Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration
Multiple blood-borne pathogens infecting mammals establish close interactions with the host vascular endothelium as part of their life cycles. In this work, we investigate differences in the interactions of three Trypanosoma species: T. brucei, T. congolense and T. vivax with the blood vasculature....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | The Cell Surface |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468233023000208 |
| _version_ | 1797389447441940480 |
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| author | Sara Silva Pereira Daniela Brás Teresa Porqueddu Ana M. Nascimento Mariana De Niz |
| author_facet | Sara Silva Pereira Daniela Brás Teresa Porqueddu Ana M. Nascimento Mariana De Niz |
| author_sort | Sara Silva Pereira |
| collection | DOAJ |
| description | Multiple blood-borne pathogens infecting mammals establish close interactions with the host vascular endothelium as part of their life cycles. In this work, we investigate differences in the interactions of three Trypanosoma species: T. brucei, T. congolense and T. vivax with the blood vasculature. Infection with these species results in vastly different pathologies, including different effects on vascular homeostasis, such as changes in vascular permeability and microhemorrhages. While all three species are extracellular parasites, T. congolense is strictly intravascular, while T. brucei is capable of surviving both extra- and intravascularly. Our knowledge regarding T. vivax tropism and its capacity of migration across the vascular endothelium is unknown. In this work, we show for the first time that T. vivax parasites sequester to the vascular endothelium of most organs, and that, like T. congolense, T. vivax Y486 is largely incapable of extravasation. Infection with this parasite species results in a unique effect on vascular endothelium receptors including general downregulation of ICAM1 and ESAM, and upregulation of VCAM1, CD36 and E-selectin. Our findings on the differences between the two sequestering species (T. congolense and T. vivax) and the non-sequestering, but extravasating, T. brucei raise important questions on the relevance of sequestration to the parasite’s survival in the mammalian host, and the evolutionary relevance of both sequestration and extravasation. |
| first_indexed | 2024-03-08T22:57:05Z |
| format | Article |
| id | doaj.art-f4636e2dde82419d95b9f4eb667faa82 |
| institution | Directory Open Access Journal |
| issn | 2468-2330 |
| language | English |
| last_indexed | 2024-03-08T22:57:05Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Cell Surface |
| spelling | doaj.art-f4636e2dde82419d95b9f4eb667faa822023-12-16T06:08:44ZengElsevierThe Cell Surface2468-23302023-12-0110100113Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestrationSara Silva Pereira0Daniela Brás1Teresa Porqueddu2Ana M. Nascimento3Mariana De Niz4Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; Católica Biomedical Research Centre, Católica Medical School, Universidade Católica Portuguesa, Lisbon, Portugal; Corresponding authors at: Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; Instituto Gulbenkian de Ciência, Oeiras, PortugalInstituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; Instituto Superior Técnico, Universidade de Lisboa, Lisboa, PortugalInstituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, PortugalInstituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal; Center for Advanced Microscopy and Nikon Imaging Center, Northwestern University, Chicago, USA; Corresponding authors at: Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.Multiple blood-borne pathogens infecting mammals establish close interactions with the host vascular endothelium as part of their life cycles. In this work, we investigate differences in the interactions of three Trypanosoma species: T. brucei, T. congolense and T. vivax with the blood vasculature. Infection with these species results in vastly different pathologies, including different effects on vascular homeostasis, such as changes in vascular permeability and microhemorrhages. While all three species are extracellular parasites, T. congolense is strictly intravascular, while T. brucei is capable of surviving both extra- and intravascularly. Our knowledge regarding T. vivax tropism and its capacity of migration across the vascular endothelium is unknown. In this work, we show for the first time that T. vivax parasites sequester to the vascular endothelium of most organs, and that, like T. congolense, T. vivax Y486 is largely incapable of extravasation. Infection with this parasite species results in a unique effect on vascular endothelium receptors including general downregulation of ICAM1 and ESAM, and upregulation of VCAM1, CD36 and E-selectin. Our findings on the differences between the two sequestering species (T. congolense and T. vivax) and the non-sequestering, but extravasating, T. brucei raise important questions on the relevance of sequestration to the parasite’s survival in the mammalian host, and the evolutionary relevance of both sequestration and extravasation.http://www.sciencedirect.com/science/article/pii/S2468233023000208TrypanosomaSequestrationExtravasationIntravital microscopyParasitologyHost-pathogen interactions |
| spellingShingle | Sara Silva Pereira Daniela Brás Teresa Porqueddu Ana M. Nascimento Mariana De Niz Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration The Cell Surface Trypanosoma Sequestration Extravasation Intravital microscopy Parasitology Host-pathogen interactions |
| title | Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration |
| title_full | Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration |
| title_fullStr | Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration |
| title_full_unstemmed | Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration |
| title_short | Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration |
| title_sort | investigation of trypanosoma induced vascular damage sheds insights into trypanosoma vivax sequestration |
| topic | Trypanosoma Sequestration Extravasation Intravital microscopy Parasitology Host-pathogen interactions |
| url | http://www.sciencedirect.com/science/article/pii/S2468233023000208 |
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