Epidemiological and molecular profile of rickettsiosis in Peruvian border locations
Objectives. To determine the circulation of Rickettsia in the years 2010 and 2011 in border locations in four regions of Peru and their clinical epidemiological and molecular characteristics. Materials and Methods. A cross-sectional study was carried out in Tumbes, Tacna, Madre de Dios, and Loreto....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Spanish |
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Instituto Nacional de Salud
2017-03-01
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| Series: | Revista Peruana de Medicina Experimental y Salud Pública |
| Subjects: | |
| Online Access: | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2769 |
| _version_ | 1819015754833461248 |
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| author | Rosa Palacios-Salvatierra Elizabeth Anaya-Ramírez Julio Juscamayta-López Omar Cáceres-Rey Leonardo Mendoza-Uribe Patricia Mosquera-Visaloth Fátima Conceição-Silva |
| author_facet | Rosa Palacios-Salvatierra Elizabeth Anaya-Ramírez Julio Juscamayta-López Omar Cáceres-Rey Leonardo Mendoza-Uribe Patricia Mosquera-Visaloth Fátima Conceição-Silva |
| author_sort | Rosa Palacios-Salvatierra |
| collection | DOAJ |
| description | Objectives. To determine the circulation of Rickettsia in the years 2010 and 2011 in border locations in four regions of Peru and their clinical epidemiological and molecular characteristics. Materials and Methods. A cross-sectional study was carried out in Tumbes, Tacna, Madre de Dios, and Loreto. Whole blood samples were obtained from participants for culture and indirect immunofluorescence (IIF) testing. The DNA taken from leukocytes and ectoparasite cultures was used, and those genes detected for Rickettsia that were successfully amplified were sequenced and analyzed. Results. A total of 33.8% of those surveyed carried Rickettsia antibodies (21.7% in Loreto, 33.0% in Madre de Dios, 48.2% in Tacna, and 33.3% in Tumbes). Seropositivity was confirmed with IIF in over 40% of isolates. Molecular tests showed the presence of Rickettsia felis in Ctenocephalides felis of dogs and cats in Tacna and a recently reported species for Latin America, Candidatus Rickettsia asemboensis, in fleas of cats and dogs in Loreto, Madre de Dios, and Tacna. Of the population studied, 81.4% reported a history of contact with ectoparasites, 22.6% were asymptomatic, and 27.8% lived in earthen-floored homes without water or drainage. Conclusions. Serological and molecular evidence confirms the circulation of Rickettsia in the border locations studied, with predisposing epidemiological factors. Tests confirm the presence of two species, Rickettsia felis and Candidatus Rickettsia asemboensis, which represent a potential threat to the health of the inhabitants. |
| first_indexed | 2024-12-21T02:36:46Z |
| format | Article |
| id | doaj.art-f43e41b64c1345519813e2c4b5b98e85 |
| institution | Directory Open Access Journal |
| issn | 1726-4634 1726-4642 |
| language | Spanish |
| last_indexed | 2024-12-21T02:36:46Z |
| publishDate | 2017-03-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Revista Peruana de Medicina Experimental y Salud Pública |
| spelling | doaj.art-f43e41b64c1345519813e2c4b5b98e852022-12-21T19:18:47ZspaInstituto Nacional de SaludRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422017-03-01341768410.17843/rpmesp.2017.341.27691867Epidemiological and molecular profile of rickettsiosis in Peruvian border locationsRosa Palacios-Salvatierra0Elizabeth Anaya-Ramírez1Julio Juscamayta-López2Omar Cáceres-Rey3Leonardo Mendoza-Uribe4Patricia Mosquera-Visaloth5Fátima Conceição-Silva6Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. Bióloga, magister en Salud PúblicaCentro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. Bióloga, magister en Salud PúblicaCentro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. biólogo genetista, magister en Biología MolecularCentro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. biólogo, magister en Biología Molecular.Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. BiólogoCentro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. técnica de laboratorioLaboratorio de Inmunoparasitología, Instituto Oswaldo Cruz – Fio Cruz – Rio de Janeiro, Brasil. médica, doctora en CienciasObjectives. To determine the circulation of Rickettsia in the years 2010 and 2011 in border locations in four regions of Peru and their clinical epidemiological and molecular characteristics. Materials and Methods. A cross-sectional study was carried out in Tumbes, Tacna, Madre de Dios, and Loreto. Whole blood samples were obtained from participants for culture and indirect immunofluorescence (IIF) testing. The DNA taken from leukocytes and ectoparasite cultures was used, and those genes detected for Rickettsia that were successfully amplified were sequenced and analyzed. Results. A total of 33.8% of those surveyed carried Rickettsia antibodies (21.7% in Loreto, 33.0% in Madre de Dios, 48.2% in Tacna, and 33.3% in Tumbes). Seropositivity was confirmed with IIF in over 40% of isolates. Molecular tests showed the presence of Rickettsia felis in Ctenocephalides felis of dogs and cats in Tacna and a recently reported species for Latin America, Candidatus Rickettsia asemboensis, in fleas of cats and dogs in Loreto, Madre de Dios, and Tacna. Of the population studied, 81.4% reported a history of contact with ectoparasites, 22.6% were asymptomatic, and 27.8% lived in earthen-floored homes without water or drainage. Conclusions. Serological and molecular evidence confirms the circulation of Rickettsia in the border locations studied, with predisposing epidemiological factors. Tests confirm the presence of two species, Rickettsia felis and Candidatus Rickettsia asemboensis, which represent a potential threat to the health of the inhabitants.https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2769infecciones por rickettsia , perú, epidemiología molecular, vectores artrópodos |
| spellingShingle | Rosa Palacios-Salvatierra Elizabeth Anaya-Ramírez Julio Juscamayta-López Omar Cáceres-Rey Leonardo Mendoza-Uribe Patricia Mosquera-Visaloth Fátima Conceição-Silva Epidemiological and molecular profile of rickettsiosis in Peruvian border locations Revista Peruana de Medicina Experimental y Salud Pública infecciones por rickettsia , perú, epidemiología molecular, vectores artrópodos |
| title | Epidemiological and molecular profile of rickettsiosis in Peruvian border locations |
| title_full | Epidemiological and molecular profile of rickettsiosis in Peruvian border locations |
| title_fullStr | Epidemiological and molecular profile of rickettsiosis in Peruvian border locations |
| title_full_unstemmed | Epidemiological and molecular profile of rickettsiosis in Peruvian border locations |
| title_short | Epidemiological and molecular profile of rickettsiosis in Peruvian border locations |
| title_sort | epidemiological and molecular profile of rickettsiosis in peruvian border locations |
| topic | infecciones por rickettsia , perú, epidemiología molecular, vectores artrópodos |
| url | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2769 |
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