Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)
Proteins play an important role in many reproductive functions such as sperm maturation, sperm transit in the female genital tract or sperm-oocyte interaction. However, in general, little information concerning reproductive features is available in the case of aquatic animals. The present study aims...
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| Format: | Article |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.673961/full |
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| author | Mari-Carmen Fuentes-Albero Mari-Carmen Fuentes-Albero Leopoldo González-Brusi Paula Cots Chiara Luongo Silvia Abril-Sánchez José Luis Ros-Santaella Eliana Pintus Sara Ruiz-Díaz Carlos Barros-García María-Jesús Sánchez-Calabuig María-Jesús Sánchez-Calabuig Daniel García-Párraga Daniel García-Párraga Manuel Avilés Mᵃ José Izquierdo Rico Francisco Alberto García-Vázquez |
| author_facet | Mari-Carmen Fuentes-Albero Mari-Carmen Fuentes-Albero Leopoldo González-Brusi Paula Cots Chiara Luongo Silvia Abril-Sánchez José Luis Ros-Santaella Eliana Pintus Sara Ruiz-Díaz Carlos Barros-García María-Jesús Sánchez-Calabuig María-Jesús Sánchez-Calabuig Daniel García-Párraga Daniel García-Párraga Manuel Avilés Mᵃ José Izquierdo Rico Francisco Alberto García-Vázquez |
| author_sort | Mari-Carmen Fuentes-Albero |
| collection | DOAJ |
| description | Proteins play an important role in many reproductive functions such as sperm maturation, sperm transit in the female genital tract or sperm-oocyte interaction. However, in general, little information concerning reproductive features is available in the case of aquatic animals. The present study aims to characterize the proteome of both spermatozoa and seminal plasma of bottlenose dolphins (Tursiops truncatus) as a model organism for cetaceans. Ejaculate samples were obtained from two trained dolphins housed in an aquarium. Spermatozoa and seminal plasma were analyzed by means of proteomic analyses using an LC-MS/MS, and a list with the gene symbols corresponding to each protein was submitted to the DAVID database. Of the 419 proteins identified in spermatozoa and 303 in seminal plasma, 111 proteins were shared by both. Furthermore, 70 proteins were identified as involved in reproductive processes, 39 in spermatozoa, and 31 in seminal plasma. The five most abundant proteins were also identified in these samples: AKAP3, ODF2, TUBB, GSTM3, ROPN1 for spermatozoa and CST11, LTF, ALB, HSP90B1, PIGR for seminal plasma. In conclusion, this study provides the first characterization of the proteome in cetacean sperm and seminal plasma, opening the way to future research into new biomarkers, the analysis of conservation capacity or possible additional applications in the field of assisted reproductive technologies. |
| first_indexed | 2024-12-17T02:26:02Z |
| format | Article |
| id | doaj.art-f443e0abca3c47e1a76266ba317d40b1 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-17T02:26:02Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-f443e0abca3c47e1a76266ba317d40b12022-12-21T22:07:06ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-07-01910.3389/fcell.2021.673961673961Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)Mari-Carmen Fuentes-Albero0Mari-Carmen Fuentes-Albero1Leopoldo González-Brusi2Paula Cots3Chiara Luongo4Silvia Abril-Sánchez5José Luis Ros-Santaella6Eliana Pintus7Sara Ruiz-Díaz8Carlos Barros-García9María-Jesús Sánchez-Calabuig10María-Jesús Sánchez-Calabuig11Daniel García-Párraga12Daniel García-Párraga13Manuel Avilés14Mᵃ José Izquierdo Rico15Francisco Alberto García-Vázquez16Department of Biology, Avanqua-Oceanogràfic S.L, Valencia, SpainDepartment of Physiology, Faculty of Veterinary Science, University of Murcia, Murcia, SpainDepartment of Cell Biology and Histology, Faculty of Medicine, University of Murcia, Murcia, SpainDepartment of Cell Biology and Histology, Faculty of Medicine, University of Murcia, Murcia, SpainDepartment of Physiology, Faculty of Veterinary Science, University of Murcia, Murcia, SpainDepartment of Physiology, Faculty of Veterinary Science, University of Murcia, Murcia, SpainDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaDepartment of Animal Reproduction, National Agricultural and Food Research and Technology Institute (INIA), Madrid, SpainDepartment of Biology, Avanqua-Oceanogràfic S.L, Valencia, SpainDepartment of Animal Reproduction, National Agricultural and Food Research and Technology Institute (INIA), Madrid, SpainDepartment of Medicine and Surgery, Faculty of Veterinary Science, Madrid, SpainDepartment of Biology, Avanqua-Oceanogràfic S.L, Valencia, SpainResearch Department, Fundación Oceanogràfic, Valencia, SpainDepartment of Cell Biology and Histology, Faculty of Medicine, University of Murcia, Murcia, SpainDepartment of Cell Biology and Histology, Faculty of Medicine, University of Murcia, Murcia, SpainDepartment of Physiology, Faculty of Veterinary Science, University of Murcia, Murcia, SpainProteins play an important role in many reproductive functions such as sperm maturation, sperm transit in the female genital tract or sperm-oocyte interaction. However, in general, little information concerning reproductive features is available in the case of aquatic animals. The present study aims to characterize the proteome of both spermatozoa and seminal plasma of bottlenose dolphins (Tursiops truncatus) as a model organism for cetaceans. Ejaculate samples were obtained from two trained dolphins housed in an aquarium. Spermatozoa and seminal plasma were analyzed by means of proteomic analyses using an LC-MS/MS, and a list with the gene symbols corresponding to each protein was submitted to the DAVID database. Of the 419 proteins identified in spermatozoa and 303 in seminal plasma, 111 proteins were shared by both. Furthermore, 70 proteins were identified as involved in reproductive processes, 39 in spermatozoa, and 31 in seminal plasma. The five most abundant proteins were also identified in these samples: AKAP3, ODF2, TUBB, GSTM3, ROPN1 for spermatozoa and CST11, LTF, ALB, HSP90B1, PIGR for seminal plasma. In conclusion, this study provides the first characterization of the proteome in cetacean sperm and seminal plasma, opening the way to future research into new biomarkers, the analysis of conservation capacity or possible additional applications in the field of assisted reproductive technologies.https://www.frontiersin.org/articles/10.3389/fcell.2021.673961/fullcetaceanproteomic analysessemensperm cellsseminal plasma |
| spellingShingle | Mari-Carmen Fuentes-Albero Mari-Carmen Fuentes-Albero Leopoldo González-Brusi Paula Cots Chiara Luongo Silvia Abril-Sánchez José Luis Ros-Santaella Eliana Pintus Sara Ruiz-Díaz Carlos Barros-García María-Jesús Sánchez-Calabuig María-Jesús Sánchez-Calabuig Daniel García-Párraga Daniel García-Párraga Manuel Avilés Mᵃ José Izquierdo Rico Francisco Alberto García-Vázquez Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus) Frontiers in Cell and Developmental Biology cetacean proteomic analyses semen sperm cells seminal plasma |
| title | Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus) |
| title_full | Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus) |
| title_fullStr | Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus) |
| title_full_unstemmed | Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus) |
| title_short | Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus) |
| title_sort | protein identification of spermatozoa and seminal plasma in bottlenose dolphin tursiops truncatus |
| topic | cetacean proteomic analyses semen sperm cells seminal plasma |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.673961/full |
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