Lyophilized Escherichia coli strains stored for 40–50 years: morphological, serotypic, biochemical characteristics and drug sensitivity
AbstractBacterial cultures are commonly preserved for long periods of time via freeze-drying (lyophilization). Lyophilized bacteria typically retain viability from 5 to 35 years. We investigated the vitality and preservation of some of the characteristic morphological, serotypic and biochemical feat...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2023.2191737 |
| _version_ | 1797367510666838016 |
|---|---|
| author | Neli Ermenlieva Emilia Georgieva Gabriela Tsankova Gergana Nedelcheva Krasimira Laleva Sylvia Stamova Todorka Kostadinova |
| author_facet | Neli Ermenlieva Emilia Georgieva Gabriela Tsankova Gergana Nedelcheva Krasimira Laleva Sylvia Stamova Todorka Kostadinova |
| author_sort | Neli Ermenlieva |
| collection | DOAJ |
| description | AbstractBacterial cultures are commonly preserved for long periods of time via freeze-drying (lyophilization). Lyophilized bacteria typically retain viability from 5 to 35 years. We investigated the vitality and preservation of some of the characteristic morphological, serotypic and biochemical features of 14 Escherichia coli strains following lyophilized storage for over 40–50 years. We also investigated their susceptibility to conventional antibiotics used in the therapy of infections caused by Enterobacteriaceae representatives. In our study, 14 strains of E. coli related to 11 serological types – O1, O2 (two strains), O5, O7, O11, O20, O25, O26, O29, O111 (two strains) and O125 (two strains) – were used. The lyophilized microorganism ampules were produced in the period of 1971 to 1973 and were stored at 4 °C in a microbial collection for educational purposes at the Medical College – Varna, Bulgaria. Control strains were E. coli ATCC25922, an E. coli strain (used for educational purposes) and three clinical E. coli isolates from urine and wound secretions. The E. coli strains stored for 40–50 years had preserved the studied morphological and biochemical characteristics, as well as those related to their antigenic characteristics and antibiotic sensitivity. Their susceptibility to the tested antimicrobials was analogical to the control reference strain E. coli ATCC25922, indicating that despite the long storage time, all strains retained and demonstrated the typical morphological, stereotypic and biochemical characteristics of the species. |
| first_indexed | 2024-03-08T17:19:22Z |
| format | Article |
| id | doaj.art-f449e326e9d64aacaa4c509efd8e4be8 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-03-08T17:19:22Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-f449e326e9d64aacaa4c509efd8e4be82024-01-03T09:44:12ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302023-12-0137130931610.1080/13102818.2023.2191737Lyophilized Escherichia coli strains stored for 40–50 years: morphological, serotypic, biochemical characteristics and drug sensitivityNeli Ermenlieva0Emilia Georgieva1Gabriela Tsankova2Gergana Nedelcheva3Krasimira Laleva4Sylvia Stamova5Todorka Kostadinova6Department of Microbiology and Virology, Faculty of Medicine, Medical University Prof. Dr P. Stoyanov, Varna, BulgariaTraining Sector "Medical Laboratory Assistant", Medical College-Varna, Medical University Prof. Dr P. Stoyanov, Varna, BulgariaDepartment of Microbiology and Virology, Faculty of Medicine, Medical University Prof. Dr P. Stoyanov, Varna, BulgariaDepartment of Microbiology and Virology, Faculty of Medicine, Medical University Prof. Dr P. Stoyanov, Varna, BulgariaDepartment of Social Medicine and Health Care Organisation, Faculty of Public Health, Medical University Prof. Dr P. Stoyanov, Varna, BulgariaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University Prof. Dr P. Stoyanov, Varna, BulgariaDepartment of Economics and Health Care Management, Faculty of Public Health, Medical University Prof. Dr P. Stoyanov, Varna, BulgariaAbstractBacterial cultures are commonly preserved for long periods of time via freeze-drying (lyophilization). Lyophilized bacteria typically retain viability from 5 to 35 years. We investigated the vitality and preservation of some of the characteristic morphological, serotypic and biochemical features of 14 Escherichia coli strains following lyophilized storage for over 40–50 years. We also investigated their susceptibility to conventional antibiotics used in the therapy of infections caused by Enterobacteriaceae representatives. In our study, 14 strains of E. coli related to 11 serological types – O1, O2 (two strains), O5, O7, O11, O20, O25, O26, O29, O111 (two strains) and O125 (two strains) – were used. The lyophilized microorganism ampules were produced in the period of 1971 to 1973 and were stored at 4 °C in a microbial collection for educational purposes at the Medical College – Varna, Bulgaria. Control strains were E. coli ATCC25922, an E. coli strain (used for educational purposes) and three clinical E. coli isolates from urine and wound secretions. The E. coli strains stored for 40–50 years had preserved the studied morphological and biochemical characteristics, as well as those related to their antigenic characteristics and antibiotic sensitivity. Their susceptibility to the tested antimicrobials was analogical to the control reference strain E. coli ATCC25922, indicating that despite the long storage time, all strains retained and demonstrated the typical morphological, stereotypic and biochemical characteristics of the species.https://www.tandfonline.com/doi/10.1080/13102818.2023.2191737Culture storagelyophilizationsurvival of microorganismsE. colidrug sensitivity |
| spellingShingle | Neli Ermenlieva Emilia Georgieva Gabriela Tsankova Gergana Nedelcheva Krasimira Laleva Sylvia Stamova Todorka Kostadinova Lyophilized Escherichia coli strains stored for 40–50 years: morphological, serotypic, biochemical characteristics and drug sensitivity Biotechnology & Biotechnological Equipment Culture storage lyophilization survival of microorganisms E. coli drug sensitivity |
| title | Lyophilized Escherichia coli strains stored for 40–50 years: morphological, serotypic, biochemical characteristics and drug sensitivity |
| title_full | Lyophilized Escherichia coli strains stored for 40–50 years: morphological, serotypic, biochemical characteristics and drug sensitivity |
| title_fullStr | Lyophilized Escherichia coli strains stored for 40–50 years: morphological, serotypic, biochemical characteristics and drug sensitivity |
| title_full_unstemmed | Lyophilized Escherichia coli strains stored for 40–50 years: morphological, serotypic, biochemical characteristics and drug sensitivity |
| title_short | Lyophilized Escherichia coli strains stored for 40–50 years: morphological, serotypic, biochemical characteristics and drug sensitivity |
| title_sort | lyophilized escherichia coli strains stored for 40 50 years morphological serotypic biochemical characteristics and drug sensitivity |
| topic | Culture storage lyophilization survival of microorganisms E. coli drug sensitivity |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2023.2191737 |
| work_keys_str_mv | AT neliermenlieva lyophilizedescherichiacolistrainsstoredfor4050yearsmorphologicalserotypicbiochemicalcharacteristicsanddrugsensitivity AT emiliageorgieva lyophilizedescherichiacolistrainsstoredfor4050yearsmorphologicalserotypicbiochemicalcharacteristicsanddrugsensitivity AT gabrielatsankova lyophilizedescherichiacolistrainsstoredfor4050yearsmorphologicalserotypicbiochemicalcharacteristicsanddrugsensitivity AT gergananedelcheva lyophilizedescherichiacolistrainsstoredfor4050yearsmorphologicalserotypicbiochemicalcharacteristicsanddrugsensitivity AT krasimiralaleva lyophilizedescherichiacolistrainsstoredfor4050yearsmorphologicalserotypicbiochemicalcharacteristicsanddrugsensitivity AT sylviastamova lyophilizedescherichiacolistrainsstoredfor4050yearsmorphologicalserotypicbiochemicalcharacteristicsanddrugsensitivity AT todorkakostadinova lyophilizedescherichiacolistrainsstoredfor4050yearsmorphologicalserotypicbiochemicalcharacteristicsanddrugsensitivity |