Comparative expression analysis in three Brassicaceae species revealed compensatory changes of the underlying gene regulatory network
Trichomes are regularly distributed on the leaves of Arabidopsis thaliana. The gene regulatory network underlying trichome patterning involves more than 15 genes. However, it is possible to explain patterning with only five components. This raises the questions about the function of the additional c...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-01-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1086004/full |
| _version_ | 1828072211695534080 |
|---|---|
| author | Jessica Pietsch Anna Deneer Christian Fleck Martin Hülskamp |
| author_facet | Jessica Pietsch Anna Deneer Christian Fleck Martin Hülskamp |
| author_sort | Jessica Pietsch |
| collection | DOAJ |
| description | Trichomes are regularly distributed on the leaves of Arabidopsis thaliana. The gene regulatory network underlying trichome patterning involves more than 15 genes. However, it is possible to explain patterning with only five components. This raises the questions about the function of the additional components and the identification of the core network. In this study, we compare the relative expression of all patterning genes in A. thaliana, A. alpina and C. hirsuta by qPCR analysis and use mathematical modelling to determine the relative importance of patterning genes. As the involved proteins exhibit evolutionary conserved differential complex formation, we reasoned that the genes belonging to the core network should exhibit similar expression ratios in different species. However, we find several striking differences of the relative expression levels. Our analysis of how the network can cope with such differences revealed relevant parameters that we use to predict the relevant molecular adaptations in the three species. |
| first_indexed | 2024-04-11T01:07:00Z |
| format | Article |
| id | doaj.art-f43991345be34f04b78bd66f7b636fb4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T01:07:00Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f43991345be34f04b78bd66f7b636fb42023-01-04T12:37:20ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10860041086004Comparative expression analysis in three Brassicaceae species revealed compensatory changes of the underlying gene regulatory networkJessica Pietsch0Anna Deneer1Christian Fleck2Martin Hülskamp3Botanical Institute, Biocenter, Cologne University, Cologne, GermanyBiometris, Department of Mathematical and Statistical Methods, Wageningen University, Wageningen, NetherlandsSpatial Systems Biology Group, Center for Data Analysis and Modeling, University of Freiburg, Freiburg, GermanyBotanical Institute, Biocenter, Cologne University, Cologne, GermanyTrichomes are regularly distributed on the leaves of Arabidopsis thaliana. The gene regulatory network underlying trichome patterning involves more than 15 genes. However, it is possible to explain patterning with only five components. This raises the questions about the function of the additional components and the identification of the core network. In this study, we compare the relative expression of all patterning genes in A. thaliana, A. alpina and C. hirsuta by qPCR analysis and use mathematical modelling to determine the relative importance of patterning genes. As the involved proteins exhibit evolutionary conserved differential complex formation, we reasoned that the genes belonging to the core network should exhibit similar expression ratios in different species. However, we find several striking differences of the relative expression levels. Our analysis of how the network can cope with such differences revealed relevant parameters that we use to predict the relevant molecular adaptations in the three species.https://www.frontiersin.org/articles/10.3389/fpls.2022.1086004/fullevolutiontrichomesgenetic analysispatterningbrassicaceaearabis alpina |
| spellingShingle | Jessica Pietsch Anna Deneer Christian Fleck Martin Hülskamp Comparative expression analysis in three Brassicaceae species revealed compensatory changes of the underlying gene regulatory network Frontiers in Plant Science evolution trichomes genetic analysis patterning brassicaceae arabis alpina |
| title | Comparative expression analysis in three Brassicaceae species revealed compensatory changes of the underlying gene regulatory network |
| title_full | Comparative expression analysis in three Brassicaceae species revealed compensatory changes of the underlying gene regulatory network |
| title_fullStr | Comparative expression analysis in three Brassicaceae species revealed compensatory changes of the underlying gene regulatory network |
| title_full_unstemmed | Comparative expression analysis in three Brassicaceae species revealed compensatory changes of the underlying gene regulatory network |
| title_short | Comparative expression analysis in three Brassicaceae species revealed compensatory changes of the underlying gene regulatory network |
| title_sort | comparative expression analysis in three brassicaceae species revealed compensatory changes of the underlying gene regulatory network |
| topic | evolution trichomes genetic analysis patterning brassicaceae arabis alpina |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1086004/full |
| work_keys_str_mv | AT jessicapietsch comparativeexpressionanalysisinthreebrassicaceaespeciesrevealedcompensatorychangesoftheunderlyinggeneregulatorynetwork AT annadeneer comparativeexpressionanalysisinthreebrassicaceaespeciesrevealedcompensatorychangesoftheunderlyinggeneregulatorynetwork AT christianfleck comparativeexpressionanalysisinthreebrassicaceaespeciesrevealedcompensatorychangesoftheunderlyinggeneregulatorynetwork AT martinhulskamp comparativeexpressionanalysisinthreebrassicaceaespeciesrevealedcompensatorychangesoftheunderlyinggeneregulatorynetwork |