The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymus
ABSTRACT: Our previous study revealed that under monochromatic red light (RL), the melatonin nuclear receptor reduces the proliferation activity of broiler thymic lymphocytes through the P65 signaling pathway. The main objective of this study was to investigate the signal mechanism by which RL decre...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123008519 |
| _version_ | 1797317488916037632 |
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| author | Juanjuan Xiong Zixu Wang Yulan Dong Jing Cao Yaoxing Chen |
| author_facet | Juanjuan Xiong Zixu Wang Yulan Dong Jing Cao Yaoxing Chen |
| author_sort | Juanjuan Xiong |
| collection | DOAJ |
| description | ABSTRACT: Our previous study revealed that under monochromatic red light (RL), the melatonin nuclear receptor reduces the proliferation activity of broiler thymic lymphocytes through the P65 signaling pathway. The main objective of this study was to investigate the signal mechanism by which RL decreases thymic lymphocyte proliferation. Initially, broilers were purchased and randomly assigned to be fed under white light (WL), red light (RL), green light (GL), and blue light (BL). Pinealectomy was performed 3 d later, and the broilers were euthanized after 14 d. The results showed that the expression of the antiapoptotic proteins Bcl-2/Bcl-xl decreased under RL, while the expression of the pro-apoptotic factor Bax/caspase-3 and the pro-inflammatory factors INF-γ/TNF-α/IL-6 increased. After pinealectomy, the expression of Bax/TNF-α/IL-6 increased in conjunction with the decrease in Bcl-2 expression. In vitro experiments demonstrated that exogenous melatonin decreased the expression of Bax/TNF-α/IL-6 in thymic lymphocytes of chicks reared under RL. This melatonin-induced effect was enhanced by SR1078 (RORα/RORγ agonist) but attenuated by SR3335 (RORα antagonist) and BAY (P65 antagonist). These findings revealed that the melatonin nuclear receptor RORα/RORγ promotes the expression of the pro-apoptotic factor Bax/caspase-3 and the pro-inflammatory factors INF-γ/TNF-α/IL-6, while inhibiting the expression of the antiapoptotic factor Bcl-2/Bcl-xl. Our research suggested the signaling pathway of monochromatic red light impacts the apoptosis of thymus lymphocytes in broiler. |
| first_indexed | 2024-03-08T03:36:43Z |
| format | Article |
| id | doaj.art-f44fbd4ea1b44c6aa53a3da025ed9c70 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-08T03:36:43Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-f44fbd4ea1b44c6aa53a3da025ed9c702024-02-10T04:43:42ZengElsevierPoultry Science0032-57912024-02-011032103331The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymusJuanjuan Xiong0Zixu Wang1Yulan Dong2Jing Cao3Yaoxing Chen4College of Animal Science and Food Engineering, Jinling Institute of Technology, Qixia, Nanjing 210046, China; Laboratory of Anatomy of Domestic Animals, College of Veterinary Medicine, China Agricultural University, Haidian, Beijing 100193, ChinaLaboratory of Anatomy of Domestic Animals, College of Veterinary Medicine, China Agricultural University, Haidian, Beijing 100193, ChinaLaboratory of Anatomy of Domestic Animals, College of Veterinary Medicine, China Agricultural University, Haidian, Beijing 100193, ChinaLaboratory of Anatomy of Domestic Animals, College of Veterinary Medicine, China Agricultural University, Haidian, Beijing 100193, ChinaLaboratory of Anatomy of Domestic Animals, College of Veterinary Medicine, China Agricultural University, Haidian, Beijing 100193, China; Corresponding author:ABSTRACT: Our previous study revealed that under monochromatic red light (RL), the melatonin nuclear receptor reduces the proliferation activity of broiler thymic lymphocytes through the P65 signaling pathway. The main objective of this study was to investigate the signal mechanism by which RL decreases thymic lymphocyte proliferation. Initially, broilers were purchased and randomly assigned to be fed under white light (WL), red light (RL), green light (GL), and blue light (BL). Pinealectomy was performed 3 d later, and the broilers were euthanized after 14 d. The results showed that the expression of the antiapoptotic proteins Bcl-2/Bcl-xl decreased under RL, while the expression of the pro-apoptotic factor Bax/caspase-3 and the pro-inflammatory factors INF-γ/TNF-α/IL-6 increased. After pinealectomy, the expression of Bax/TNF-α/IL-6 increased in conjunction with the decrease in Bcl-2 expression. In vitro experiments demonstrated that exogenous melatonin decreased the expression of Bax/TNF-α/IL-6 in thymic lymphocytes of chicks reared under RL. This melatonin-induced effect was enhanced by SR1078 (RORα/RORγ agonist) but attenuated by SR3335 (RORα antagonist) and BAY (P65 antagonist). These findings revealed that the melatonin nuclear receptor RORα/RORγ promotes the expression of the pro-apoptotic factor Bax/caspase-3 and the pro-inflammatory factors INF-γ/TNF-α/IL-6, while inhibiting the expression of the antiapoptotic factor Bcl-2/Bcl-xl. Our research suggested the signaling pathway of monochromatic red light impacts the apoptosis of thymus lymphocytes in broiler.http://www.sciencedirect.com/science/article/pii/S0032579123008519immune systemRORα/RORγmechanismred lightchick |
| spellingShingle | Juanjuan Xiong Zixu Wang Yulan Dong Jing Cao Yaoxing Chen The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymus Poultry Science immune system RORα/RORγ mechanism red light chick |
| title | The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymus |
| title_full | The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymus |
| title_fullStr | The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymus |
| title_full_unstemmed | The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymus |
| title_short | The signal pathway of melatonin mediates the monochromatic light-induced T-lymphocyte apoptosis in chicken thymus |
| title_sort | signal pathway of melatonin mediates the monochromatic light induced t lymphocyte apoptosis in chicken thymus |
| topic | immune system RORα/RORγ mechanism red light chick |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123008519 |
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