Diversity Induction of Dendrobium sylvanum Orchid through In Vitro Irradiation of Gamma Ray
Dendrobium sylvanum orchid is categorized as lowland habitus orchid and able to survive wihtout any shade. Orchid cultivation takes a very long time so it needs faster cultivation and producing large quantities. Therefore, it is necessary to do orchid culture by using tissue culture processes and th...
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| Format: | Article |
| Language: | English |
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Universitas Negeri Semarang
2018-12-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/16265 |
| _version_ | 1819153524550795264 |
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| author | Eka Puji Lestari Ahmad Yunus Sugiyarto Sugiyarto |
| author_facet | Eka Puji Lestari Ahmad Yunus Sugiyarto Sugiyarto |
| author_sort | Eka Puji Lestari |
| collection | DOAJ |
| description | Dendrobium sylvanum orchid is categorized as lowland habitus orchid and able to survive wihtout any shade. Orchid cultivation takes a very long time so it needs faster cultivation and producing large quantities. Therefore, it is necessary to do orchid culture by using tissue culture processes and then given gamma ray irradiation to bring up new characters in the orchid .The purpose of this research was to discover the impact of gamma ray irradiation in vitro to the diversity of D. sylvanum. The doses of gamma ray used in this research were 0 Gy, 15 Gy, 30 Gy, 45 Gy, and 60 Gy. The results obtained indicated that mutation mostly occurred on the treatment with 15 Gy (4.4 cm of plant height, 2.67 of leaves, 2.36 cm of leaf length, 0.49 cm of leaf width, and 5.33 of root strands) compared to the other doses and the control plants. The new finding in this research is the gamma ray dose that can optimally stimulate the mutation in D. sylvanum. This information is useful to generate the new variety in orchid cultivation in Indonesia. This research provides innovation in orchid cultivation and new variety that is possible to arise after the mutation. |
| first_indexed | 2024-12-22T15:06:33Z |
| format | Article |
| id | doaj.art-f452d9bcd31b4483ae2fb14b3a45ed7a |
| institution | Directory Open Access Journal |
| issn | 2085-191X 2338-7610 |
| language | English |
| last_indexed | 2024-12-22T15:06:33Z |
| publishDate | 2018-12-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj.art-f452d9bcd31b4483ae2fb14b3a45ed7a2022-12-21T18:21:58ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102018-12-0110369169710.15294/biosaintifika.v10i3.162658164Diversity Induction of Dendrobium sylvanum Orchid through In Vitro Irradiation of Gamma RayEka Puji Lestari0Ahmad Yunus1Sugiyarto Sugiyarto2Department of Bioscience, Postgraduate, Universitas Sebelas MaretDepartment of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, IndonesiaDepartment of Biology, Faculty of Science and Mathematics, Universitas Sebelas Maret, IndonesiaDendrobium sylvanum orchid is categorized as lowland habitus orchid and able to survive wihtout any shade. Orchid cultivation takes a very long time so it needs faster cultivation and producing large quantities. Therefore, it is necessary to do orchid culture by using tissue culture processes and then given gamma ray irradiation to bring up new characters in the orchid .The purpose of this research was to discover the impact of gamma ray irradiation in vitro to the diversity of D. sylvanum. The doses of gamma ray used in this research were 0 Gy, 15 Gy, 30 Gy, 45 Gy, and 60 Gy. The results obtained indicated that mutation mostly occurred on the treatment with 15 Gy (4.4 cm of plant height, 2.67 of leaves, 2.36 cm of leaf length, 0.49 cm of leaf width, and 5.33 of root strands) compared to the other doses and the control plants. The new finding in this research is the gamma ray dose that can optimally stimulate the mutation in D. sylvanum. This information is useful to generate the new variety in orchid cultivation in Indonesia. This research provides innovation in orchid cultivation and new variety that is possible to arise after the mutation.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/16265dendrobium sylvanumgamma ray irradiationin vitro tissue culture |
| spellingShingle | Eka Puji Lestari Ahmad Yunus Sugiyarto Sugiyarto Diversity Induction of Dendrobium sylvanum Orchid through In Vitro Irradiation of Gamma Ray Biosaintifika: Journal of Biology & Biology Education dendrobium sylvanum gamma ray irradiation in vitro tissue culture |
| title | Diversity Induction of Dendrobium sylvanum Orchid through In Vitro Irradiation of Gamma Ray |
| title_full | Diversity Induction of Dendrobium sylvanum Orchid through In Vitro Irradiation of Gamma Ray |
| title_fullStr | Diversity Induction of Dendrobium sylvanum Orchid through In Vitro Irradiation of Gamma Ray |
| title_full_unstemmed | Diversity Induction of Dendrobium sylvanum Orchid through In Vitro Irradiation of Gamma Ray |
| title_short | Diversity Induction of Dendrobium sylvanum Orchid through In Vitro Irradiation of Gamma Ray |
| title_sort | diversity induction of dendrobium sylvanum orchid through in vitro irradiation of gamma ray |
| topic | dendrobium sylvanum gamma ray irradiation in vitro tissue culture |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/16265 |
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