Retracted: Long non‐coding RNA PGM5‐AS1 promotes epithelial‐mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR‐140‐5p‐mediated FBN1 inhibition
Osteosarcoma is an uncommon tumor occurring in bone, accompanied by elevated incidence and reduced rate of healing. Epithelial‐to‐mesenchymal transition (EMT) serves as a conceptual paradigm to explain the invasion and metastasis of osteosarcoma and other cancers. Hence, developing effective therape...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-10-01
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| Series: | Molecular Oncology |
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| Online Access: | https://doi.org/10.1002/1878-0261.12711 |
| _version_ | 1797776349026320384 |
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| author | Wei Liu Pengcheng Liu Hang Gao Xu Wang Ming Yan |
| author_facet | Wei Liu Pengcheng Liu Hang Gao Xu Wang Ming Yan |
| author_sort | Wei Liu |
| collection | DOAJ |
| description | Osteosarcoma is an uncommon tumor occurring in bone, accompanied by elevated incidence and reduced rate of healing. Epithelial‐to‐mesenchymal transition (EMT) serves as a conceptual paradigm to explain the invasion and metastasis of osteosarcoma and other cancers. Hence, developing effective therapeutic strategy to treat the EMT of osteosarcoma is essential. Here, we identified the molecular mechanism of long noncoding RNA (lncRNA) PGM5‐AS1 in EMT and progression of osteosarcoma. Microarray‐based analysis was employed to screen the osteosarcoma‐related differentially expressed lncRNAs. The levels of PGM5‐AS1 as well as microRNA‐140‐5p (miR‐140‐5p) and fibrillin‐1 (FBN1) in osteosarcoma tissues and cells were determined. Dual‐luciferase reporter gene assay, RNA pull‐down assay, and RNA immunoprecipitation assay were conducted to validate the relationship among PGM5‐AS1, miR‐140‐5p, and FBN1. Expression of PGM5‐AS1, miR‐140‐5p, and FBN1 was altered by overexpression, shRNA, mimic, or inhibitors in order to investigate how they regulated migration, invasion, and EMT of osteosarcoma cells in vitro. Loss‐ and gain‐of‐function approaches were employed in nude mice to detect their roles in tumorigenesis in vivo. Osteosarcoma tissues and cells exhibited low expression of miR‐140‐5p, but high expression of PGM5‐AS1 and FBN1. PGM5‐AS1 competitively bound to miR‐140‐5p to upregulate FBN1. Furthermore, hindering PGM5‐AS1 and FBN1 or overexpressing miR‐140‐5p dampened migration, invasion, and EMT of osteosarcoma cells in vitro. Furthermore, silencing PGM5‐AS1 or FBN1, or overexpressing miR‐140‐5p markedly inhibited tumorigenesis in nude mice in vivo. Taken together, PGM5‐AS1 depletion causes FBN1 reduction to retard osteosarcoma processes by negatively modulating miR‐140‐5p. |
| first_indexed | 2024-03-12T22:49:37Z |
| format | Article |
| id | doaj.art-f475db646a064d69b752e41867c21787 |
| institution | Directory Open Access Journal |
| issn | 1574-7891 1878-0261 |
| language | English |
| last_indexed | 2024-03-12T22:49:37Z |
| publishDate | 2020-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Oncology |
