Actin in Action: Imaging Approaches to Study Cytoskeleton Structure and Function
The cytoskeleton plays several fundamental roles in the cell, including organizing the spatial arrangement of subcellular organelles, regulating cell dynamics and motility, providing a platform for interaction with neighboring cells, and ultimately defining overall cell shape. Fluorescence imaging h...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-11-01
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| Series: | Cells |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2073-4409/2/4/715 |
| _version_ | 1797701023172657152 |
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| author | Jeremy C. Simpson Katey K. McKayed |
| author_facet | Jeremy C. Simpson Katey K. McKayed |
| author_sort | Jeremy C. Simpson |
| collection | DOAJ |
| description | The cytoskeleton plays several fundamental roles in the cell, including organizing the spatial arrangement of subcellular organelles, regulating cell dynamics and motility, providing a platform for interaction with neighboring cells, and ultimately defining overall cell shape. Fluorescence imaging has proved to be vital in furthering our understanding of the cytoskeleton, and is now a mainstay technique used widely by cell biologists. In this review we provide an introduction to various imaging modalities used to study focal adhesions and the actin cytoskeleton, and using specific examples we highlight a number of recent studies in animal cells that have advanced our knowledge of cytoskeletal behavior. |
| first_indexed | 2024-03-12T04:29:21Z |
| format | Article |
| id | doaj.art-f46d5b662fdf451489e35b924ea54b97 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T04:29:21Z |
| publishDate | 2013-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-f46d5b662fdf451489e35b924ea54b972023-09-03T10:11:08ZengMDPI AGCells2073-44092013-11-012471573110.3390/cells2040715Actin in Action: Imaging Approaches to Study Cytoskeleton Structure and FunctionJeremy C. SimpsonKatey K. McKayedThe cytoskeleton plays several fundamental roles in the cell, including organizing the spatial arrangement of subcellular organelles, regulating cell dynamics and motility, providing a platform for interaction with neighboring cells, and ultimately defining overall cell shape. Fluorescence imaging has proved to be vital in furthering our understanding of the cytoskeleton, and is now a mainstay technique used widely by cell biologists. In this review we provide an introduction to various imaging modalities used to study focal adhesions and the actin cytoskeleton, and using specific examples we highlight a number of recent studies in animal cells that have advanced our knowledge of cytoskeletal behavior.http://www.mdpi.com/2073-4409/2/4/715cytoskeletonactinfocal adhesionsfluorescence imaginglive cell imaginghigh content screening and analysis |
| spellingShingle | Jeremy C. Simpson Katey K. McKayed Actin in Action: Imaging Approaches to Study Cytoskeleton Structure and Function Cells cytoskeleton actin focal adhesions fluorescence imaging live cell imaging high content screening and analysis |
| title | Actin in Action: Imaging Approaches to Study Cytoskeleton Structure and Function |
| title_full | Actin in Action: Imaging Approaches to Study Cytoskeleton Structure and Function |
| title_fullStr | Actin in Action: Imaging Approaches to Study Cytoskeleton Structure and Function |
| title_full_unstemmed | Actin in Action: Imaging Approaches to Study Cytoskeleton Structure and Function |
| title_short | Actin in Action: Imaging Approaches to Study Cytoskeleton Structure and Function |
| title_sort | actin in action imaging approaches to study cytoskeleton structure and function |
| topic | cytoskeleton actin focal adhesions fluorescence imaging live cell imaging high content screening and analysis |
| url | http://www.mdpi.com/2073-4409/2/4/715 |
| work_keys_str_mv | AT jeremycsimpson actininactionimagingapproachestostudycytoskeletonstructureandfunction AT kateykmckayed actininactionimagingapproachestostudycytoskeletonstructureandfunction |