Application of Rhizobacteria, <i>Paraburkholderia fungorum</i> and <i>Delftia</i> sp. Confer Cadmium Tolerance in Rapeseed (<i>Brassica campestris</i>) through Modulating Antioxidant Defense and Glyoxalase Systems
We investigated the role of two different plant growth-promoting probiotic bacteria in conferring cadmium (Cd) tolerance in rapeseed (<i>Brassica campestris</i> cv. BARI Sarisha-14) through improving reactive oxygen species scavenging, antioxidant defense, and glyoxalase system. Soil, as...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/20/2738 |
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| author | Md. Rakib Hossain Raihan Mira Rahman Nur Uddin Mahmud Malay Kumar Adak Tofazzal Islam Masayuki Fujita Mirza Hasanuzzaman |
| author_facet | Md. Rakib Hossain Raihan Mira Rahman Nur Uddin Mahmud Malay Kumar Adak Tofazzal Islam Masayuki Fujita Mirza Hasanuzzaman |
| author_sort | Md. Rakib Hossain Raihan |
| collection | DOAJ |
| description | We investigated the role of two different plant growth-promoting probiotic bacteria in conferring cadmium (Cd) tolerance in rapeseed (<i>Brassica campestris</i> cv. BARI Sarisha-14) through improving reactive oxygen species scavenging, antioxidant defense, and glyoxalase system. Soil, as well as seeds of rapeseed, were separately treated with probiotic bacteria, <i>Paraburkholderia fungorum</i> BRRh-4 and <i>Delftia</i> sp. BTL-M2. Fourteen-day-old seedlings were exposed to 0.25 and 0.5 mM CdCl<sub>2</sub> for two weeks. Cadmium-treated plants resulted in a higher accumulation of hydrogen peroxide, increased lipid peroxidation, electrolyte leakage, chlorophyll damage, and impaired antioxidant defense and glyoxalase systems. Consequently, it reduced plant growth and biomass production, and yield parameters. However, probiotic bacteria-inoculated plants significantly ameliorated the Cd toxicity by enhancing the activities of antioxidant enzymes (ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase, glutathione reductase, glutathione peroxidase, and catalase) and glyoxalase enzymes (glyoxalase I and glyoxalase II) which led to the mitigation of oxidative damage indicated by reduced hydrogen peroxide, lipid peroxidation, and electrolyte leakage that ultimately improved growth, physiology, and yield of the bacterial inoculants rapeseed plants. When taken together, our results demonstrated the potential role of the plant probiotic bacteria, BRRh-4 and BTL-M2, in mitigating the Cd-induced damages in rapeseed plants. |
| first_indexed | 2024-03-09T19:34:08Z |
| format | Article |
| id | doaj.art-f469f98361b745eea700063a529001fc |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T19:34:08Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-f469f98361b745eea700063a529001fc2023-11-24T02:04:32ZengMDPI AGPlants2223-77472022-10-011120273810.3390/plants11202738Application of Rhizobacteria, <i>Paraburkholderia fungorum</i> and <i>Delftia</i> sp. Confer Cadmium Tolerance in Rapeseed (<i>Brassica campestris</i>) through Modulating Antioxidant Defense and Glyoxalase SystemsMd. Rakib Hossain Raihan0Mira Rahman1Nur Uddin Mahmud2Malay Kumar Adak3Tofazzal Islam4Masayuki Fujita5Mirza Hasanuzzaman6Department of Agronomy, Sher-e-Bangla Agricultural University, Dhaka 1207, BangladeshDepartment of Agronomy, Sher-e-Bangla Agricultural University, Dhaka 1207, BangladeshInstitute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshDepartment of Botany, University of Kalyani, Nadia 741235, West Bengal, IndiaInstitute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, BangladeshLaboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Takamatsu 761-0795, JapanDepartment of Agronomy, Sher-e-Bangla Agricultural University, Dhaka 