Detecting Overexpression Level of HER2 Gene in NSCLC by Real-time Quantitative PCR and the 2[-Delta Delta C(T)] Method
Background and objective The evaluation of the expression level of the HER2 gene for the diagnosis and treatment of tumors is usually conducted using immunohistochemical techniques. The aim of the current study is to explore the feasibility of real-time quantitative PCR and the 2[-Delta Delta C(T)]...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2011-12-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2011.12.07 |
| _version_ | 1828178194384027648 |
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| author | Shuidong FENG Hongzhuan TAN Hongyan LING Xiuqin YUAN |
| author_facet | Shuidong FENG Hongzhuan TAN Hongyan LING Xiuqin YUAN |
| author_sort | Shuidong FENG |
| collection | DOAJ |
| description | Background and objective The evaluation of the expression level of the HER2 gene for the diagnosis and treatment of tumors is usually conducted using immunohistochemical techniques. The aim of the current study is to explore the feasibility of real-time quantitative PCR and the 2[-Delta Delta C(T)] method in detecting the level of HER2 gene overexpression in non-small cell lung cancer (NSCLC). Methods Real-time quantitative PCR and the 2[-Delta Delta C(T)] method were used to detect the level of HER2 gene overexpression in 212 lung cancer and matched non-tumor tissue specimens. Results The expression level of HER2 gene in lung cancer tissue was higher than that in the matched non-tumor tissue, with an overexpression rate of 34%. Conclusion Real-time quantitative PCR and the 2[-Delta Delta C(T)] method can be used to detect the level of HER2 gene overexpression in NSCLC. |
| first_indexed | 2024-04-12T05:11:06Z |
| format | Article |
| id | doaj.art-f464c20f00ea458187a13150b261b551 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-04-12T05:11:06Z |
| publishDate | 2011-12-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-f464c20f00ea458187a13150b261b5512022-12-22T03:46:45ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872011-12-01141293894210.3779/j.issn.1009-3419.2011.12.07Detecting Overexpression Level of HER2 Gene in NSCLC by Real-time Quantitative PCR and the 2[-Delta Delta C(T)] MethodShuidong FENGHongzhuan TANHongyan LINGXiuqin YUANBackground and objective The evaluation of the expression level of the HER2 gene for the diagnosis and treatment of tumors is usually conducted using immunohistochemical techniques. The aim of the current study is to explore the feasibility of real-time quantitative PCR and the 2[-Delta Delta C(T)] method in detecting the level of HER2 gene overexpression in non-small cell lung cancer (NSCLC). Methods Real-time quantitative PCR and the 2[-Delta Delta C(T)] method were used to detect the level of HER2 gene overexpression in 212 lung cancer and matched non-tumor tissue specimens. Results The expression level of HER2 gene in lung cancer tissue was higher than that in the matched non-tumor tissue, with an overexpression rate of 34%. Conclusion Real-time quantitative PCR and the 2[-Delta Delta C(T)] method can be used to detect the level of HER2 gene overexpression in NSCLC.http://dx.doi.org/10.3779/j.issn.1009-3419.2011.12.07Real-time quantitative PCRHER2 geneLung neoplasmsExpression |
| spellingShingle | Shuidong FENG Hongzhuan TAN Hongyan LING Xiuqin YUAN Detecting Overexpression Level of HER2 Gene in NSCLC by Real-time Quantitative PCR and the 2[-Delta Delta C(T)] Method Chinese Journal of Lung Cancer Real-time quantitative PCR HER2 gene Lung neoplasms Expression |
| title | Detecting Overexpression Level of HER2 Gene in NSCLC by Real-time Quantitative PCR and the 2[-Delta Delta C(T)] Method |
| title_full | Detecting Overexpression Level of HER2 Gene in NSCLC by Real-time Quantitative PCR and the 2[-Delta Delta C(T)] Method |
| title_fullStr | Detecting Overexpression Level of HER2 Gene in NSCLC by Real-time Quantitative PCR and the 2[-Delta Delta C(T)] Method |
| title_full_unstemmed | Detecting Overexpression Level of HER2 Gene in NSCLC by Real-time Quantitative PCR and the 2[-Delta Delta C(T)] Method |
| title_short | Detecting Overexpression Level of HER2 Gene in NSCLC by Real-time Quantitative PCR and the 2[-Delta Delta C(T)] Method |
| title_sort | detecting overexpression level of her2 gene in nsclc by real time quantitative pcr and the 2 delta delta c t method |
| topic | Real-time quantitative PCR HER2 gene Lung neoplasms Expression |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2011.12.07 |
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