Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach
The microbiology of caves is an important topic for better understanding subsurface biosphere diversity. The diversity and taxonomic composition of bacterial communities associated with cave walls of the Oylat Cave was studied first time by molecular cloning basedon Sanger/pyrosequencing approach. R...
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| Format: | Article |
| Language: | English |
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Sciendo
2016-03-01
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| Series: | Polish Journal of Microbiology |
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| Online Access: | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/1/pdf/10.5604_17331331.1197277.pdf |
| _version_ | 1828445144549949440 |
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| author | Yasemin Gulecal-Pektas |
| author_facet | Yasemin Gulecal-Pektas |
| author_sort | Yasemin Gulecal-Pektas |
| collection | DOAJ |
| description | The microbiology of caves is an important topic for better understanding subsurface biosphere diversity. The diversity and taxonomic composition of bacterial communities associated with cave walls of the Oylat Cave was studied first time by molecular cloning basedon Sanger/pyrosequencing approach. Results showed an average of 1,822 operational taxonomic units per sample. Clones analyzed from Oylat Cave were found to belong to 10 common phyla within the domain Bacteria. Proteobacteria dominated the phyla, followed by Actinobacteria, Acidobacteria and Nitrospirae. Shannon diversity index was between to 3.76 and 5.35. The robust analysis conducted for this study demonstrated high bacterial diversity on cave rock wall surfaces. |
| first_indexed | 2024-12-10T21:53:20Z |
| format | Article |
| id | doaj.art-f47635c9a34a44399ca50d300c4e269b |
| institution | Directory Open Access Journal |
| issn | 1733-1331 2544-4646 |
| language | English |
| last_indexed | 2024-12-10T21:53:20Z |
| publishDate | 2016-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-f47635c9a34a44399ca50d300c4e269b2022-12-22T01:32:07ZengSciendoPolish Journal of Microbiology1733-13312544-46462016-03-0165110.5604/17331331.1197277Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing ApproachYasemin Gulecal-Pektas0Institute of Science and Engineering, University of Istanbul, Istanbul, TurkeyThe microbiology of caves is an important topic for better understanding subsurface biosphere diversity. The diversity and taxonomic composition of bacterial communities associated with cave walls of the Oylat Cave was studied first time by molecular cloning basedon Sanger/pyrosequencing approach. Results showed an average of 1,822 operational taxonomic units per sample. Clones analyzed from Oylat Cave were found to belong to 10 common phyla within the domain Bacteria. Proteobacteria dominated the phyla, followed by Actinobacteria, Acidobacteria and Nitrospirae. Shannon diversity index was between to 3.76 and 5.35. The robust analysis conducted for this study demonstrated high bacterial diversity on cave rock wall surfaces.https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/1/pdf/10.5604_17331331.1197277.pdfbacterial diversityOylat CaveSanger/pyrosequencingsubsurface biosphere |
| spellingShingle | Yasemin Gulecal-Pektas Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach Polish Journal of Microbiology bacterial diversity Oylat Cave Sanger/pyrosequencing subsurface biosphere |
| title | Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach |
| title_full | Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach |
| title_fullStr | Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach |
| title_full_unstemmed | Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach |
| title_short | Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach |
| title_sort | bacterial diversity and composition in oylat cave turkey with combined sanger pyrosequencing approach |
| topic | bacterial diversity Oylat Cave Sanger/pyrosequencing subsurface biosphere |
| url | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/1/pdf/10.5604_17331331.1197277.pdf |
| work_keys_str_mv | AT yasemingulecalpektas bacterialdiversityandcompositioninoylatcaveturkeywithcombinedsangerpyrosequencingapproach |