1207, BangladeshWe investigated the role of two different plant growth-promoting probiotic bacteria in conferring cadmium (Cd) tolerance in rapeseed (<i>Brassica campestris</i> cv. BARI Sarisha-14) through improving reactive oxygen species scavenging, antioxidant defense, and glyoxalase system. Soil, as well as seeds of rapeseed, were separately treated with probiotic bacteria, <i>Paraburkholderia fungorum</i> BRRh-4 and <i>Delftia</i> sp. BTL-M2. Fourteen-day-old seedlings were exposed to 0.25 and 0.5 mM CdCl<sub>2</sub> for two weeks. Cadmium-treated plants resulted in a higher accumulation of hydrogen peroxide, increased lipid peroxidation, electrolyte leakage, chlorophyll damage, and impaired antioxidant defense and glyoxalase systems. Consequently, it reduced plant growth and biomass production, and yield parameters. However, probiotic bacteria-inoculated plants significantly ameliorated the Cd toxicity by enhancing the activities of antioxidant enzymes (ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase, glutathione reductase, glutathione peroxidase, and catalase) and glyoxalase enzymes (glyoxalase I and glyoxalase II) which led to the mitigation of oxidative damage indicated by reduced hydrogen peroxide, lipid peroxidation, and electrolyte leakage that ultimately improved growth, physiology, and yield of the bacterial inoculants rapeseed plants. When taken together, our results demonstrated the potential role of the plant probiotic bacteria, BRRh-4 and BTL-M2, in mitigating the Cd-induced damages in rapeseed plants.https://www.mdpi.com/2223-7747/11/20/2738abiotic stressmethylglyoxaloilseed cropplant-microbe interactionROSsoil heavy metals |
| spellingShingle | Md. Rakib Hossain Raihan Mira Rahman Nur Uddin Mahmud Malay Kumar Adak Tofazzal Islam Masayuki Fujita Mirza Hasanuzzaman Application of Rhizobacteria, <i>Paraburkholderia fungorum</i> and <i>Delftia</i> sp. Confer Cadmium Tolerance in Rapeseed (<i>Brassica campestris</i>) through Modulating Antioxidant Defense and Glyoxalase Systems Plants abiotic stress methylglyoxal oilseed crop plant-microbe interaction ROS soil heavy metals |
| title | Application of Rhizobacteria, <i>Paraburkholderia fungorum</i> and <i>Delftia</i> sp. Confer Cadmium Tolerance in Rapeseed (<i>Brassica campestris</i>) through Modulating Antioxidant Defense and Glyoxalase Systems |
| title_full | Application of Rhizobacteria, <i>Paraburkholderia fungorum</i> and <i>Delftia</i> sp. Confer Cadmium Tolerance in Rapeseed (<i>Brassica campestris</i>) through Modulating Antioxidant Defense and Glyoxalase Systems |
| title_fullStr | Application of Rhizobacteria, <i>Paraburkholderia fungorum</i> and <i>Delftia</i> sp. Confer Cadmium Tolerance in Rapeseed (<i>Brassica campestris</i>) through Modulating Antioxidant Defense and Glyoxalase Systems |
| title_full_unstemmed | Application of Rhizobacteria, <i>Paraburkholderia fungorum</i> and <i>Delftia</i> sp. Confer Cadmium Tolerance in Rapeseed (<i>Brassica campestris</i>) through Modulating Antioxidant Defense and Glyoxalase Systems |
| title_short | Application of Rhizobacteria, <i>Paraburkholderia fungorum</i> and <i>Delftia</i> sp. Confer Cadmium Tolerance in Rapeseed (<i>Brassica campestris</i>) through Modulating Antioxidant Defense and Glyoxalase Systems |
| title_sort | application of rhizobacteria i paraburkholderia fungorum i and i delftia i sp confer cadmium tolerance in rapeseed i brassica campestris i through modulating antioxidant defense and glyoxalase systems |
| topic | abiotic stress methylglyoxal oilseed crop plant-microbe interaction ROS soil heavy metals |
| url | https://www.mdpi.com/2223-7747/11/20/2738 |
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