Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles
A sweet solution for studying cacao genetics Genomic insights from an efficient, low-cost DNA sequencing strategy could enable more sustainable cacao cultivation. Growing demand for chocolate is currently being satisfied by increasing land use. A richer understanding of this plant’s genome could ena...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2019-04-01
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| Series: | Horticulture Research |
| Online Access: | https://doi.org/10.1038/s41438-019-0125-7 |
| _version_ | 1828469522548391936 |
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| author | Joe Morrissey J. Conrad Stack Rebecca Valls Juan Carlos Motamayor |
| author_facet | Joe Morrissey J. Conrad Stack Rebecca Valls Juan Carlos Motamayor |
| author_sort | Joe Morrissey |
| collection | DOAJ |
| description | A sweet solution for studying cacao genetics Genomic insights from an efficient, low-cost DNA sequencing strategy could enable more sustainable cacao cultivation. Growing demand for chocolate is currently being satisfied by increasing land use. A richer understanding of this plant’s genome could enable development of more productive strains, and Juan Carlos Motamayor and colleagues at Mars Chocolate have demonstrated a method for rapidly collecting such data. Most sequencing platforms produce vast multitudes of short DNA sequences which must then be assembled into a final map—a particular challenge for cacao, whose genome contains complex repetitive regions that are tough to reconstruct. Using a newer platform known as MinION, which produces far longer sequence reads, Motamayor’s team assembled a detailed genome map for a widely-used cacao cultivar within months for under $5,000. This approach could facilitate genetic engineering efforts to improve crop performance. |
| first_indexed | 2024-12-11T04:40:35Z |
| format | Article |
| id | doaj.art-f475d725bad149459e407d58948066b1 |
| institution | Directory Open Access Journal |
| issn | 2052-7276 |
| language | English |
| last_indexed | 2024-12-11T04:40:35Z |
| publishDate | 2019-04-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Horticulture Research |
| spelling | doaj.art-f475d725bad149459e407d58948066b12022-12-22T01:20:39ZengOxford University PressHorticulture Research2052-72762019-04-016111310.1038/s41438-019-0125-7Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubblesJoe Morrissey0J. Conrad Stack1Rebecca Valls2Juan Carlos Motamayor3Mars ChocolateMars ChocolateMars ChocolateMars ChocolateA sweet solution for studying cacao genetics Genomic insights from an efficient, low-cost DNA sequencing strategy could enable more sustainable cacao cultivation. Growing demand for chocolate is currently being satisfied by increasing land use. A richer understanding of this plant’s genome could enable development of more productive strains, and Juan Carlos Motamayor and colleagues at Mars Chocolate have demonstrated a method for rapidly collecting such data. Most sequencing platforms produce vast multitudes of short DNA sequences which must then be assembled into a final map—a particular challenge for cacao, whose genome contains complex repetitive regions that are tough to reconstruct. Using a newer platform known as MinION, which produces far longer sequence reads, Motamayor’s team assembled a detailed genome map for a widely-used cacao cultivar within months for under $5,000. This approach could facilitate genetic engineering efforts to improve crop performance.https://doi.org/10.1038/s41438-019-0125-7 |
| spellingShingle | Joe Morrissey J. Conrad Stack Rebecca Valls Juan Carlos Motamayor Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles Horticulture Research |
| title | Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles |
| title_full | Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles |
| title_fullStr | Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles |
| title_full_unstemmed | Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles |
| title_short | Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles |
| title_sort | low cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles |
| url | https://doi.org/10.1038/s41438-019-0125-7 |
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