| spelling | doaj.art-f475db646a064d69b752e41867c217872023-07-20T17:20:38ZengWileyMolecular Oncology1574-78911878-02612020-10-0114102660267710.1002/1878-0261.12711Retracted: Long non‐coding RNA PGM5‐AS1 promotes epithelial‐mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR‐140‐5p‐mediated FBN1 inhibitionWei Liu0Pengcheng Liu1Hang Gao2Xu Wang3Ming Yan4Department of Spine Surgery The First Hospital of Jilin University Changchun ChinaDepartment of Hand and Foot Surgery The First Hospital of Jilin University Changchun ChinaDepartment of Bone and Joint Surgery The First Hospital of Jilin University Changchun ChinaDepartment of Colorectal and Anal Surgery The First Hospital of Jilin University Changchun ChinaDepartment of Spine Surgery The First Hospital of Jilin University Changchun ChinaOsteosarcoma is an uncommon tumor occurring in bone, accompanied by elevated incidence and reduced rate of healing. Epithelial‐to‐mesenchymal transition (EMT) serves as a conceptual paradigm to explain the invasion and metastasis of osteosarcoma and other cancers. Hence, developing effective therapeutic strategy to treat the EMT of osteosarcoma is essential. Here, we identified the molecular mechanism of long noncoding RNA (lncRNA) PGM5‐AS1 in EMT and progression of osteosarcoma. Microarray‐based analysis was employed to screen the osteosarcoma‐related differentially expressed lncRNAs. The levels of PGM5‐AS1 as well as microRNA‐140‐5p (miR‐140‐5p) and fibrillin‐1 (FBN1) in osteosarcoma tissues and cells were determined. Dual‐luciferase reporter gene assay, RNA pull‐down assay, and RNA immunoprecipitation assay were conducted to validate the relationship among PGM5‐AS1, miR‐140‐5p, and FBN1. Expression of PGM5‐AS1, miR‐140‐5p, and FBN1 was altered by overexpression, shRNA, mimic, or inhibitors in order to investigate how they regulated migration, invasion, and EMT of osteosarcoma cells in vitro. Loss‐ and gain‐of‐function approaches were employed in nude mice to detect their roles in tumorigenesis in vivo. Osteosarcoma tissues and cells exhibited low expression of miR‐140‐5p, but high expression of PGM5‐AS1 and FBN1. PGM5‐AS1 competitively bound to miR‐140‐5p to upregulate FBN1. Furthermore, hindering PGM5‐AS1 and FBN1 or overexpressing miR‐140‐5p dampened migration, invasion, and EMT of osteosarcoma cells in vitro. Furthermore, silencing PGM5‐AS1 or FBN1, or overexpressing miR‐140‐5p markedly inhibited tumorigenesis in nude mice in vivo. Taken together, PGM5‐AS1 depletion causes FBN1 reduction to retard osteosarcoma processes by negatively modulating miR‐140‐5p.https://doi.org/10.1002/1878-0261.12711fibrillin‐1long noncoding RNA PGM5‐AS1microRNA‐140‐5posteosarcoma |
| spellingShingle | Wei Liu Pengcheng Liu Hang Gao Xu Wang Ming Yan Retracted: Long non‐coding RNA PGM5‐AS1 promotes epithelial‐mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR‐140‐5p‐mediated FBN1 inhibition Molecular Oncology fibrillin‐1 long noncoding RNA PGM5‐AS1 microRNA‐140‐5p osteosarcoma |
| title | Retracted: Long non‐coding RNA PGM5‐AS1 promotes epithelial‐mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR‐140‐5p‐mediated FBN1 inhibition |
| title_full | Retracted: Long non‐coding RNA PGM5‐AS1 promotes epithelial‐mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR‐140‐5p‐mediated FBN1 inhibition |
| title_fullStr | Retracted: Long non‐coding RNA PGM5‐AS1 promotes epithelial‐mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR‐140‐5p‐mediated FBN1 inhibition |
| title_full_unstemmed | Retracted: Long non‐coding RNA PGM5‐AS1 promotes epithelial‐mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR‐140‐5p‐mediated FBN1 inhibition |
| title_short | Retracted: Long non‐coding RNA PGM5‐AS1 promotes epithelial‐mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR‐140‐5p‐mediated FBN1 inhibition |
| title_sort | retracted long non coding rna pgm5 as1 promotes epithelial mesenchymal transition invasion and metastasis of osteosarcoma cells by impairing mir 140 5p mediated fbn1 inhibition |
| topic | fibrillin‐1 long noncoding RNA PGM5‐AS1 microRNA‐140‐5p osteosarcoma |
| url | https://doi.org/10.1002/1878-0261.12711 |
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