The three-way relationship of polymorphisms of porcine genes encoding terminal complement components, their differential expression, and health-related phenotypes
<p>Abstract</p> <p>Background</p> <p>The complement system is an evolutionary ancient mechanism that plays an essential role in innate immunity and contributes to the acquired immune response. Three modes of activation, known as classical, alternative and lectin pathway...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-06-01
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| Series: | BMC Proceedings |
| _version_ | 1828738485276639232 |
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| author | Wimmers Klaus Khoa Do Vo Anh Schütze Sabine Murani Eduard Ponsuksili Siriluck |
| author_facet | Wimmers Klaus Khoa Do Vo Anh Schütze Sabine Murani Eduard Ponsuksili Siriluck |
| author_sort | Wimmers Klaus |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The complement system is an evolutionary ancient mechanism that plays an essential role in innate immunity and contributes to the acquired immune response. Three modes of activation, known as classical, alternative and lectin pathway, lead to the initiation of a common terminal lytic pathway. The terminal complement components (TCCs: C6, C7, C8A, C8B, and C9) are encoded by the genes <it>C6</it>, <it>C7</it>, <it>C8A</it>, <it>C8B</it>, <it>C8G</it>, and <it>C9</it>. We aimed at experimentally testing the porcine genes encoding TCCs as candidate genes for immune competence and disease resistance by addressing the three-way relationship of genotype, health related phenotype, and mRNA expression.</p> <p>Results</p> <p>Comparative sequencing of cDNAs of animals of the breeds German Landrace, Piétrain, Hampshire, Duroc, Vietnamese Potbelly Pig, and Berlin Miniature Pig (BMP) revealed 30 SNPs (21 in protein domains, 12 with AA exchange). The promoter regions (each ~1.5 kb upstream the transcription start sites) of <it>C6</it>, <it>C7</it>, <it>C8A</it>, <it>C8G</it>, and <it>C9</it> exhibited 29 SNPs. Significant effects of the TCC encoding genes on hemolytic complement activity were shown in a cross of Duroc and BMP after vaccination against Mycoplasma hyopneumoniae, Aujeszky disease virus and PRRSV by analysis of variance using repeated measures mixed models. Family based association tests (FBAT) confirmed the associations. The promoter SNPs were associated with the relative abundance of TCC transcripts obtained by real time RT-PCR of 311 liver samples of commercial slaughter pigs. Complement gene expression showed significant relationship with the prevalence of acute and chronic lung lesions.</p> <p>Conclusions</p> <p>The analyses point to considerable variation of the porcine TCC genes and promote the genes as candidate genes for disease resistance.</p> |
| first_indexed | 2024-04-13T00:04:09Z |
| format | Article |
| id | doaj.art-f43c430dc61648339ef9bb3ba75542aa |
| institution | Directory Open Access Journal |
| issn | 1753-6561 |
| language | English |
| last_indexed | 2024-04-13T00:04:09Z |
| publishDate | 2011-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Proceedings |
| spelling | doaj.art-f43c430dc61648339ef9bb3ba75542aa2022-12-22T03:11:16ZengBMCBMC Proceedings1753-65612011-06-015Suppl 4S1910.1186/1753-6561-5-S4-S19The three-way relationship of polymorphisms of porcine genes encoding terminal complement components, their differential expression, and health-related phenotypesWimmers KlausKhoa Do Vo AnhSchütze SabineMurani EduardPonsuksili Siriluck<p>Abstract</p> <p>Background</p> <p>The complement system is an evolutionary ancient mechanism that plays an essential role in innate immunity and contributes to the acquired immune response. Three modes of activation, known as classical, alternative and lectin pathway, lead to the initiation of a common terminal lytic pathway. The terminal complement components (TCCs: C6, C7, C8A, C8B, and C9) are encoded by the genes <it>C6</it>, <it>C7</it>, <it>C8A</it>, <it>C8B</it>, <it>C8G</it>, and <it>C9</it>. We aimed at experimentally testing the porcine genes encoding TCCs as candidate genes for immune competence and disease resistance by addressing the three-way relationship of genotype, health related phenotype, and mRNA expression.</p> <p>Results</p> <p>Comparative sequencing of cDNAs of animals of the breeds German Landrace, Piétrain, Hampshire, Duroc, Vietnamese Potbelly Pig, and Berlin Miniature Pig (BMP) revealed 30 SNPs (21 in protein domains, 12 with AA exchange). The promoter regions (each ~1.5 kb upstream the transcription start sites) of <it>C6</it>, <it>C7</it>, <it>C8A</it>, <it>C8G</it>, and <it>C9</it> exhibited 29 SNPs. Significant effects of the TCC encoding genes on hemolytic complement activity were shown in a cross of Duroc and BMP after vaccination against Mycoplasma hyopneumoniae, Aujeszky disease virus and PRRSV by analysis of variance using repeated measures mixed models. Family based association tests (FBAT) confirmed the associations. The promoter SNPs were associated with the relative abundance of TCC transcripts obtained by real time RT-PCR of 311 liver samples of commercial slaughter pigs. Complement gene expression showed significant relationship with the prevalence of acute and chronic lung lesions.</p> <p>Conclusions</p> <p>The analyses point to considerable variation of the porcine TCC genes and promote the genes as candidate genes for disease resistance.</p> |
| spellingShingle | Wimmers Klaus Khoa Do Vo Anh Schütze Sabine Murani Eduard Ponsuksili Siriluck The three-way relationship of polymorphisms of porcine genes encoding terminal complement components, their differential expression, and health-related phenotypes BMC Proceedings |
| title | The three-way relationship of polymorphisms of porcine genes encoding terminal complement components, their differential expression, and health-related phenotypes |
| title_full | The three-way relationship of polymorphisms of porcine genes encoding terminal complement components, their differential expression, and health-related phenotypes |
| title_fullStr | The three-way relationship of polymorphisms of porcine genes encoding terminal complement components, their differential expression, and health-related phenotypes |
| title_full_unstemmed | The three-way relationship of polymorphisms of porcine genes encoding terminal complement components, their differential expression, and health-related phenotypes |
| title_short | The three-way relationship of polymorphisms of porcine genes encoding terminal complement components, their differential expression, and health-related phenotypes |
| title_sort | three way relationship of polymorphisms of porcine genes encoding terminal complement components their differential expression and health related phenotypes |
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