| Summary: | Background. The purpose of the work is to study uterine morphogenesis and
dynamics of the cytokine profile of the blood plasma of the reproductive period rats at
correcting of methotrexate-induced immunosuppression with imunofan. Materials and
methods. The study was carried out on 60 white mongrel mature female rats weighing
210-250 g. After synchronizing the estrous cycles, the animals were divided into 2
groups. Immunosuppression was modeled by administration of methotrexate to laboratory
animals at a dosage of 10 mg/m2 of body area at the beginning of diestrus, followed by
correction by intramuscular injection of the immunomodulatory agent imunofan at a dosage
of 50 μg according to the scheme. Rats were taken out of the experiment into the
proestrus phase (PE) of the 1st, 3rd, 5th, 7th and 9th estrous cycles, the absolute and relative
mass of the uterus, the linear characteristics of its body and horns, and the volume of the
organ were measured. Micromorphometric parameters were determined at the lightoptical
level. To establish the characteristics of the state of the immune system, the content
of mediators of intercellular interaction IL-1β, IL-2, IL-6 and TNFa in the blood
plasma of animals was determined. Results. It was found out that the studied influence
has a pronounced effect on the morphometric parameters of the uterus of white mongrel
female rats of young reproductive age, and is also accompanied by statistically significant
changes in the cytokine profile of animals. Conclusions. The results obtained indicate
a favorable effect of imunofan on the dynamics of morphometric parameters of the
uterus of mature animals as a corrector of acute immunosuppression.
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