Short-Term Effects of Botulinum Toxin-A Injection on the Medial Gastrocnemius Histological Features in Ambulant Children with Cerebral Palsy: A Longitudinal Pilot Study
Botulinum toxin-A (BoNT-A) injection is known to exert beneficial effects on muscle tone, joint mobility and gait in children with cerebral palsy (CP). However, recent animal and human studies have raised the concern that BoNT-A might be harmful to muscle integrity. In CP-children, the impact of BoN...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6651/16/2/69 |
| _version_ | 1797296907832262656 |
|---|---|
| author | Jorieke Deschrevel Anke Andries Karen Maes Nathalie De Beukelaer Marlies Corvelyn Lauraine Staut Hannah De Houwer Domiziana Costamagna Kaat Desloovere Anja Van Campenhout Ghislaine Gayan-Ramirez |
| author_facet | Jorieke Deschrevel Anke Andries Karen Maes Nathalie De Beukelaer Marlies Corvelyn Lauraine Staut Hannah De Houwer Domiziana Costamagna Kaat Desloovere Anja Van Campenhout Ghislaine Gayan-Ramirez |
| author_sort | Jorieke Deschrevel |
| collection | DOAJ |
| description | Botulinum toxin-A (BoNT-A) injection is known to exert beneficial effects on muscle tone, joint mobility and gait in children with cerebral palsy (CP). However, recent animal and human studies have raised the concern that BoNT-A might be harmful to muscle integrity. In CP-children, the impact of BoNT-A on muscle structure has been poorly studied, and inconsistent results have been reported. This study was aimed at determining the time course effect of a single BoNT-A administration on medial gastrocnemius (MG) morphology in CP-children. MG microbiopsies from 12 ambulant and BoNT-A-naïve CP-children (age, 3.4 (2.3) years, ranging from 2.5 to 7.8 years; seven boys and five girls; GMFCS I = 5, II = 4 and III = 3) were collected before and 3 and 6 months after BoNT-A treatment to analyze the fiber cross-sectional area (fCSA) and proportion; capillarization; and satellite cell (SC) content. Compared with the baseline, the fCSA decreased at 3 months (−14%, NS) and increased at 6 months (+13%, NS). Fiber size variability was significantly higher at 3 months (type I: +56%, <i>p</i> = 0.032; type IIa: +37%, <i>p</i> = 0.032) and 6 months (type I: +69%, <i>p</i> = 0.04; type IIa: +121%, <i>p</i> = 0.032) compared with the baseline. The higher type I proportion seen at 3 months was still present and more pronounced at 6 months (type I: +17%, <i>p</i> = 0.04; type IIx: −65%, <i>p</i> = 0.032). The capillary fiber density was reduced at 3 months (type I: −43%, NS; type II: −44%, <i>p</i> = 0.0320) but normalized at 6 months. There was a non-significant increase in SC/100 fibers at 3 months (+75%, NS) and 6 months (+40%, NS) compared with the baseline. These preliminary data suggest that BoNT-A induced alterations in the MG of children with CP, which were still present 6 months after BoNT-A injection but with signs of muscle recovery. |
| first_indexed | 2024-03-07T22:12:34Z |
| format | Article |
| id | doaj.art-f464e9445c4549c58e88044f157111c9 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-07T22:12:34Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-f464e9445c4549c58e88044f157111c92024-02-23T15:36:30ZengMDPI AGToxins2072-66512024-01-011626910.3390/toxins16020069Short-Term Effects of Botulinum Toxin-A Injection on the Medial Gastrocnemius Histological Features in Ambulant Children with Cerebral Palsy: A Longitudinal Pilot StudyJorieke Deschrevel0Anke Andries1Karen Maes2Nathalie De Beukelaer3Marlies Corvelyn4Lauraine Staut5Hannah De Houwer6Domiziana Costamagna7Kaat Desloovere8Anja Van Campenhout9Ghislaine Gayan-Ramirez10Laboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Diseases and Metabolism, O&N 1bis Box 706, Herestraat 49, 3000 Leuven, BelgiumLaboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Diseases and Metabolism, O&N 1bis Box 706, Herestraat 49, 3000 Leuven, BelgiumLaboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Diseases and Metabolism, O&N 1bis Box 706, Herestraat 49, 3000 Leuven, BelgiumNeurorehabilitation Group, Department of Rehabilitation Sciences, Tervuursevest 101 Box 1501, 3000 Leuven, BelgiumStem Cell and Developmental Biology, Department of Development and Regeneration, O&N4 Box 804, 3000 Leuven, BelgiumNeurorehabilitation Group, Department of Rehabilitation Sciences, Tervuursevest 101 Box 1501, 3000 Leuven, BelgiumPediatric Orthopedics, Department of Development and Regeneration, Herestraat 49 Box 7003, 3000 Leuven, BelgiumNeurorehabilitation Group, Department of Rehabilitation Sciences, Tervuursevest 101 Box 1501, 3000 Leuven, BelgiumNeurorehabilitation Group, Department of Rehabilitation Sciences, Tervuursevest 101 Box 1501, 3000 Leuven, BelgiumPediatric Orthopedics, Department of Development and Regeneration, Herestraat 49 Box 7003, 3000 Leuven, BelgiumLaboratory