Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles
Abstract Background Murraya paniculata (L.) Jack, commonly called orange jessamine in the family Rutaceae, is an important ornamental plant in tropical and subtropical regions which is famous for its strong fragrance. Although genome assemblies have been reported for many Rutaceae species, mainly in...
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| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Biology |
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| Online Access: | https://doi.org/10.1186/s12915-023-01639-6 |
| _version_ | 1797795551579734016 |
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| author | Tianyu Yang Xin Yin Haotong Kang Danni Yang Xingyu Yang Yunqiang Yang Yongping Yang |
| author_facet | Tianyu Yang Xin Yin Haotong Kang Danni Yang Xingyu Yang Yunqiang Yang Yongping Yang |
| author_sort | Tianyu Yang |
| collection | DOAJ |
| description | Abstract Background Murraya paniculata (L.) Jack, commonly called orange jessamine in the family Rutaceae, is an important ornamental plant in tropical and subtropical regions which is famous for its strong fragrance. Although genome assemblies have been reported for many Rutaceae species, mainly in the genus Citrus, full genomic information has not been reported for M. paniculata, which is a prerequisite for in-depth genetic studies on Murraya and manipulation using genetic engineering techniques. Here, we report a high-quality chromosome-level genome assembly of M. paniculata and aim to provide insights on the molecular mechanisms of flower volatile biosynthesis. Results The genome assembly with a contig N50 of 18.25 Mb consists of 9 pseudomolecules and has a total length of 216.86 Mb. Phylogenetic analysis revealed that M. paniculata diverged from the common ancestor approximately 25 million years ago and has not undergone any species-specific whole genome duplication events. Genome structural annotation and comparative genomics analysis revealed that there are obvious differences in transposon contents among the genomes of M. paniculata and Citrus species, especially in the upstream regions of genes. Research on the flower volatiles of M. paniculata and C. maxima at three flowering stages revealed significant differences in volatile composition with the flowers of C. maxima lacking benzaldehyde and phenylacetaldehyde. Notably, there are transposons inserted in the upstream region of the phenylacetaldehyde synthase (PAAS) genes Cg1g029630 and Cg1g029640 in C. maxima, but not in the upstream region of three PAAS genes Me2G_2379, Me2G_2381, and Me2G_2382 in M. paniculata. Our results indicated that compared to the low expression levels of PAAS genes in C. maxima, the higher expression levels of the three PAAS genes in M. paniculata are the main factor affecting the phenylacetaldehyde biosynthesis and causing the content difference of phenylacetaldehyde. The phenylacetaldehyde synthetic activities of the enzymes encoded by M. paniculata PAAS genes were validated by in vitro analyses. Conclusions Our study provides useful genomic resources of M. paniculata for further research on Rutaceae plants, identifies new PAAS genes, and provides insights into how transposons contribute to variations in flower volatiles among Murraya and Citrus plants. |
| first_indexed | 2024-03-13T03:19:40Z |
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| institution | Directory Open Access Journal |
| issn | 1741-7007 |
| language | English |
| last_indexed | 2024-03-13T03:19:40Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-f475a95af837417587c74418b24118ea2023-06-25T11:27:36ZengBMCBMC Biology1741-70072023-06-0121111310.1186/s12915-023-01639-6Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatilesTianyu Yang0Xin Yin1Haotong Kang2Danni Yang3Xingyu Yang4Yunqiang Yang5Yongping Yang6School of Life Science, Yunnan UniversityKey Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of ScienceKey Laboratory of Plant Resources Conservation and Utilization, College of Biological Resources and Environmental Sciences, Jishou UniversityKey Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of ScienceKey Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of ScienceKey Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of ScienceKey Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of ScienceAbstract Background Murraya paniculata (L.) Jack, commonly called orange jessamine in the family Rutaceae, is an important ornamental plant in tropical and subtropical regions which is famous for its strong fragrance. Although genome assemblies have been reported for many Rutaceae species, mainly in the genus Citrus, full genomic information has not been reported for M. paniculata, which is a prerequisite for in-depth genetic studies on Murraya and manipulation using genetic engineering techniques. Here, we report a high-quality chromosome-level genome assembly of M. paniculata and aim to provide insights on the molecular mechanisms of flower volatile biosynthesis. Results The genome assembly with a contig N50 of 18.25 Mb consists of 9 pseudomolecules and has a total length of 216.86 Mb. Phylogenetic analysis revealed that M. paniculata diverged from the common ancestor approximately 25 million years ago and has not undergone any species-specific whole genome duplication events. Genome structural annotation and comparative genomics analysis revealed that there are obvious differences in transposon contents among the genomes of M. paniculata and Citrus species, especially in the upstream regions of genes. Research on the flower volatiles of M. paniculata and C. maxima at three flowering stages revealed significant differences in volatile composition with the flowers of C. maxima lacking benzaldehyde and phenylacetaldehyde. Notably, there are transposons inserted in the upstream region of the phenylacetaldehyde synthase (PAAS) genes Cg1g029630 and Cg1g029640 in C. maxima, but not in the upstream region of three PAAS genes Me2G_2379, Me2G_2381, and Me2G_2382 in M. paniculata. Our results indicated that compared to the low expression levels of PAAS genes in C. maxima, the higher expression levels of the three PAAS genes in M. paniculata are the main factor affecting the phenylacetaldehyde biosynthesis and causing the content difference of phenylacetaldehyde. The phenylacetaldehyde synthetic activities of the enzymes encoded by M. paniculata PAAS genes were validated by in vitro analyses. Conclusions Our study provides useful genomic resources of M. paniculata for further research on Rutaceae plants, identifies new PAAS genes, and provides insights into how transposons contribute to variations in flower volatiles among Murraya and Citrus plants.https://doi.org/10.1186/s12915-023-01639-6Murraya paniculataCitrus speciesComparative genomicsTransposonFlower volatilesPhenylacetaldehyde |
| spellingShingle | Tianyu Yang Xin Yin Haotong Kang Danni Yang Xingyu Yang Yunqiang Yang Yongping Yang Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles BMC Biology Murraya paniculata Citrus species Comparative genomics Transposon Flower volatiles Phenylacetaldehyde |
| title | Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles |
| title_full | Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles |
| title_fullStr | Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles |
| title_full_unstemmed | Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles |
| title_short | Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles |
| title_sort | chromosome level genome assembly of murraya paniculata sheds light on biosynthesis of floral volatiles |
| topic | Murraya paniculata Citrus species Comparative genomics Transposon Flower volatiles Phenylacetaldehyde |
| url | https://doi.org/10.1186/s12915-023-01639-6 |
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