Antioxidant and Hepatoprotective Properties of Vicia cracca Against Carbon Tetrachloride Induced Oxidative Stress in Mice
Background and purpose: Vicia cracca (Papilionaceae) has 45 species in Iran. Vicia spp. are known medicinal plants. Some species in this family are well known with distinctive antioxidant activity. This investigation was designed to evaluate the antioxidant activities and hepatoprorective properties...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2018-01-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-10123-en.html |
| _version_ | 1797943823220867072 |
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| author | Mohammad Shokrzadeh Farshid Rahimi Ali Ziar Mohammad Ali Ebrahimzadeh |
| author_facet | Mohammad Shokrzadeh Farshid Rahimi Ali Ziar Mohammad Ali Ebrahimzadeh |
| author_sort | Mohammad Shokrzadeh |
| collection | DOAJ |
| description | Background and purpose: Vicia cracca (Papilionaceae) has 45 species in Iran. Vicia spp. are known medicinal plants. Some species in this family are well known with distinctive antioxidant activity. This investigation was designed to evaluate the antioxidant activities and hepatoprorective properties of V. cracca in mice.
Materials and methods: The aerial parts were extracted by maceration method using methanol as solvent. Antioxidative capacity was assessed by utilizing three different methods: DPPH, nitric oxide (NO) free radicals scavenging, and reducing power. Hepatoprotective activity was evaluated in 50, 100 and 200 mg/kg in CCl4 induced oxidative toxicity. Also, the levels of serum ALT, AST and ALP and hepatic glutathione were measured.
Results: Total phenolic content was 17.2 ± 0.9 mg GAE and flavonoid content was 9.78 ± 0.25 mg QE. In DPPH radical scavenging activity, the IC50 value was 1.98 ± 0.25 mg ml-1. The extract showed good reducing power activity indicating significant difference with that of the positive controls (P< 0.05). The extract increased liver glutation content and decreased ALT, AST and ALP levels in mice serum. These effects were dose dependent. The results of histopathology showed a significant decrease in inflammation and necrosis in liver tissues.
Conclusion: The findings clearly indicated moderately good antioxidant capacities and potent hepatoprotective properties of the Vicia cracca extract. |
| first_indexed | 2024-04-10T20:29:07Z |
| format | Article |
| id | doaj.art-f43bf22be04a4a73a00c9c65a56fff49 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:29:07Z |
| publishDate | 2018-01-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-f43bf22be04a4a73a00c9c65a56fff492023-01-25T07:42:30ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792018-01-01271565065Antioxidant and Hepatoprotective Properties of Vicia cracca Against Carbon Tetrachloride Induced Oxidative Stress in MiceMohammad Shokrzadeh0Farshid Rahimi1Ali Ziar2Mohammad Ali Ebrahimzadeh3 Associate Professor, Department of Toxicology and Pharmacology, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran Pharmacy Student, Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran MSc in Toxicology, Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Professor, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Vicia cracca (Papilionaceae) has 45 species in Iran. Vicia spp. are known medicinal plants. Some species in this family are well known with distinctive antioxidant activity. This investigation was designed to evaluate the antioxidant activities and hepatoprorective properties of V. cracca in mice. Materials and methods: The aerial parts were extracted by maceration method using methanol as solvent. Antioxidative capacity was assessed by utilizing three different methods: DPPH, nitric oxide (NO) free radicals scavenging, and reducing power. Hepatoprotective activity was evaluated in 50, 100 and 200 mg/kg in CCl4 induced oxidative toxicity. Also, the levels of serum ALT, AST and ALP and hepatic glutathione were measured. Results: Total phenolic content was 17.2 ± 0.9 mg GAE and flavonoid content was 9.78 ± 0.25 mg QE. In DPPH radical scavenging activity, the IC50 value was 1.98 ± 0.25 mg ml-1. The extract showed good reducing power activity indicating significant difference with that of the positive controls (P< 0.05). The extract increased liver glutation content and decreased ALT, AST and ALP levels in mice serum. These effects were dose dependent. The results of histopathology showed a significant decrease in inflammation and necrosis in liver tissues. Conclusion: The findings clearly indicated moderately good antioxidant capacities and potent hepatoprotective properties of the Vicia cracca extract.http://jmums.mazums.ac.ir/article-1-10123-en.htmlvicia craccaantioxidantphenolflavonoidshepatoprotectionglutathionhepatic enzymesccl4 |
| spellingShingle | Mohammad Shokrzadeh Farshid Rahimi Ali Ziar Mohammad Ali Ebrahimzadeh Antioxidant and Hepatoprotective Properties of Vicia cracca Against Carbon Tetrachloride Induced Oxidative Stress in Mice Journal of Mazandaran University of Medical Sciences vicia cracca antioxidant phenol flavonoids hepatoprotection glutathion hepatic enzymes ccl4 |
| title | Antioxidant and Hepatoprotective Properties of Vicia cracca Against Carbon Tetrachloride Induced Oxidative Stress in Mice |
| title_full | Antioxidant and Hepatoprotective Properties of Vicia cracca Against Carbon Tetrachloride Induced Oxidative Stress in Mice |
| title_fullStr | Antioxidant and Hepatoprotective Properties of Vicia cracca Against Carbon Tetrachloride Induced Oxidative Stress in Mice |
| title_full_unstemmed | Antioxidant and Hepatoprotective Properties of Vicia cracca Against Carbon Tetrachloride Induced Oxidative Stress in Mice |
| title_short | Antioxidant and Hepatoprotective Properties of Vicia cracca Against Carbon Tetrachloride Induced Oxidative Stress in Mice |
| title_sort | antioxidant and hepatoprotective properties of vicia cracca against carbon tetrachloride induced oxidative stress in mice |
| topic | vicia cracca antioxidant phenol flavonoids hepatoprotection glutathion hepatic enzymes ccl4 |
| url | http://jmums.mazums.ac.ir/article-1-10123-en.html |
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