Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato
Xanthomonas vesicatoria is one of the causal agents of bacterial spot, a disease that seriously affects the production of tomato (Solanum lycopersicum) and pepper (Capsicum annum) worldwide. In Argentina, bacterial spot is found in all tomato producing areas, with X. vesicatoria being one of the mai...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1185368/full |
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| author | María Isabel Bianco María Isabel Bianco María Agustina Ponso Jerson Garita-Cambronero Valeria Paola Conforte Valeria Paola Conforte Tadeo E. Galván Germán Dunger Gustavo M. Morales Adrián Alberto Vojnov Adrián Alberto Vojnov Ana María Romero Jaime Cubero Pablo Marcelo Yaryura |
| author_facet | María Isabel Bianco María Isabel Bianco María Agustina Ponso Jerson Garita-Cambronero Valeria Paola Conforte Valeria Paola Conforte Tadeo E. Galván Germán Dunger Gustavo M. Morales Adrián Alberto Vojnov Adrián Alberto Vojnov Ana María Romero Jaime Cubero Pablo Marcelo Yaryura |
| author_sort | María Isabel Bianco |
| collection | DOAJ |
| description | Xanthomonas vesicatoria is one of the causal agents of bacterial spot, a disease that seriously affects the production of tomato (Solanum lycopersicum) and pepper (Capsicum annum) worldwide. In Argentina, bacterial spot is found in all tomato producing areas, with X. vesicatoria being one of the main species detected in the fields. Previously, we isolated three X. vesicatoria strains BNM 208, BNM 214, and BNM 216 from tomato plants with bacterial spot, and found they differed in their ability to form biofilm and in their degree of aggressiveness. Here, the likely causes of those differences were explored through genotypic and phenotypic studies. The genomes of the three strains were sequenced and assembled, and then compared with each other and also with 12 other publicly available X. vesicatoria genomes. Phenotypic characteristics (mainly linked to biofilm formation and virulence) were studied in vitro. Our results show that the differences observed earlier between BNM 208, BNM 214, and BNM 216 may be related to the structural characteristics of the xanthan gum produced by each strain, their repertoire of type III effectors (T3Es), the presence of certain genes associated with c-di-GMP metabolism and type IV pili (T4P). These findings on the pathogenicity mechanisms of X. vesicatoria could be useful for developing bacterial spot control strategies aimed at interfering with the infection processes. |
| first_indexed | 2024-03-13T03:05:15Z |
| format | Article |
| id | doaj.art-f462ad70089646e587c3dd7aead63754 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T03:05:15Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f462ad70089646e587c3dd7aead637542023-06-27T07:27:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-06-011410.3389/fmicb.2023.11853681185368Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomatoMaría Isabel Bianco0María Isabel Bianco1María Agustina Ponso2Jerson Garita-Cambronero3Valeria Paola Conforte4Valeria Paola Conforte5Tadeo E. Galván6Germán Dunger7Gustavo M. Morales8Adrián Alberto Vojnov9Adrián Alberto Vojnov10Ana María Romero11Jaime Cubero12Pablo Marcelo Yaryura13Instituto de Ciencia y Tecnología Dr. César Milstein – Fundación Pablo Cassará – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaInstituto de Investigación en Medicina y Ciencias de la Salud, Facultad de Medicina, Universidad del Salvador, Buenos Aires, ArgentinaInstituto Multidisciplinario de Investigación y Transferencia Agroalimentario y Biotecnológica (IMITAB, UNVM-CONICET), Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas, Universidad Nacional de Villa María, Villa María, ArgentinaAsociación Nacional de Obtentores Vegetales (ANOVE), Madrid, SpainInstituto de Ciencia y Tecnología Dr. César Milstein – Fundación Pablo Cassará – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaInstituto de Investigación en Medicina y Ciencias de la Salud, Facultad de Medicina, Universidad del Salvador, Buenos Aires, ArgentinaInstituto de Ciencia y Tecnología Dr. César Milstein – Fundación Pablo Cassará – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaFacultad de Ciencias Agrarias, Instituto de Ciencias Agropecuarias del Litoral, CONICET, Universidad Nacional del Litoral, Esperanza, ArgentinaDepartamento de Química, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados, Universidad Nacional de Rio Cuarto – CONICET, Rio Cuarto, ArgentinaInstituto de Ciencia y Tecnología Dr. César Milstein – Fundación Pablo Cassará – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaInstituto de Investigación en Medicina y Ciencias de la Salud, Facultad de Medicina, Universidad del Salvador, Buenos Aires, ArgentinaCátedra de Fitopatología, Departamento de Producción Vegetal, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, ArgentinaLaboratorio de Bacteriología, Departamento de Protección Vegetal, Instituto Nacional de Investigación y Tecnología Agraria/Consejo Superior de Investigaciones Científicas (INIA/CSIC), Madrid, SpainInstituto Multidisciplinario de Investigación y Transferencia Agroalimentario y Biotecnológica (IMITAB, UNVM-CONICET), Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas, Universidad Nacional de Villa María, Villa María, ArgentinaXanthomonas vesicatoria is one of the causal agents of bacterial spot, a disease that seriously affects the production of tomato (Solanum lycopersicum) and pepper (Capsicum annum) worldwide. In Argentina, bacterial spot is found in all tomato producing areas, with X. vesicatoria being one of the main species detected in the fields. Previously, we isolated three X. vesicatoria strains BNM 208, BNM 214, and BNM 216 from tomato plants with bacterial spot, and found they differed in their ability to form biofilm and in their degree of aggressiveness. Here, the likely causes of those differences were explored through genotypic and phenotypic studies. The genomes of the three strains were sequenced and assembled, and then compared with each other and also with 12 other publicly available X. vesicatoria genomes. Phenotypic characteristics (mainly linked to biofilm formation and virulence) were studied in vitro. Our results show that the differences observed earlier between BNM 208, BNM 214, and BNM 216 may be related to the structural characteristics of the xanthan gum produced by each strain, their repertoire of type III effectors (T3Es), the presence of certain genes associated with c-di-GMP metabolism and type IV pili (T4P). These findings on the pathogenicity mechanisms of X. vesicatoria could be useful for developing bacterial spot control strategies aimed at interfering with the infection processes.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1185368/fullXanthomonas vesicatoriabacterial spotxanthanbacterial motilitytype IV piliflagellum |
| spellingShingle | María Isabel Bianco María Isabel Bianco María Agustina Ponso Jerson Garita-Cambronero Valeria Paola Conforte Valeria Paola Conforte Tadeo E. Galván Germán Dunger Gustavo M. Morales Adrián Alberto Vojnov Adrián Alberto Vojnov Ana María Romero Jaime Cubero Pablo Marcelo Yaryura Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato Frontiers in Microbiology Xanthomonas vesicatoria bacterial spot xanthan bacterial motility type IV pili flagellum |
| title | Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato |
| title_full | Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato |
| title_fullStr | Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato |
| title_full_unstemmed | Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato |
| title_short | Genomic and phenotypic insight into Xanthomonas vesicatoria strains with different aggressiveness on tomato |
| title_sort | genomic and phenotypic insight into xanthomonas vesicatoria strains with different aggressiveness on tomato |
| topic | Xanthomonas vesicatoria bacterial spot xanthan bacterial motility type IV pili flagellum |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1185368/full |
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