Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell Cultivation
This article describes the modification conditions and properties of polymer films obtained using a solution of poly(ε-caprolactone) modified with arginine. We investigated the effects on the surface and biological properties of films created using various arginine concentrations and temperature con...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/5/1042 |
| _version_ | 1797568937867608064 |
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| author | Yuliya Nashchekina Alina Chabina Alexey Nashchekin Natalia Mikhailova |
| author_facet | Yuliya Nashchekina Alina Chabina Alexey Nashchekin Natalia Mikhailova |
| author_sort | Yuliya Nashchekina |
| collection | DOAJ |
| description | This article describes the modification conditions and properties of polymer films obtained using a solution of poly(ε-caprolactone) modified with arginine. We investigated the effects on the surface and biological properties of films created using various arginine concentrations and temperature conditions during the modification process. We found that both increasing the arginine concentration of the treatment solution or the temperature of the treatment reaction increased the arginine content of the film. Following a cellular cultivation period of 3 days, greater levels of cell proliferation were observed on all modified poly(ε-caprolactone) films compared to unmodified polymer films. Experiments using fluorescence microscopy showed that the modification conditions also had a significant effect on cellular spreading and the organization of the actin cytoskeleton following 2 h of cultivation. The degree of spreading and actin cytoskeleton organization observed in cells on these modified polymer films was superior to that of the control films. |
| first_indexed | 2024-03-10T20:05:02Z |
| format | Article |
| id | doaj.art-f44c249d152e40878812b215a2709240 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T20:05:02Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-f44c249d152e40878812b215a27092402023-11-19T23:22:10ZengMDPI AGPolymers2073-43602020-05-01125104210.3390/polym12051042Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell CultivationYuliya Nashchekina0Alina Chabina1Alexey Nashchekin2Natalia Mikhailova3Center of Cell Technologies, Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 194064, RussiaCenter of Cell Technologies, Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 194064, RussiaLaboratory Materials and structures of Solid State electronics, Ioffe Institute, Polytekhnicheskaya str., 26, St. Petersburg 194021, RussiaCenter of Cell Technologies, Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg 194064, RussiaThis article describes the modification conditions and properties of polymer films obtained using a solution of poly(ε-caprolactone) modified with arginine. We investigated the effects on the surface and biological properties of films created using various arginine concentrations and temperature conditions during the modification process. We found that both increasing the arginine concentration of the treatment solution or the temperature of the treatment reaction increased the arginine content of the film. Following a cellular cultivation period of 3 days, greater levels of cell proliferation were observed on all modified poly(ε-caprolactone) films compared to unmodified polymer films. Experiments using fluorescence microscopy showed that the modification conditions also had a significant effect on cellular spreading and the organization of the actin cytoskeleton following 2 h of cultivation. The degree of spreading and actin cytoskeleton organization observed in cells on these modified polymer films was superior to that of the control films.https://www.mdpi.com/2073-4360/12/5/1042polycaprolactone filmsargininebiofunctionalizationmesenchymal stem cells |
| spellingShingle | Yuliya Nashchekina Alina Chabina Alexey Nashchekin Natalia Mikhailova Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell Cultivation Polymers polycaprolactone films arginine biofunctionalization mesenchymal stem cells |
| title | Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell Cultivation |
| title_full | Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell Cultivation |
| title_fullStr | Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell Cultivation |
| title_full_unstemmed | Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell Cultivation |
| title_short | Polycaprolactone Films Modified by L-Arginine for Mesenchymal Stem Cell Cultivation |
| title_sort | polycaprolactone films modified by l arginine for mesenchymal stem cell cultivation |
| topic | polycaprolactone films arginine biofunctionalization mesenchymal stem cells |
| url | https://www.mdpi.com/2073-4360/12/5/1042 |
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