of Respiratory Diseases and Thoracic Surgery, Department of Chronic Diseases and Metabolism, O&N 1bis Box 706, Herestraat 49, 3000 Leuven, BelgiumBotulinum toxin-A (BoNT-A) injection is known to exert beneficial effects on muscle tone, joint mobility and gait in children with cerebral palsy (CP). However, recent animal and human studies have raised the concern that BoNT-A might be harmful to muscle integrity. In CP-children, the impact of BoNT-A on muscle structure has been poorly studied, and inconsistent results have been reported. This study was aimed at determining the time course effect of a single BoNT-A administration on medial gastrocnemius (MG) morphology in CP-children. MG microbiopsies from 12 ambulant and BoNT-A-naïve CP-children (age, 3.4 (2.3) years, ranging from 2.5 to 7.8 years; seven boys and five girls; GMFCS I = 5, II = 4 and III = 3) were collected before and 3 and 6 months after BoNT-A treatment to analyze the fiber cross-sectional area (fCSA) and proportion; capillarization; and satellite cell (SC) content. Compared with the baseline, the fCSA decreased at 3 months (−14%, NS) and increased at 6 months (+13%, NS). Fiber size variability was significantly higher at 3 months (type I: +56%, <i>p</i> = 0.032; type IIa: +37%, <i>p</i> = 0.032) and 6 months (type I: +69%, <i>p</i> = 0.04; type IIa: +121%, <i>p</i> = 0.032) compared with the baseline. The higher type I proportion seen at 3 months was still present and more pronounced at 6 months (type I: +17%, <i>p</i> = 0.04; type IIx: −65%, <i>p</i> = 0.032). The capillary fiber density was reduced at 3 months (type I: −43%, NS; type II: −44%, <i>p</i> = 0.0320) but normalized at 6 months. There was a non-significant increase in SC/100 fibers at 3 months (+75%, NS) and 6 months (+40%, NS) compared with the baseline. These preliminary data suggest that BoNT-A induced alterations in the MG of children with CP, which were still present 6 months after BoNT-A injection but with signs of muscle recovery.https://www.mdpi.com/2072-6651/16/2/69microbiopsymyosin heavy chainspasticitymuscle fiber sizemuscle fiber proportionsatellite cells |
| spellingShingle | Jorieke Deschrevel Anke Andries Karen Maes Nathalie De Beukelaer Marlies Corvelyn Lauraine Staut Hannah De Houwer Domiziana Costamagna Kaat Desloovere Anja Van Campenhout Ghislaine Gayan-Ramirez Short-Term Effects of Botulinum Toxin-A Injection on the Medial Gastrocnemius Histological Features in Ambulant Children with Cerebral Palsy: A Longitudinal Pilot Study Toxins microbiopsy myosin heavy chain spasticity muscle fiber size muscle fiber proportion satellite cells |
| title | Short-Term Effects of Botulinum Toxin-A Injection on the Medial Gastrocnemius Histological Features in Ambulant Children with Cerebral Palsy: A Longitudinal Pilot Study |
| title_full | Short-Term Effects of Botulinum Toxin-A Injection on the Medial Gastrocnemius Histological Features in Ambulant Children with Cerebral Palsy: A Longitudinal Pilot Study |
| title_fullStr | Short-Term Effects of Botulinum Toxin-A Injection on the Medial Gastrocnemius Histological Features in Ambulant Children with Cerebral Palsy: A Longitudinal Pilot Study |
| title_full_unstemmed | Short-Term Effects of Botulinum Toxin-A Injection on the Medial Gastrocnemius Histological Features in Ambulant Children with Cerebral Palsy: A Longitudinal Pilot Study |
| title_short | Short-Term Effects of Botulinum Toxin-A Injection on the Medial Gastrocnemius Histological Features in Ambulant Children with Cerebral Palsy: A Longitudinal Pilot Study |
| title_sort | short term effects of botulinum toxin a injection on the medial gastrocnemius histological features in ambulant children with cerebral palsy a longitudinal pilot study |
| topic | microbiopsy myosin heavy chain spasticity muscle fiber size muscle fiber proportion satellite cells |
| url | https://www.mdpi.com/2072-6651/16/2/69 |
| work_keys_str_mv | AT joriekedeschrevel shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT ankeandries shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT karenmaes shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT nathaliedebeukelaer shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT marliescorvelyn shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT laurainestaut shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT hannahdehouwer shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT domizianacostamagna shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT kaatdesloovere shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT anjavancampenhout shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy AT ghislainegayanramirez shorttermeffectsofbotulinumtoxinainjectiononthemedialgastrocnemiushistologicalfeaturesinambulantchildrenwithcerebralpalsyalongitudinalpilotstudy |