Arbuscular Mycorrhiza in Highly Fertilized Maize Cultures Alleviates Short-Term Drought Effects but Does Not Improve Fodder Yield and Quality
Under fertilization levels specific to intensive farming, the impact of compensation of soil nutritional value by arbuscular mycorrhiza (AM) might be limited. Therefore, the question arises whether modern crop varieties, selected for high NPK assimilation rate, are able to gain symbiotic benefits un...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-04-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00496/full |
| _version_ | 1818350756559847424 |
|---|---|
| author | Władysław Polcyn Ewelina Paluch-Lubawa Teresa Lehmann Robert Mikuła |
| author_facet | Władysław Polcyn Ewelina Paluch-Lubawa Teresa Lehmann Robert Mikuła |
| author_sort | Władysław Polcyn |
| collection | DOAJ |
| description | Under fertilization levels specific to intensive farming, the impact of compensation of soil nutritional value by arbuscular mycorrhiza (AM) might be limited. Therefore, the question arises whether modern crop varieties, selected for high NPK assimilation rate, are able to gain symbiotic benefits under other challenging field conditions, such as drought. Accordingly, in this study we aimed to evaluate the contribution of Rhizophagus irregularis to the drought response of a stay-green corn hybrid in pot cultures equally fertilized until silking, compared to non-mycorrhizal (NM) counterparts. The highest tested fertilization regime not detrimental to the long-term vitality of intraradical hyphae reached the levels recommended for field cultivation of silage corn, except phosphorus application restricted to 60%. Under normal watering, mycorrhiza increased leaf nitrogen and phosphorus acquisition but only in cultures supplied with low NPK levels. At high fertilization levels, only the older leaves retained AM dependency, whereas for other leaf positions the AM-NM differences were leveled out. The similar size and nutritional status of highly fertilized AM and NM cultures, used in this study, eliminated fungal benefits before and during the 2-week drought progression. Nevertheless, mycorrhizal contribution became evident at the time of renewed watering, when AM plants showed much faster reversal of drought-induced leaf senescence symptoms: impaired photosynthesis and nitrogen management. Our results suggest that mycorrhiza can alter drought-induced senescence even in stay-green mutants. Moreover, this effect was apparently not mediated by AM-improved growth but triggered by activation of fungal transport at the time of recovery. Interestingly, the fungal protective potential was shown to be preserved at the expense of lowering AM vesicle number. It can be interpreted as engagement of hyphal nutritional resources targeted to maintain the symbiotic relationship despite the reduced vitality of the host. Finally, we compared the productivity of AM and NM cultures subjected to short-term drought at silking time and further fertilized with moderate or high NPK doses until the grain-filling stage. The yield and nutritive value of green forage showed that alleviation of drought-induced senescence by AM was not sufficient to have a significant positive effect on the final productivity compared to NM plants. |
| first_indexed | 2024-12-13T18:26:54Z |
| format | Article |
| id | doaj.art-f47b95be6216480790ecf4ef36f309a4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T18:26:54Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f47b95be6216480790ecf4ef36f309a42022-12-21T23:35:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-04-011010.3389/fpls.2019.00496452422Arbuscular Mycorrhiza in Highly Fertilized Maize Cultures Alleviates Short-Term Drought Effects but Does Not Improve Fodder Yield and QualityWładysław Polcyn0Ewelina Paluch-Lubawa1Teresa Lehmann2Robert Mikuła3Department of Plant Physiology, Faculty of Horticulture and Landscape Architecture, Adam Mickiewicz University, Poznań, PolandDepartment of Plant Physiology, Faculty of Horticulture and Landscape Architecture, Adam Mickiewicz University, Poznań, PolandDepartment of Plant Physiology, Faculty of Horticulture and Landscape Architecture, Adam Mickiewicz University, Poznań, PolandDepartment of Animal Nutrition, Faculty of Veterinary Medicine and Animal Science, Poznań University of Life Sciences, Poznań, PolandUnder fertilization levels specific to intensive farming, the impact of compensation of soil nutritional value by arbuscular mycorrhiza (AM) might be limited. Therefore, the