Isolation and identification of fungi from extreme environments in Nassiriyah city soils

The present study aimed to isolation and identification some mycoflora from 40 soil sample in 6 sites  ( Remnants of fat-born, parks, edges of the river, animal wastes, sewage and rubbish) during October 2015 to January 2016  in Nassiriyah city, Iraq. According to different environmental factors. Th...

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Main Authors: Mohammed H. Mashhad, Saad S. Hamim, Maysam Kareem Hassan
Format: Article
Language:English
Published: University of Thi-Qar 2019-04-01
Series:مجلة علوم ذي قار
Subjects:
Online Access:https://mail.jsci.utq.edu.iq/index.php/main/article/view/38
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author Mohammed H. Mashhad
Saad S. Hamim
Maysam Kareem Hassan
author_facet Mohammed H. Mashhad
Saad S. Hamim
Maysam Kareem Hassan
author_sort Mohammed H. Mashhad
collection DOAJ
description The present study aimed to isolation and identification some mycoflora from 40 soil sample in 6 sites  ( Remnants of fat-born, parks, edges of the river, animal wastes, sewage and rubbish) during October 2015 to January 2016  in Nassiriyah city, Iraq. According to different environmental factors. The present study showed  the isolated  genera from  different sits and that were include Aspergillus, Penicillium, Mucor, Rhizopus, Cladosporium, Sepedonium, Alternaria, Bipolaris, Chrysosporium, Candida, Rhododendron, Humicola, Geotrichum, Fusarium and Acremonium. They were isolated by dilution method, direct plate method, alcohol and heat treatment technique using the cultural media viz. PDA, SDA and PCA. Aspergillus represented the highest fungal isolate which represent 62 (37.12%) isolation followed by Penicillium with 47 (28.14%), Mucor 22 (13.16%), Rhizopus 15 (8.98%), Cladosporium 6 (3.59%), Sepedonium and Alternaria 3 (1.80%), Bipolaris,   Chrysosporium and Candida 2 (1.20%), and finally Rhododendron, Humicola and Geotrichum recorded the lowest fungal isolation with  one isolate (0.60%). The study results  showed that soil dilution method gave a best  fungal growth in comparison with direct plate method and alcohol and heat treatment technique in 25
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spelling doaj.art-f455e477918d4e6fb8d350e66fff6bb12023-11-26T09:41:41ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562019-04-0163Isolation and identification of fungi from extreme environments in Nassiriyah city soilsMohammed H. Mashhad0Saad S. Hamim1Maysam Kareem Hassan2Biology Department / College Science / Thi Qar universityBiology department/Science collage/Thi-Qar universityBiology Department / College Science / Thi Qar universityThe present study aimed to isolation and identification some mycoflora from 40 soil sample in 6 sites  ( Remnants of fat-born, parks, edges of the river, animal wastes, sewage and rubbish) during October 2015 to January 2016  in Nassiriyah city, Iraq. According to different environmental factors. The present study showed  the isolated  genera from  different sits and that were include Aspergillus, Penicillium, Mucor, Rhizopus, Cladosporium, Sepedonium, Alternaria, Bipolaris, Chrysosporium, Candida, Rhododendron, Humicola, Geotrichum, Fusarium and Acremonium. They were isolated by dilution method, direct plate method, alcohol and heat treatment technique using the cultural media viz. PDA, SDA and PCA. Aspergillus represented the highest fungal isolate which represent 62 (37.12%) isolation followed by Penicillium with 47 (28.14%), Mucor 22 (13.16%), Rhizopus 15 (8.98%), Cladosporium 6 (3.59%), Sepedonium and Alternaria 3 (1.80%), Bipolaris,   Chrysosporium and Candida 2 (1.20%), and finally Rhododendron, Humicola and Geotrichum recorded the lowest fungal isolation with  one isolate (0.60%). The study results  showed that soil dilution method gave a best  fungal growth in comparison with direct plate method and alcohol and heat treatment technique in 25 https://mail.jsci.utq.edu.iq/index.php/main/article/view/38FungiDilution methodPDASDAPCA
spellingShingle Mohammed H. Mashhad
Saad S. Hamim
Maysam Kareem Hassan
Isolation and identification of fungi from extreme environments in Nassiriyah city soils
مجلة علوم ذي قار
Fungi
Dilution method
PDA
SDA
PCA
title Isolation and identification of fungi from extreme environments in Nassiriyah city soils
title_full Isolation and identification of fungi from extreme environments in Nassiriyah city soils
title_fullStr Isolation and identification of fungi from extreme environments in Nassiriyah city soils
title_full_unstemmed Isolation and identification of fungi from extreme environments in Nassiriyah city soils
title_short Isolation and identification of fungi from extreme environments in Nassiriyah city soils
title_sort isolation and identification of fungi from extreme environments in nassiriyah city soils
topic Fungi
Dilution method
PDA
SDA
PCA
url https://mail.jsci.utq.edu.iq/index.php/main/article/view/38
work_keys_str_mv AT mohammedhmashhad isolationandidentificationoffungifromextremeenvironmentsinnassiriyahcitysoils
AT saadshamim isolationandidentificationoffungifromextremeenvironmentsinnassiriyahcitysoils
AT maysamkareemhassan isolationandidentificationoffungifromextremeenvironmentsinnassiriyahcitysoils