Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey
Abstract The determination of hydroxymethylfurfural (HMF) in bee honey is performed by the spectrophotometric methods Winkler's and White's. The Winkler method reads the absorbances in the visible region of the spectrum, and the White method measures in the ultraviolet (UV) and visible reg...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia de Alimentos (ITAL)
2023-05-01
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| Series: | Brazilian Journal of Food Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232023000100415&lng=en&tlng=en |
| _version_ | 1797816907011719168 |
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| author | Isabel Cabral Maeda Aryele Nunes da Cruz Encide Sampaio Evelyn Fernanda Flores Caron José Fernando Nardy Sarah Cristina de Oliveira Juliano Gonçalves Pereira Otávio Augusto Martins |
| author_facet | Isabel Cabral Maeda Aryele Nunes da Cruz Encide Sampaio Evelyn Fernanda Flores Caron José Fernando Nardy Sarah Cristina de Oliveira Juliano Gonçalves Pereira Otávio Augusto Martins |
| author_sort | Isabel Cabral Maeda |
| collection | DOAJ |
| description | Abstract The determination of hydroxymethylfurfural (HMF) in bee honey is performed by the spectrophotometric methods Winkler's and White's. The Winkler method reads the absorbances in the visible region of the spectrum, and the White method measures in the ultraviolet (UV) and visible regions of the spectrum. This study aims to compare the spectrophotometric methodologies of White and Winkler to the determination of HMF in bee honey. For the study, it was used a UV-visible spectrophotometer, 10 mm optical path quartz cuvettes, and ten bee honey samples of different flowering. Absorbances at 550 nm were analyzed by the Winkler method; absorbances at 284 nm and 336 nm in the White method; and a comparison of HMF concentration (mg/kg) was carried out between the methods. The results showed significant differences (p<0.05) in HMF concentration determined by both methods. The main conclusion is that the Winkler method presents higher HMF values than the White method. |
| first_indexed | 2024-03-13T08:44:37Z |
| format | Article |
| id | doaj.art-f47a6f62305e4d49a34c90285d820da7 |
| institution | Directory Open Access Journal |
| issn | 1981-6723 |
| language | English |
| last_indexed | 2024-03-13T08:44:37Z |
| publishDate | 2023-05-01 |
| publisher | Instituto de Tecnologia de Alimentos (ITAL) |
| record_format | Article |
| series | Brazilian Journal of Food Technology |
| spelling | doaj.art-f47a6f62305e4d49a34c90285d820da72023-05-30T07:33:54ZengInstituto de Tecnologia de Alimentos (ITAL)Brazilian Journal of Food Technology1981-67232023-05-012610.1590/1981-6723.13322Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honeyIsabel Cabral MaedaAryele Nunes da Cruz Encide Sampaiohttps://orcid.org/0000-0003-3648-2382Evelyn Fernanda Flores CaronJosé Fernando NardySarah Cristina de OliveiraJuliano Gonçalves Pereirahttps://orcid.org/0000-0002-8713-7506Otávio Augusto MartinsAbstract The determination of hydroxymethylfurfural (HMF) in bee honey is performed by the spectrophotometric methods Winkler's and White's. The Winkler method reads the absorbances in the visible region of the spectrum, and the White method measures in the ultraviolet (UV) and visible regions of the spectrum. This study aims to compare the spectrophotometric methodologies of White and Winkler to the determination of HMF in bee honey. For the study, it was used a UV-visible spectrophotometer, 10 mm optical path quartz cuvettes, and ten bee honey samples of different flowering. Absorbances at 550 nm were analyzed by the Winkler method; absorbances at 284 nm and 336 nm in the White method; and a comparison of HMF concentration (mg/kg) was carried out between the methods. The results showed significant differences (p<0.05) in HMF concentration determined by both methods. The main conclusion is that the Winkler method presents higher HMF values than the White method.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232023000100415&lng=en&tlng=enAbsorbanceBeesComparisonHMFPhysicochemicalSpectrophotometer |
| spellingShingle | Isabel Cabral Maeda Aryele Nunes da Cruz Encide Sampaio Evelyn Fernanda Flores Caron José Fernando Nardy Sarah Cristina de Oliveira Juliano Gonçalves Pereira Otávio Augusto Martins Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey Brazilian Journal of Food Technology Absorbance Bees Comparison HMF Physicochemical Spectrophotometer |
| title | Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey |
| title_full | Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey |
| title_fullStr | Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey |
| title_full_unstemmed | Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey |
| title_short | Spectrophotometry of Winkler and White's official methods for the determination of hydroxymethylfurfural in bee honey |
| title_sort | spectrophotometry of winkler and white s official methods for the determination of hydroxymethylfurfural in bee honey |
| topic | Absorbance Bees Comparison HMF Physicochemical Spectrophotometer |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232023000100415&lng=en&tlng=en |
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