Correlation of preferentially expressed antigen of melanoma (PRAME) gene expression with clinical characteristics in acute leukemia patients
Abstract Background Preferentially expressed antigen of melanoma (PRAME) gene is regularly overexpressed in acute leukemia (AL) and other malignant diseases which are recognized by human leucocyte antigen (HLA-24) located in the human chromosome of 22q11 coded by 509 amino acids. To rule out the PRA...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-022-00376-7 |
| _version_ | 1827279295882461184 |
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| author | Nagaraj V. Kulkarni Reshma A. Shetty Suchetha Kumari N Vijith V. Shetty Rajesh Krishna Meenakshi Arumugam Akanksha A. Kalal Prashanth Shetty |
| author_facet | Nagaraj V. Kulkarni Reshma A. Shetty Suchetha Kumari N Vijith V. Shetty Rajesh Krishna Meenakshi Arumugam Akanksha A. Kalal Prashanth Shetty |
| author_sort | Nagaraj V. Kulkarni |
| collection | DOAJ |
| description | Abstract Background Preferentially expressed antigen of melanoma (PRAME) gene is regularly overexpressed in acute leukemia (AL) and other malignant diseases which are recognized by human leucocyte antigen (HLA-24) located in the human chromosome of 22q11 coded by 509 amino acids. To rule out the PRAME gene expression in AL patients and its correlation with clinical characteristics in the Indian population set up by RT-qPCR. Results A total of 42 samples collected, 29 (69.4%) were males, and 13 (30.95%) were females, with a mean and standard deviation for age were 39.07 ± 22.22 years. Of which AML were of 22 (52.38%) cases, ALL were of 14 (33.33%) cases, and 6 (14.2%) cases which included other forms of leukemia. PRAME gene expression was highly expressed in thirty-three 27 (64.28%) AL patients compared to the least expression in healthy individuals. No significant difference between the different forms of AL (p=0.3203) was observed. Cytogenetic analysis of normal karyotype (NK), abnormal karyotype (Ab. K), and culture failure (CF) displayed statistical non-significance (p=0.5801). Among cytogenetic abnormalities obtained, no significant differences between the groups were observed (p=0.8507). Chloride, potassium, and absolute lymphocyte count (ALC) was found to be statistically significant with p=0.0038**, p=0.0358*, and p=0.0216*, respectively, between all other clinical characteristics. There was no correlation between the PRAME gene expression and clinical parameters. Conclusion PRAME gene expression in AL patients was highly expressed, comparable to studies reported globally with significant cytogenetic results. PRAME gene could be used as a potential diagnostic marker for monitoring the malignancies and minimal residual disease in AL. |
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| format | Article |
| id | doaj.art-f467bc0c77d44044819728618c260309 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:17:43Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
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| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-f467bc0c77d44044819728618c2603092024-04-17T03:22:48ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202022-07-012011810.1186/s43141-022-00376-7Correlation of preferentially expressed antigen of melanoma (PRAME) gene expression with clinical characteristics in acute leukemia patientsNagaraj V. Kulkarni0Reshma A. Shetty1Suchetha Kumari N2Vijith V. Shetty3Rajesh Krishna4Meenakshi Arumugam5Akanksha A. Kalal6Prashanth Shetty7Central Research Laboratory, KSHEMA Centre for Genetic Services, KS Hegde Medical Academy, NITTE (Deemed to be) UniversityCentral Research Laboratory, KSHEMA Centre for Genetic Services, KS Hegde Medical Academy, NITTE (Deemed to be) UniversityDepartment of Biochemistry, KS Hegde Medical Academy, NITTE (Deemed to be) UniversityDepartment of Medical Oncology, KS Hegde Medical Academy, NITTE (Deemed to be University)Yenepoya Medical College Hospital, Yenepoya (Deemed to be) UniversityCentral Research Laboratory, KSHEMA Centre for Genetic Services, KS Hegde Medical Academy, NITTE (Deemed to be) UniversityCentral Research Laboratory, KSHEMA Centre for Genetic Services, KS Hegde Medical Academy, NITTE (Deemed to be) UniversityCentral Research Laboratory, KSHEMA Centre for Genetic Services, KS Hegde Medical Academy, NITTE (Deemed to be) UniversityAbstract Background Preferentially expressed antigen of melanoma (PRAME) gene is regularly overexpressed in acute leukemia (AL) and other malignant diseases which are recognized by human leucocyte antigen (HLA-24) located in the human chromosome of 22q11 coded by 509 amino acids. To rule out the PRAME gene expression in AL patients and its correlation with clinical characteristics in the Indian population set up by RT-qPCR. Results A total of 42 samples collected, 29 (69.4%) were males, and 13 (30.95%) were females, with a mean and standard deviation for age were 39.07 ± 22.22 years. Of which AML were of 22 (52.38%) cases, ALL were of 14 (33.33%) cases, and 6 (14.2%) cases which included other forms of leukemia. PRAME gene expression was highly expressed in thirty-three 27 (64.28%) AL patients compared to the least expression in healthy individuals. No significant difference between the different forms of AL (p=0.3203) was observed. Cytogenetic analysis of normal karyotype (NK), abnormal karyotype (Ab. K), and culture failure (CF) displayed statistical non-significance (p=0.5801). Among cytogenetic abnormalities obtained, no significant differences between the groups were observed (p=0.8507). Chloride, potassium, and absolute lymphocyte count (ALC) was found to be statistically significant with p=0.0038**, p=0.0358*, and p=0.0216*, respectively, between all other clinical characteristics. There was no correlation between the PRAME gene expression and clinical parameters. Conclusion PRAME gene expression in AL patients was highly expressed, comparable to studies reported globally with significant cytogenetic results. PRAME gene could be used as a potential diagnostic marker for monitoring the malignancies and minimal residual disease in AL.https://doi.org/10.1186/s43141-022-00376-7Preferentially expressed antigen of melanoma (PRAME)Acute leukemiaCytogeneticsReverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) |
| spellingShingle | Nagaraj V. Kulkarni Reshma A. Shetty Suchetha Kumari N Vijith V. Shetty Rajesh Krishna Meenakshi Arumugam Akanksha A. Kalal Prashanth Shetty Correlation of preferentially expressed antigen of melanoma (PRAME) gene expression with clinical characteristics in acute leukemia patients Journal of Genetic Engineering and Biotechnology Preferentially expressed antigen of melanoma (PRAME) Acute leukemia Cytogenetics Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) |
| title | Correlation of preferentially expressed antigen of melanoma (PRAME) gene expression with clinical characteristics in acute leukemia patients |
| title_full | Correlation of preferentially expressed antigen of melanoma (PRAME) gene expression with clinical characteristics in acute leukemia patients |
| title_fullStr | Correlation of preferentially expressed antigen of melanoma (PRAME) gene expression with clinical characteristics in acute leukemia patients |
| title_full_unstemmed | Correlation of preferentially expressed antigen of melanoma (PRAME) gene expression with clinical characteristics in acute leukemia patients |
| title_short | Correlation of preferentially expressed antigen of melanoma (PRAME) gene expression with clinical characteristics in acute leukemia patients |
| title_sort | correlation of preferentially expressed antigen of melanoma prame gene expression with clinical characteristics in acute leukemia patients |
| topic | Preferentially expressed antigen of melanoma (PRAME) Acute leukemia Cytogenetics Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) |
| url | https://doi.org/10.1186/s43141-022-00376-7 |
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