiff Caroline Dal Mas Ana Cláudia Cassinelli Mariane N. Noto Elson Asevedo Quirino Cordeiro João G. M. Pontes Antonio J. M. Brasil Acioly Lacerda Mirian A. F. Hayashi Ronei Poppi Ljubica Tasic Elisa Brietzke 1H-NMR, 1H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profiling International Journal of Bipolar Disorders 1H-NMR Biomarkers Bipolar disorder Metabolic profiling Chemometrics 2D NMR |
| title | 1H-NMR, 1H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
| title_full | 1H-NMR, 1H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
| title_fullStr | 1H-NMR, 1H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
| title_full_unstemmed | 1H-NMR, 1H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
| title_short | 1H-NMR, 1H-NMR T2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
| title_sort | 1h nmr 1h nmr t2 edited and 2d nmr in bipolar disorder metabolic profiling |
| topic | 1H-NMR Biomarkers Bipolar disorder Metabolic profiling Chemometrics 2D NMR |
| url | http://link.springer.com/article/10.1186/s40345-017-0088-2 |
| work_keys_str_mv | AT sumitsethi 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT marianapedrini 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT lucasbrizzo 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT maiarazenigraiff 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT carolinedalmas 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT anaclaudiacassinelli 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT marianennoto 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT elsonasevedo 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT quirinocordeiro 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT joaogmpontes 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT antoniojmbrasil 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT aciolylacerda 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT mirianafhayashi 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT roneipoppi 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT ljubicatasic 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling AT elisabrietzke 1hnmr1hnmrt2editedand2dnmrinbipolardisordermetabolicprofiling |