question arises whether modern crop varieties, selected for high NPK assimilation rate, are able to gain symbiotic benefits under other challenging field conditions, such as drought. Accordingly, in this study we aimed to evaluate the contribution of Rhizophagus irregularis to the drought response of a stay-green corn hybrid in pot cultures equally fertilized until silking, compared to non-mycorrhizal (NM) counterparts. The highest tested fertilization regime not detrimental to the long-term vitality of intraradical hyphae reached the levels recommended for field cultivation of silage corn, except phosphorus application restricted to 60%. Under normal watering, mycorrhiza increased leaf nitrogen and phosphorus acquisition but only in cultures supplied with low NPK levels. At high fertilization levels, only the older leaves retained AM dependency, whereas for other leaf positions the AM-NM differences were leveled out. The similar size and nutritional status of highly fertilized AM and NM cultures, used in this study, eliminated fungal benefits before and during the 2-week drought progression. Nevertheless, mycorrhizal contribution became evident at the time of renewed watering, when AM plants showed much faster reversal of drought-induced leaf senescence symptoms: impaired photosynthesis and nitrogen management. Our results suggest that mycorrhiza can alter drought-induced senescence even in stay-green mutants. Moreover, this effect was apparently not mediated by AM-improved growth but triggered by activation of fungal transport at the time of recovery. Interestingly, the fungal protective potential was shown to be preserved at the expense of lowering AM vesicle number. It can be interpreted as engagement of hyphal nutritional resources targeted to maintain the symbiotic relationship despite the reduced vitality of the host. Finally, we compared the productivity of AM and NM cultures subjected to short-term drought at silking time and further fertilized with moderate or high NPK doses until the grain-filling stage. The yield and nutritive value of green forage showed that alleviation of drought-induced senescence by AM was not sufficient to have a significant positive effect on the final productivity compared to NM plants.https://www.frontiersin.org/article/10.3389/fpls.2019.00496/fullarbuscular mycorrhizadrought tolerancefertilization limitsZea maysRhizophagus irregularissenescence reversal |
| spellingShingle | Władysław Polcyn Ewelina Paluch-Lubawa Teresa Lehmann Robert Mikuła Arbuscular Mycorrhiza in Highly Fertilized Maize Cultures Alleviates Short-Term Drought Effects but Does Not Improve Fodder Yield and Quality Frontiers in Plant Science arbuscular mycorrhiza drought tolerance fertilization limits Zea mays Rhizophagus irregularis senescence reversal |
| title | Arbuscular Mycorrhiza in Highly Fertilized Maize Cultures Alleviates Short-Term Drought Effects but Does Not Improve Fodder Yield and Quality |
| title_full | Arbuscular Mycorrhiza in Highly Fertilized Maize Cultures Alleviates Short-Term Drought Effects but Does Not Improve Fodder Yield and Quality |
| title_fullStr | Arbuscular Mycorrhiza in Highly Fertilized Maize Cultures Alleviates Short-Term Drought Effects but Does Not Improve Fodder Yield and Quality |
| title_full_unstemmed | Arbuscular Mycorrhiza in Highly Fertilized Maize Cultures Alleviates Short-Term Drought Effects but Does Not Improve Fodder Yield and Quality |
| title_short | Arbuscular Mycorrhiza in Highly Fertilized Maize Cultures Alleviates Short-Term Drought Effects but Does Not Improve Fodder Yield and Quality |
| title_sort | arbuscular mycorrhiza in highly fertilized maize cultures alleviates short term drought effects but does not improve fodder yield and quality |
| topic | arbuscular mycorrhiza drought tolerance fertilization limits Zea mays Rhizophagus irregularis senescence reversal |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00496/full |
| work_keys_str_mv | AT władysławpolcyn arbuscularmycorrhizainhighlyfertilizedmaizeculturesalleviatesshorttermdroughteffectsbutdoesnotimprovefodderyieldandquality AT ewelinapaluchlubawa arbuscularmycorrhizainhighlyfertilizedmaizeculturesalleviatesshorttermdroughteffectsbutdoesnotimprovefodderyieldandquality AT teresalehmann arbuscularmycorrhizainhighlyfertilizedmaizeculturesalleviatesshorttermdroughteffectsbutdoesnotimprovefodderyieldandquality AT robertmikuła arbuscularmycorrhizainhighlyfertilizedmaizeculturesalleviatesshorttermdroughteffectsbutdoesnotimprovefodderyieldandquality |