Determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS
The bulbs of Lilium brownii Brown var. viridulum Baker (lily) are commonly known as “Bai-he” for both functional food and medicine in China. In this study, the combination of bio-assay guided fractionation and chemical analysis revealed that steroidal glycoside rich L. brownii fraction (SGL) exhibit...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464613002958 |
| _version_ | 1818691109013946368 |
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| author | Mengdi Zhu Jianguang Luo Huawei Lv Lingyi Kong |
| author_facet | Mengdi Zhu Jianguang Luo Huawei Lv Lingyi Kong |
| author_sort | Mengdi Zhu |
| collection | DOAJ |
| description | The bulbs of Lilium brownii Brown var. viridulum Baker (lily) are commonly known as “Bai-he” for both functional food and medicine in China. In this study, the combination of bio-assay guided fractionation and chemical analysis revealed that steroidal glycoside rich L. brownii fraction (SGL) exhibited potential anti-hyperglycaemic activity. Further investigations demonstrated that SGL could increase glucose consumption in HepG2 cells and 3T3-L1 adipocytes by 1.61- and 2.13-fold, respectively, and enhance 3T3-L1 preadipocyte differentiation. Total 29 steroidal glycosides in SGL were positively or tentatively identified using liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (LC-Q-TOF-MS/MS), which afforded us a comprehensive understanding of the bioactive fraction. These results suggested that steroidal glycosides in L. brownii possessed desirable potential in glucose metabolism and insulin sensitivity. This is the first report on the determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS. |
| first_indexed | 2024-12-17T12:36:40Z |
| format | Article |
| id | doaj.art-f4699ea6d20349b1ac5adb6230924ccf |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T12:36:40Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-f4699ea6d20349b1ac5adb6230924ccf2022-12-21T21:48:16ZengElsevierJournal of Functional Foods1756-46462014-01-016585597Determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MSMengdi Zhu0Jianguang Luo1Huawei Lv2Lingyi Kong3State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People’s Republic of ChinaState Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People’s Republic of ChinaState Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People’s Republic of ChinaCorresponding author. Tel./fax: +86 25 83271405.; State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People’s Republic of ChinaThe bulbs of Lilium brownii Brown var. viridulum Baker (lily) are commonly known as “Bai-he” for both functional food and medicine in China. In this study, the combination of bio-assay guided fractionation and chemical analysis revealed that steroidal glycoside rich L. brownii fraction (SGL) exhibited potential anti-hyperglycaemic activity. Further investigations demonstrated that SGL could increase glucose consumption in HepG2 cells and 3T3-L1 adipocytes by 1.61- and 2.13-fold, respectively, and enhance 3T3-L1 preadipocyte differentiation. Total 29 steroidal glycosides in SGL were positively or tentatively identified using liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (LC-Q-TOF-MS/MS), which afforded us a comprehensive understanding of the bioactive fraction. These results suggested that steroidal glycosides in L. brownii possessed desirable potential in glucose metabolism and insulin sensitivity. This is the first report on the determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS.http://www.sciencedirect.com/science/article/pii/S1756464613002958Lily bulbsAnti-hyperglycaemic activitySteroidal glycosidesLC-Q-TOF-MS/MS |
| spellingShingle | Mengdi Zhu Jianguang Luo Huawei Lv Lingyi Kong Determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS Journal of Functional Foods Lily bulbs Anti-hyperglycaemic activity Steroidal glycosides LC-Q-TOF-MS/MS |
| title | Determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS |
| title_full | Determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS |
| title_fullStr | Determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS |
| title_full_unstemmed | Determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS |
| title_short | Determination of anti-hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by LC-Q-TOF-MS/MS |
| title_sort | determination of anti hyperglycaemic activity in steroidal glycoside rich fraction of lily bulbs and characterization of the chemical profiles by lc q tof ms ms |
| topic | Lily bulbs Anti-hyperglycaemic activity Steroidal glycosides LC-Q-TOF-MS/MS |
| url | http://www.sciencedirect.com/science/article/pii/S1756464613002958 |
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