Clinical efficacy of intravaginal recombinant lysostaphin administration on endometritis in sows
Abstract Recombinant lysostaphin has been used for the treatment of cow endometritis and mastitis in China. To our knowledge, no scientific effort has been made to evaluate the efficacy of lysostaphin in sows with clinical endometritis. Lysostaphin, loaded in effervescent tablets that were completel...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-05-01
|
| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.417 |
| _version_ | 1818284312569577472 |
|---|---|
| author | Guizi Ye Jinjiang Huang Guodong Li Jien Zhang Yongxue Sun Dongping Zeng Wenbin Bao Jiang Zhong Qingshan Huang |
| author_facet | Guizi Ye Jinjiang Huang Guodong Li Jien Zhang Yongxue Sun Dongping Zeng Wenbin Bao Jiang Zhong Qingshan Huang |
| author_sort | Guizi Ye |
| collection | DOAJ |
| description | Abstract Recombinant lysostaphin has been used for the treatment of cow endometritis and mastitis in China. To our knowledge, no scientific effort has been made to evaluate the efficacy of lysostaphin in sows with clinical endometritis. Lysostaphin, loaded in effervescent tablets that were completely foamed and dissolved within 30 min in the presence of water or body fluids and released active lysostaphin, were administered vaginally on endometritis sows in this study. The clinical recovery, bacterial clearance and reproductive performance of sows with endometritis were investigated. We found that the 400U dosage (400U lysostaphin/pill/time, repeat once on the third day, a total of two times, with 10% oxytetracycline uterine injection as a control) is the most effective treatment. Staphylococcus aureus was the most prevalent finding (34%, n = 188), followed by Streptococcus (32%, n = 181), Escherichia coli (19%, n = 104) and other bacilli (15%, n = 83) before treatment by drugs. Administration of lysostaphin resulted in an extremely significant (p < .01) reduction in S. aureus (0.18 ± 0.25 from 4.57 ± 0.33) and Streptococcus (0.11 ± 0.14 from 3.88 ± 0.29), as well as a significant (p < .05) reduction in E. coli (0.55 ± 0.42 from 3.11 ± 0.14). Mixed infections (83%) were predominant before treatment, in contrast to single infections (61%) after treatment. Large‐scale trials were conducted to verify the clinical efficacy of lysostaphin on sow endometritis. The average cure rate of 400u lysostaphin on sow endometritis(82.5%) was higher than the antibiotic group(72.17%). In addition, our results revealed that intravaginal administration of lysostaphin had no adverse effect on the reproductive performance of sows. Thus, this lysostaphin has potential application value as a new method alternative to antibiotics to treat endometritis in sows. |
| first_indexed | 2024-12-13T00:50:48Z |
| format | Article |
| id | doaj.art-f44e88d3c4f94c9f8e3f07ec3a430706 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-12-13T00:50:48Z |
| publishDate | 2021-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-f44e88d3c4f94c9f8e3f07ec3a4307062022-12-22T00:04:56ZengWileyVeterinary Medicine and Science2053-10952021-05-017374675410.1002/vms3.417Clinical efficacy of intravaginal recombinant lysostaphin administration on endometritis in sowsGuizi Ye0Jinjiang Huang1Guodong Li2Jien Zhang3Yongxue Sun4Dongping Zeng5Wenbin Bao6Jiang Zhong7Qingshan Huang8State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai P.R.ChinaKunshan Bio‐Green Biotechnology Co., Ltd Kunshan P.R.ChinaShanghai High‐Tech United Bio‐Technological R&D Co., Ltd Shanghai ChinaShanghai High‐Tech United Bio‐Technological R&D Co., Ltd Shanghai ChinaLaboratory of Veterinary Pharmacology South China Agricultural University Guangzhou P.R.ChinaLaboratory of Veterinary Pharmacology South China Agricultural University Guangzhou P.R.ChinaYangzhou University Yangzhou P.R.ChinaState Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai P.R.ChinaState Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai P.R.ChinaAbstract