Sweet Sorghum Genotypes Tolerant and Sensitive to Nitrogen Stress Select Distinct Root Endosphere and Rhizosphere Bacterial Communities
The belowground microbiomes have many beneficial functions that assist plant growth, including nutrient cycling, acquisition and transport, as well as alleviation of stresses caused by nutrient limitations such as nitrogen (N). Here we analyzed the root endosphere, rhizosphere and soil bacterial com...
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MDPI AG
2021-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1329 |
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| author | Lucas Dantas Lopes Yen Ning Chai Ellen L. Marsh John F. Rajewski Ismail Dweikat Daniel P. Schachtman |
| author_facet | Lucas Dantas Lopes Yen Ning Chai Ellen L. Marsh John F. Rajewski Ismail Dweikat Daniel P. Schachtman |
| author_sort | Lucas Dantas Lopes |
| collection | DOAJ |
| description | The belowground microbiomes have many beneficial functions that assist plant growth, including nutrient cycling, acquisition and transport, as well as alleviation of stresses caused by nutrient limitations such as nitrogen (N). Here we analyzed the root endosphere, rhizosphere and soil bacterial communities of seven sweet sorghum genotypes differing in sensitivity to N-stress. Sorghum genotypes were grown in fields with no (low-N) or sufficient (high-N) N. The dry shoot weight ratio (low-N/high-N) was used to determine N-stress sensitivity. Our hypothesis was that genotypes tolerant and sensitive to N-stress select distinct bacterial communities. The endosphere and rhizosphere bacterial community structure were significantly different between the N-stress sensitive and tolerant genotypes in the high-N field, but not in the low-N field. However, significant changes in the relative abundance of specific bacterial taxa were observed in both fields. <i>Streptomyces</i>, a bacterial genus known to alleviate plant abiotic stresses, was enriched in the endosphere and rhizosphere of the tolerant genotypes in the low-N field. Our study indicates that sweet sorghum genotypes tolerant to N-stress select taxa that can potentially mitigate the N-stress, suggesting that the interactions between N-stress tolerant lines and the root-associated microbiome might be vital for coping with N-stress. |
| first_indexed | 2024-03-10T10:17:06Z |
| format | Article |
| id | doaj.art-f44851f76b0c4fcfb7de5ba2ccf4c6ea |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:17:06Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f44851f76b0c4fcfb7de5ba2ccf4c6ea2023-11-22T00:46:34ZengMDPI AGMicroorganisms2076-26072021-06-0196132910.3390/microorganisms9061329Sweet Sorghum Genotypes Tolerant and Sensitive to Nitrogen Stress Select Distinct Root Endosphere and Rhizosphere Bacterial CommunitiesLucas Dantas Lopes0Yen Ning Chai1Ellen L. Marsh2John F. Rajewski3Ismail Dweikat4Daniel P. Schachtman5Department of Agronomy and Horticulture, Center for Plant Science Innovation, University of Nebraska—Lincoln, Lincoln, NE 68588-0660, USADepartment of Agronomy and Horticulture, Center for Plant Science Innovation, University of Nebraska—Lincoln, Lincoln, NE 68588-0660, USADepartment of Agronomy and Horticulture, Center for Plant Science Innovation, University of Nebraska—Lincoln, Lincoln, NE 68588-0660, USADepartment of Agronomy and Horticulture, Center for Plant Science Innovation, University of Nebraska—Lincoln, Lincoln, NE 68588-0660, USADepartment of Agronomy and Horticulture, Center for Plant Science Innovation, University of Nebraska—Lincoln, Lincoln, NE 68588-0660, USADepartment of Agronomy and Horticulture, Center for Plant Science Innovation, University of Nebraska—Lincoln, Lincoln, NE 68588-0660, USAThe belowground microbiomes have many beneficial functions that assist plant growth, including nutrient cycling, acquisition and transport, as well as alleviation of stresses caused by nutrient limitations such as nitrogen (N). Here we analyzed the root endosphere, rhizosphere and soil bacterial communities of seven sweet sorghum genotypes differing in sensitivity to N-stress. Sorghum genotypes were grown in fields with no (low-N) or sufficient (high-N) N. The dry shoot weight ratio (low-N/high-N) was used to determine N-stress sensitivity. Our hypothesis was that genotypes tolerant and sensitive to N-stress select distinct bacterial communities. The endosphere and rhizosphere bacterial community structure were significantly different between the N-stress sensitive and tolerant genotypes in the high-N field, but not in the low-N field. However, significant changes in the relative abundance of specific bacterial taxa were observed in both fields. <i>Streptomyces</i>, a bacterial genus known to alleviate plant abiotic stresses, was enriched in the endosphere and rhizosphere of the tolerant genotypes in the low-N field. Our study indicates that sweet sorghum genotypes tolerant to N-stress select taxa that can potentially mitigate the N-stress, suggesting that the interactions between N-stress tolerant lines and the root-associated microbiome might be vital for coping with N-stress.https://www.mdpi.com/2076-2607/9/6/1329microbial ecologysoil nitrogenrhizobiomeagricultural microbiologyplant-microbe interactions |
| spellingShingle | Lucas Dantas Lopes Yen Ning Chai Ellen L. Marsh John F. Rajewski Ismail Dweikat Daniel P. Schachtman Sweet Sorghum Genotypes Tolerant and Sensitive to Nitrogen Stress Select Distinct Root Endosphere and Rhizosphere Bacterial Communities Microorganisms microbial ecology soil nitrogen rhizobiome agricultural microbiology plant-microbe interactions |
| title | Sweet Sorghum Genotypes Tolerant and Sensitive to Nitrogen Stress Select Distinct Root Endosphere and Rhizosphere Bacterial Communities |
| title_full | Sweet Sorghum Genotypes Tolerant and Sensitive to Nitrogen Stress Select Distinct Root Endosphere and Rhizosphere Bacterial Communities |
| title_fullStr | Sweet Sorghum Genotypes Tolerant and Sensitive to Nitrogen Stress Select Distinct Root Endosphere and Rhizosphere Bacterial Communities |
| title_full_unstemmed | Sweet Sorghum Genotypes Tolerant and Sensitive to Nitrogen Stress Select Distinct Root Endosphere and Rhizosphere Bacterial Communities |
| title_short | Sweet Sorghum Genotypes Tolerant and Sensitive to Nitrogen Stress Select Distinct Root Endosphere and Rhizosphere Bacterial Communities |
| title_sort | sweet sorghum genotypes tolerant and sensitive to nitrogen stress select distinct root endosphere and rhizosphere bacterial communities |
| topic | microbial ecology soil nitrogen rhizobiome agricultural microbiology plant-microbe interactions |
| url | https://www.mdpi.com/2076-2607/9/6/1329 |
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