Stimulation of risogenesis of red and black raspberry in vitro with use of coherent radiation
The effect of supplementary red LED and laser lightning on the rhizogenesis in vitro of plants of the genus Rubus at different levels of the basic polychromatic illumination was studied. The relevance of this work is due to the need to improve the biotechnological methods of accelerated plant propag...
| Main Authors: | , |
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| Format: | Article |
| Language: | Russian |
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Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
2018-10-01
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| Series: | Аграрная наука Евро-Северо-Востока |
| Subjects: | |
| Online Access: | https://www.agronauka-sv.ru/jour/article/view/256 |
| _version_ | 1797290306648932352 |
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| author | N. V. Solovykh A. V. Budagovsky |
| author_facet | N. V. Solovykh A. V. Budagovsky |
| author_sort | N. V. Solovykh |
| collection | DOAJ |
| description | The effect of supplementary red LED and laser lightning on the rhizogenesis in vitro of plants of the genus Rubus at different levels of the basic polychromatic illumination was studied. The relevance of this work is due to the need to improve the biotechnological methods of accelerated plant propagation at all stages of cloning, including root formation stage. One of the ways to solve this problem is to use coherent radiation to stimulate physiological and morphogenetic processes in plants and to create energy-saving cultivation technologies on the basis of the methods developed. Microcuttings of berry crops cultivated with 1000 and 2000 Lux illumination were subjected to daily supplementary lightning for 20 minutes with LEDs (λ = 638 nm, power density 5 W/m2) or helium-neon laser (λ = 632.8 nm, power density 3 W/m2). In conditions of low illumination (1000 Lux), the intensity of rhizogenesis in red raspberry and black raspberry decreased in comparison with the illumination of 2000 Lux. LED-based and laser additional irradiation increased the intensity of the rhizogenesis of berry crops in vitro. With a low basic illumination (1000 Lux) the maximum stimulating effect of an additional red quasi-monochromatic illumination was observed. In red and black raspberry, laser and LED backlighting with insufficient basic illumination made it possible to obtain the number of roots and their total length per one explant not differing statistically from those at full illumination, but without backlighting. |
| first_indexed | 2024-03-07T19:20:31Z |
| format | Article |
| id | doaj.art-f478776875c94ac88010c9ec6c16c6be |
| institution | Directory Open Access Journal |
| issn | 2072-9081 2500-1396 |
| language | Russian |
| last_indexed | 2024-03-07T19:20:31Z |
| publishDate | 2018-10-01 |
| publisher | Federal Agricultural Research Center of the North-East named N.V. Rudnitsky |
| record_format | Article |
| series | Аграрная наука Евро-Северо-Востока |
| spelling | doaj.art-f478776875c94ac88010c9ec6c16c6be2024-02-29T11:31:11ZrusFederal Agricultural Research Center of the North-East named N.V. RudnitskyАграрная наука Евро-Северо-Востока2072-90812500-13962018-10-01665646810.30766/2072-9081.2018.66.5.64-68255Stimulation of risogenesis of red and black raspberry in vitro with use of coherent radiationN. V. Solovykh0A. V. Budagovsky1I. V. Michurin Federal Scientific centerI. V. Michurin Federal Scientific centerThe effect of supplementary red LED and laser lightning on the rhizogenesis in vitro of plants of the genus Rubus at different levels of the basic polychromatic illumination was studied. The relevance of this work is due to the need to improve the biotechnological methods of accelerated plant propagation at all stages of cloning, including root formation stage. One of the ways to solve this problem is to use coherent radiation to stimulate physiological and morphogenetic processes in plants and to create energy-saving cultivation technologies on the basis of the methods developed. Microcuttings of berry crops cultivated with 1000 and 2000 Lux illumination were subjected to daily supplementary lightning for 20 minutes with LEDs (λ = 638 nm, power density 5 W/m2) or helium-neon laser (λ = 632.8 nm, power density 3 W/m2). In conditions of low illumination (1000 Lux), the intensity of rhizogenesis in red raspberry and black raspberry decreased in comparison with the illumination of 2000 Lux. LED-based and laser additional irradiation increased the intensity of the rhizogenesis of berry crops in vitro. With a low basic illumination (1000 Lux) the maximum stimulating effect of an additional red quasi-monochromatic illumination was observed. In red and black raspberry, laser and LED backlighting with insufficient basic illumination made it possible to obtain the number of roots and their total length per one explant not differing statistically from those at full illumination, but without backlighting.https://www.agronauka-sv.ru/jour/article/view/256clonal micropropagation in vitrorhizogenesisphotostimulationsystem of phytochromesred raspberryblack raspberry |
| spellingShingle | N. V. Solovykh A. V. Budagovsky Stimulation of risogenesis of red and black raspberry in vitro with use of coherent radiation Аграрная наука Евро-Северо-Востока clonal micropropagation in vitro rhizogenesis photostimulation system of phytochromes red raspberry black raspberry |
| title | Stimulation of risogenesis of red and black raspberry in vitro with use of coherent radiation |
| title_full | Stimulation of risogenesis of red and black raspberry in vitro with use of coherent radiation |
| title_fullStr | Stimulation of risogenesis of red and black raspberry in vitro with use of coherent radiation |
| title_full_unstemmed | Stimulation of risogenesis of red and black raspberry in vitro with use of coherent radiation |
| title_short | Stimulation of risogenesis of red and black raspberry in vitro with use of coherent radiation |
| title_sort | stimulation of risogenesis of red and black raspberry in vitro with use of coherent radiation |
| topic | clonal micropropagation in vitro rhizogenesis photostimulation system of phytochromes red raspberry black raspberry |
| url | https://www.agronauka-sv.ru/jour/article/view/256 |
| work_keys_str_mv | AT nvsolovykh stimulationofrisogenesisofredandblackraspberryinvitrowithuseofcoherentradiation AT avbudagovsky stimulationofrisogenesisofredandblackraspberryinvitrowithuseofcoherentradiation |