Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery.
Vitreo-retinal (VR) surgeries induce conjunctival changes. However, there are no study reports regarding prevalence and severity of dry eye after these surgeries. This study evaluated dry eye outcome after VR surgery. Patients undergoing VR surgery classified as scleral buckle and microincision vitr...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0233517 |
| _version_ | 1818833521887674368 |
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| author | Ramalingam Mani P S Shobha Saravanan Thilagavathi Padmanabhan Prema Natarajan Viswanathan Ratra Vineet Ratra Dhanashree Narayanasamy Angayarkanni |
| author_facet | Ramalingam Mani P S Shobha Saravanan Thilagavathi Padmanabhan Prema Natarajan Viswanathan Ratra Vineet Ratra Dhanashree Narayanasamy Angayarkanni |
| author_sort | Ramalingam Mani |
| collection | DOAJ |
| description | Vitreo-retinal (VR) surgeries induce conjunctival changes. However, there are no study reports regarding prevalence and severity of dry eye after these surgeries. This study evaluated dry eye outcome after VR surgery. Patients undergoing VR surgery classified as scleral buckle and microincision vitrectomy surgery (n = 44, mean age: 56.09±10.2 years) were recruited. Dry eye evaluation was done before and 8 weeks after surgery (2 weeks after omitting topical eye drops). Conjunctival imprint cytology for goblet cell count and tear Mucin 5AC (MUC5AC) protein estimation was done. Gene expressions of MUC5AC, MUC4, MUC16, Aquaporin 4 (AQP4) and AQP5 were analyzed in the conjunctival imprint cells by qPCR. None of the patients exhibited clinical signs of dry eye after VR surgery. But the conjunctival goblet cell density (GCD) was significantly lowered post-VR surgery (63% cases, **p = 0.012) with no alterations in the tear MUC5AC protein. Post-VR surgery, the conjunctival cell gene expression of MUC4, MUC16 and AQP4 were significantly increased (*p = 0.025, *p = 0.05 and *p = 0.02 respectively) and AQP5 was significantly lowered (*p = 0.037), with no change in MUC5AC expression. Tear cytokines were significantly increased post-VR surgery (anti-inflammatory: IL1RA, IL4, IL5, IL9, FGF; PDGFbb and pro-inflammatory: IL2, IL6, IL15, GMCSF and IFNg). Though clinical signs of dry eye were not observed after VR surgery, ocular surface changes in the form of reduced GCD, altered MUC5AC, MUC4, MUC16, AQP4, AQP5 and cytokines are suggestive of dry eye outcome at the molecular level especially inpatients aged above 51 years, especially female gender and those who are diabetic. |
| first_indexed | 2024-12-19T02:20:15Z |
| format | Article |
| id | doaj.art-f46a3b843b3d46a49953b85f4a938e50 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T02:20:15Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f46a3b843b3d46a49953b85f4a938e502022-12-21T20:40:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01155e023351710.1371/journal.pone.0233517Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery.Ramalingam ManiP S ShobhaSaravanan ThilagavathiPadmanabhan PremaNatarajan ViswanathanRatra VineetRatra DhanashreeNarayanasamy AngayarkanniVitreo-retinal (VR) surgeries induce conjunctival changes. However, there are no study reports regarding prevalence and severity of dry eye after these surgeries. This study evaluated dry eye outcome after VR surgery. Patients undergoing VR surgery classified as scleral buckle and microincision vitrectomy surgery (n = 44, mean age: 56.09±10.2 years) were recruited. Dry eye evaluation was done before and 8 weeks after surgery (2 weeks after omitting topical eye drops). Conjunctival imprint cytology for goblet cell count and tear Mucin 5AC (MUC5AC) protein estimation was done. Gene expressions of MUC5AC, MUC4, MUC16, Aquaporin 4 (AQP4) and AQP5 were analyzed in the conjunctival imprint cells by qPCR. None of the patients exhibited clinical signs of dry eye after VR surgery. But the conjunctival goblet cell density (GCD) was significantly lowered post-VR surgery (63% cases, **p = 0.012) with no alterations in the tear MUC5AC protein. Post-VR surgery, the conjunctival cell gene expression of MUC4, MUC16 and AQP4 were significantly increased (*p = 0.025, *p = 0.05 and *p = 0.02 respectively) and AQP5 was significantly lowered (*p = 0.037), with no change in MUC5AC expression. Tear cytokines were significantly increased post-VR surgery (anti-inflammatory: IL1RA, IL4, IL5, IL9, FGF; PDGFbb and pro-inflammatory: IL2, IL6, IL15, GMCSF and IFNg). Though clinical signs of dry eye were not observed after VR surgery, ocular surface changes in the form of reduced GCD, altered MUC5AC, MUC4, MUC16, AQP4, AQP5 and cytokines are suggestive of dry eye outcome at the molecular level especially inpatients aged above 51 years, especially female gender and those who are diabetic.https://doi.org/10.1371/journal.pone.0233517 |
| spellingShingle | Ramalingam Mani P S Shobha Saravanan Thilagavathi Padmanabhan Prema Natarajan Viswanathan Ratra Vineet Ratra Dhanashree Narayanasamy Angayarkanni Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery. PLoS ONE |
| title | Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery. |
| title_full | Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery. |
| title_fullStr | Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery. |
| title_full_unstemmed | Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery. |
| title_short | Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery. |
| title_sort | altered mucins and aquaporins indicate dry eye outcome in patients undergoing vitreo retinal surgery |
| url | https://doi.org/10.1371/journal.pone.0233517 |
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