Recombinant lysostaphin has been used for the treatment of cow endometritis and mastitis in China. To our knowledge, no scientific effort has been made to evaluate the efficacy of lysostaphin in sows with clinical endometritis. Lysostaphin, loaded in effervescent tablets that were completely foamed and dissolved within 30 min in the presence of water or body fluids and released active lysostaphin, were administered vaginally on endometritis sows in this study. The clinical recovery, bacterial clearance and reproductive performance of sows with endometritis were investigated. We found that the 400U dosage (400U lysostaphin/pill/time, repeat once on the third day, a total of two times, with 10% oxytetracycline uterine injection as a control) is the most effective treatment. Staphylococcus aureus was the most prevalent finding (34%, n = 188), followed by Streptococcus (32%, n = 181), Escherichia coli (19%, n = 104) and other bacilli (15%, n = 83) before treatment by drugs. Administration of lysostaphin resulted in an extremely significant (p < .01) reduction in S. aureus (0.18 ± 0.25 from 4.57 ± 0.33) and Streptococcus (0.11 ± 0.14 from 3.88 ± 0.29), as well as a significant (p < .05) reduction in E. coli (0.55 ± 0.42 from 3.11 ± 0.14). Mixed infections (83%) were predominant before treatment, in contrast to single infections (61%) after treatment. Large‐scale trials were conducted to verify the clinical efficacy of lysostaphin on sow endometritis. The average cure rate of 400u lysostaphin on sow endometritis(82.5%) was higher than the antibiotic group(72.17%). In addition, our results revealed that intravaginal administration of lysostaphin had no adverse effect on the reproductive performance of sows. Thus, this lysostaphin has potential application value as a new method alternative to antibiotics to treat endometritis in sows.https://doi.org/10.1002/vms3.417endometritisintravaginal administrationlysostaphinsow |
| spellingShingle | Guizi Ye Jinjiang Huang Guodong Li Jien Zhang Yongxue Sun Dongping Zeng Wenbin Bao Jiang Zhong Qingshan Huang Clinical efficacy of intravaginal recombinant lysostaphin administration on endometritis in sows Veterinary Medicine and Science endometritis intravaginal administration lysostaphin sow |
| title | Clinical efficacy of intravaginal recombinant lysostaphin administration on endometritis in sows |
| title_full | Clinical efficacy of intravaginal recombinant lysostaphin administration on endometritis in sows |
| title_fullStr | Clinical efficacy of intravaginal recombinant lysostaphin administration on endometritis in sows |
| title_full_unstemmed | Clinical efficacy of intravaginal recombinant lysostaphin administration on endometritis in sows |
| title_short | Clinical efficacy of intravaginal recombinant lysostaphin administration on endometritis in sows |
| title_sort | clinical efficacy of intravaginal recombinant lysostaphin administration on endometritis in sows |
| topic | endometritis intravaginal administration lysostaphin sow |
| url | https://doi.org/10.1002/vms3.417 |
| work_keys_str_mv | AT guiziye clinicalefficacyofintravaginalrecombinantlysostaphinadministrationonendometritisinsows AT jinjianghuang clinicalefficacyofintravaginalrecombinantlysostaphinadministrationonendometritisinsows AT guodongli clinicalefficacyofintravaginalrecombinantlysostaphinadministrationonendometritisinsows AT jienzhang clinicalefficacyofintravaginalrecombinantlysostaphinadministrationonendometritisinsows AT yongxuesun clinicalefficacyofintravaginalrecombinantlysostaphinadministrationonendometritisinsows AT dongpingzeng clinicalefficacyofintravaginalrecombinantlysostaphinadministrationonendometritisinsows AT wenbinbao clinicalefficacyofintravaginalrecombinantlysostaphinadministrationonendometritisinsows AT jiangzhong clinicalefficacyofintravaginalrecombinantlysostaphinadministrationonendometritisinsows AT qingshanhuang clinicalefficacyofintravaginalrecombinantlysostaphinadministrationonendometritisinsows |