Optimization of Decolorization Process of Antarctic Krill Peptides by Response Surface Methodology

In order to optimize the decolorization process of Antarctic krill peptides and improve the product quality, activated carbon adsorption method was used to remove the pigment from the Antarctic krill peptides solution. Taking the decolorization rate and protein retention rate as the evaluation index...

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Main Authors: Xiaofang LIU, Yuelei HUANG, Kailiang LENG, Fuhou LI, Qi DING, Junkui MIAO, Yuan YU
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2022-12-01
Series:Shipin gongye ke-ji
Subjects:
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030136
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author Xiaofang LIU
Yuelei HUANG
Kailiang LENG
Fuhou LI
Qi DING
Junkui MIAO
Yuan YU
author_facet Xiaofang LIU
Yuelei HUANG
Kailiang LENG
Fuhou LI
Qi DING
Junkui MIAO
Yuan YU
author_sort Xiaofang LIU
collection DOAJ
description In order to optimize the decolorization process of Antarctic krill peptides and improve the product quality, activated carbon adsorption method was used to remove the pigment from the Antarctic krill peptides solution. Taking the decolorization rate and protein retention rate as the evaluation indexes, the influence of four independent variables including dosage of activated carbon, pH, decolorization temperature and decolorization time on the decolorization effect were investigated respectively. On the basis of single factor experiment, three independent variables including dosage of activated carbon, pH and decolorization time were optimized by the response surface methodology under the decolorization temperature of 50 ℃. Results showed that powdered activated carbon was more suitable for the removal of pigment from Antarctic krill peptides when compared with granular activated carbon, and the optimal conditions determined by response surface methodology were as follows: dosage of activated carbon 4.0%, pH1.5 and decolorization time 1.0 h. Under the optimal conditions, the decolorization rate was 82.19%±0.20% and the protein retention rate was 90.93%±2.28%. The decolorization treatment had no obvious effects on the proportion of essential amino acids and nonessential amino acids in the amino acid compositions as well as the molecular weight distribution of Antarctic krill peptides. This research would provide scientific support for the development of high-quality Antarctic krill peptides products.
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spelling doaj.art-f46af929b28448a5b008aa12009458e52022-12-22T04:39:33ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-12-01432321822510.13386/j.issn1002-0306.20220301362022030136-23Optimization of Decolorization Process of Antarctic Krill Peptides by Response Surface MethodologyXiaofang LIU0Yuelei HUANG1Kailiang LENG2Fuhou LI3Qi DING4Junkui MIAO5Yuan YU6Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, ChinaJiangsu Ocean University, School of Food Science and Engineering, Lianyungang 222005, ChinaQingdao Antarctic Weikang Biotechnology Co., Ltd., Qingdao 266112, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, ChinaIn order to optimize the decolorization process of Antarctic krill peptides and improve the product quality, activated carbon adsorption method was used to remove the pigment from the Antarctic krill peptides solution. Taking the decolorization rate and protein retention rate as the evaluation indexes, the influence of four independent variables including dosage of activated carbon, pH, decolorization temperature and decolorization time on the decolorization effect were investigated respectively. On the basis of single factor experiment, three independent variables including dosage of activated carbon, pH and decolorization time were optimized by the response surface methodology under the decolorization temperature of 50 ℃. Results showed that powdered activated carbon was more suitable for the removal of pigment from Antarctic krill peptides when compared with granular activated carbon, and the optimal conditions determined by response surface methodology were as follows: dosage of activated carbon 4.0%, pH1.5 and decolorization time 1.0 h. Under the optimal conditions, the decolorization rate was 82.19%±0.20% and the protein retention rate was 90.93%±2.28%. The decolorization treatment had no obvious effects on the proportion of essential amino acids and nonessential amino acids in the amino acid compositions as well as the molecular weight distribution of Antarctic krill peptides. This research would provide scientific support for the development of high-quality Antarctic krill peptides products.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030136antarctic krillpeptidesdecolorizationactivated carbonresponse surface methodology
spellingShingle Xiaofang LIU
Yuelei HUANG
Kailiang LENG
Fuhou LI
Qi DING
Junkui MIAO
Yuan YU
Optimization of Decolorization Process of Antarctic Krill Peptides by Response Surface Methodology
Shipin gongye ke-ji
antarctic krill
peptides
decolorization
activated carbon
response surface methodology
title Optimization of Decolorization Process of Antarctic Krill Peptides by Response Surface Methodology
title_full Optimization of Decolorization Process of Antarctic Krill Peptides by Response Surface Methodology
title_fullStr Optimization of Decolorization Process of Antarctic Krill Peptides by Response Surface Methodology
title_full_unstemmed Optimization of Decolorization Process of Antarctic Krill Peptides by Response Surface Methodology
title_short Optimization of Decolorization Process of Antarctic Krill Peptides by Response Surface Methodology
title_sort optimization of decolorization process of antarctic krill peptides by response surface methodology
topic antarctic krill
peptides
decolorization
activated carbon
response surface methodology
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030136
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AT fuhouli optimizationofdecolorizationprocessofantarctickrillpeptidesbyresponsesurfacemethodology
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