In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells.
Stem cells are of great interest for regenerating disturbed tissues and organs. These cells are commonly isolated from the bone marrow, but there has been interest in other tissues in the recent years. In this study, we evaluated the possibility of isolation of stem cells from oral connective tissue...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2012-03-01
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| Series: | Frontiers in Dentistry |
| Subjects: | |
| Online Access: | https://jdt.tums.ac.ir/index.php/jdt/article/view/792 |
| _version_ | 1811289075316948992 |
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| author | P Torkzaban A Saffarpour M Bidgoli S Sohilifar |
| author_facet | P Torkzaban A Saffarpour M Bidgoli S Sohilifar |
| author_sort | P Torkzaban |
| collection | DOAJ |
| description | Stem cells are of great interest for regenerating disturbed tissues and organs. These cells are commonly isolated from the bone marrow, but there has been interest in other tissues in the recent years. In this study, we evaluated the possibility of isolation of stem cells from oral connective tissue and investigated their characteristics.In this experimental study, sampling from the bone marrow and oral connective tissue of a beagle dog was performed under general anesthesia. Bone marrow stem cell isolation was performed according to the established protocols. The samples obtained from oral soft tissue were broken to small pieces and after adding collagenase I, the samples were incubated for 45 minutes in 37°C. Other processes were similar to the processes which were carried out on bone marrow cells. Then cell properties were compared to evaluate if the cells from the connective tissue were stem cells.The cells from the bone marrow and connective tissue had the same morphology. The result of colony forming unit assay was relatively similar. Population doubling time was similar too. In addition, both cell groups differentiated to osteoblasts in osteogenic media.The cells isolated from the oral connective tissue had the characteristics of stem cells, including fibroblastoid morphology, self renewal properties, high proliferation rate and differentiation potential. |
| first_indexed | 2024-04-13T03:48:33Z |
| format | Article |
| id | doaj.art-f47b47ac72b148f1849ad2ec9c262544 |
| institution | Directory Open Access Journal |
| issn | 2676-296X |
| language | English |
| last_indexed | 2024-04-13T03:48:33Z |
| publishDate | 2012-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Frontiers in Dentistry |
| spelling | doaj.art-f47b47ac72b148f1849ad2ec9c2625442022-12-22T03:03:55ZengTehran University of Medical SciencesFrontiers in Dentistry2676-296X2012-03-0191In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells.P Torkzaban0A Saffarpour1M Bidgoli2S Sohilifar3Periodontology Department, Dental Research Center, Faculty of Dentistry, Hamedan University of medical science, Hamedan, Iran. Stem cells are of great interest for regenerating disturbed tissues and organs. These cells are commonly isolated from the bone marrow, but there has been interest in other tissues in the recent years. In this study, we evaluated the possibility of isolation of stem cells from oral connective tissue and investigated their characteristics.In this experimental study, sampling from the bone marrow and oral connective tissue of a beagle dog was performed under general anesthesia. Bone marrow stem cell isolation was performed according to the established protocols. The samples obtained from oral soft tissue were broken to small pieces and after adding collagenase I, the samples were incubated for 45 minutes in 37°C. Other processes were similar to the processes which were carried out on bone marrow cells. Then cell properties were compared to evaluate if the cells from the connective tissue were stem cells.The cells from the bone marrow and connective tissue had the same morphology. The result of colony forming unit assay was relatively similar. Population doubling time was similar too. In addition, both cell groups differentiated to osteoblasts in osteogenic media.The cells isolated from the oral connective tissue had the characteristics of stem cells, including fibroblastoid morphology, self renewal properties, high proliferation rate and differentiation potential.https://jdt.tums.ac.ir/index.php/jdt/article/view/792Connective TissueOralStem Cell |
| spellingShingle | P Torkzaban A Saffarpour M Bidgoli S Sohilifar In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells. Frontiers in Dentistry Connective Tissue Oral Stem Cell |
| title | In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells. |
| title_full | In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells. |
| title_fullStr | In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells. |
| title_full_unstemmed | In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells. |
| title_short | In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells. |
| title_sort | in vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells |
| topic | Connective Tissue Oral Stem Cell |
| url | https://jdt.tums.ac.ir/index.php/jdt/article/view/792 |
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