Comparative Genomic Analysis of Pseudomonas chlororaphis PCL1606 Reveals New Insight into Antifungal Compounds Involved in Biocontrol
Pseudomonas chlororaphis PCL1606 is a rhizobacterium that has biocontrol activity against many soilborne phytopathogenic fungi. The whole genome sequence of this strain was obtained using the Illumina Hiseq 2000 sequencing platform and was assembled using SOAP denovo software. The resulting 6.66-Mb...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2015-03-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-14-0326-FI |
| _version_ | 1819045939273269248 |
|---|---|
| author | Claudia E. Calderón Cayo Ramos Antonio de Vicente Francisco M. Cazorla |
| author_facet | Claudia E. Calderón Cayo Ramos Antonio de Vicente Francisco M. Cazorla |
| author_sort | Claudia E. Calderón |
| collection | DOAJ |
| description | Pseudomonas chlororaphis PCL1606 is a rhizobacterium that has biocontrol activity against many soilborne phytopathogenic fungi. The whole genome sequence of this strain was obtained using the Illumina Hiseq 2000 sequencing platform and was assembled using SOAP denovo software. The resulting 6.66-Mb complete sequence of the PCL1606 genome was further analyzed. A comparative genomic analysis using 10 plant-associated strains within the fluorescent Pseudomonas group, including the complete genome of P. chlororaphis PCL1606, revealed a diverse spectrum of traits involved in multitrophic interactions with plants and microbes as well as biological control. Phylogenetic analysis of these strains using eight housekeeping genes clearly placed strain PCL1606 into the P. chlororaphis group. The genome sequence of P. chlororaphis PCL1606 revealed the presence of sequences that were homologous to biosynthetic genes for the antifungal compounds 2-hexyl, 5-propyl resorcinol (HPR), hydrogen cyanide, and pyrrolnitrin; this is the first report of pyrrolnitrin encoding genes in this P. chlororaphis strain. Single-, double-, and triple-insertional mutants in the biosynthetic genes of each antifungal compound were used to test their roles in the production of these antifungal compounds and in antagonism and biocontrol of two fungal pathogens. The results confirmed the function of HPR in the antagonistic phenotype and in the biocontrol activity of P. chlororaphis PCL1606. |
| first_indexed | 2024-12-21T10:36:32Z |
| format | Article |
| id | doaj.art-f456a4fa7648401fb69d8043da6aa31d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T10:36:32Z |
| publishDate | 2015-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-f456a4fa7648401fb69d8043da6aa31d2022-12-21T19:07:04ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062015-03-0128324926010.1094/MPMI-10-14-0326-FIComparative Genomic Analysis of Pseudomonas chlororaphis PCL1606 Reveals New Insight into Antifungal Compounds Involved in BiocontrolClaudia E. CalderónCayo RamosAntonio de VicenteFrancisco M. CazorlaPseudomonas chlororaphis PCL1606 is a rhizobacterium that has biocontrol activity against many soilborne phytopathogenic fungi. The whole genome sequence of this strain was obtained using the Illumina Hiseq 2000 sequencing platform and was assembled using SOAP denovo software. The resulting 6.66-Mb complete sequence of the PCL1606 genome was further analyzed. A comparative genomic analysis using 10 plant-associated strains within the fluorescent Pseudomonas group, including the complete genome of P. chlororaphis PCL1606, revealed a diverse spectrum of traits involved in multitrophic interactions with plants and microbes as well as biological control. Phylogenetic analysis of these strains using eight housekeeping genes clearly placed strain PCL1606 into the P. chlororaphis group. The genome sequence of P. chlororaphis PCL1606 revealed the presence of sequences that were homologous to biosynthetic genes for the antifungal compounds 2-hexyl, 5-propyl resorcinol (HPR), hydrogen cyanide, and pyrrolnitrin; this is the first report of pyrrolnitrin encoding genes in this P. chlororaphis strain. Single-, double-, and triple-insertional mutants in the biosynthetic genes of each antifungal compound were used to test their roles in the production of these antifungal compounds and in antagonism and biocontrol of two fungal pathogens. The results confirmed the function of HPR in the antagonistic phenotype and in the biocontrol activity of P. chlororaphis PCL1606.https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-14-0326-FI |
| spellingShingle | Claudia E. Calderón Cayo Ramos Antonio de Vicente Francisco M. Cazorla Comparative Genomic Analysis of Pseudomonas chlororaphis PCL1606 Reveals New Insight into Antifungal Compounds Involved in Biocontrol Molecular Plant-Microbe Interactions |
| title | Comparative Genomic Analysis of Pseudomonas chlororaphis PCL1606 Reveals New Insight into Antifungal Compounds Involved in Biocontrol |
| title_full | Comparative Genomic Analysis of Pseudomonas chlororaphis PCL1606 Reveals New Insight into Antifungal Compounds Involved in Biocontrol |
| title_fullStr | Comparative Genomic Analysis of Pseudomonas chlororaphis PCL1606 Reveals New Insight into Antifungal Compounds Involved in Biocontrol |
| title_full_unstemmed | Comparative Genomic Analysis of Pseudomonas chlororaphis PCL1606 Reveals New Insight into Antifungal Compounds Involved in Biocontrol |
| title_short | Comparative Genomic Analysis of Pseudomonas chlororaphis PCL1606 Reveals New Insight into Antifungal Compounds Involved in Biocontrol |
| title_sort | comparative genomic analysis of pseudomonas chlororaphis pcl1606 reveals new insight into antifungal compounds involved in biocontrol |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-14-0326-FI |
| work_keys_str_mv | AT claudiaecalderon comparativegenomicanalysisofpseudomonaschlororaphispcl1606revealsnewinsightintoantifungalcompoundsinvolvedinbiocontrol AT cayoramos comparativegenomicanalysisofpseudomonaschlororaphispcl1606revealsnewinsightintoantifungalcompoundsinvolvedinbiocontrol AT antoniodevicente comparativegenomicanalysisofpseudomonaschlororaphispcl1606revealsnewinsightintoantifungalcompoundsinvolvedinbiocontrol AT franciscomcazorla comparativegenomicanalysisofpseudomonaschlororaphispcl1606revealsnewinsightintoantifungalcompoundsinvolvedinbiocontrol |