Trypanosoma evansi in dogs from Barão de Melgaço, Mato Grosso: Molecular prevalence
ABSTRACT: Trypanosoma evansi, the hemoflagellate pathogen that causes “surra,” is a protozoan that infects the widest variety of mammals worldwide, primarily horses, camels, dogs, and wild animals. Cases of canine trypanosomiasis caused by T.evansi have been reported in the Brazilian pantonal region...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2024-04-01
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000900501&lng=en&tlng=en |
| _version_ | 1797205773851295744 |
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| author | Ana Lúcia Vasconcelos Melissa Harumi Sumiyoshi Arlyson Sousa Ferreira Valéria Régia Franco Sousa Valéria Dutra Luciano Nakazato Eveline da Cruz Boa Sorte Ayres Arleana do Bom Parto Ferreira de Almeida |
| author_facet | Ana Lúcia Vasconcelos Melissa Harumi Sumiyoshi Arlyson Sousa Ferreira Valéria Régia Franco Sousa Valéria Dutra Luciano Nakazato Eveline da Cruz Boa Sorte Ayres Arleana do Bom Parto Ferreira de Almeida |
| author_sort | Ana Lúcia Vasconcelos |
| collection | DOAJ |
| description | ABSTRACT: Trypanosoma evansi, the hemoflagellate pathogen that causes “surra,” is a protozoan that infects the widest variety of mammals worldwide, primarily horses, camels, dogs, and wild animals. Cases of canine trypanosomiasis caused by T.evansi have been reported in the Brazilian pantonal region. This study determined the prevalence of T.evansi and investigate risk factors. The study used blood samples from dogs in the municipality of Barão de Melgaço in the pantonal of the state of Mato Grosso, Brazil. The owner was given a structured epidemiological questionnaire containing information about the risk factors. The quantitative polymerase chain reaction technique was used to detect T.evansi DNA using species-specific oligonucleotides TevF and TevR of the gene encoding a 227-bp portion of the glycoprotein of the variant surface (VSG) T.evansi Rode Trypanozoon (RoTat) 1.2. Two (prevalence of 0.5%) genetic materials of T.evansi were reported in the403 samples. Although, no risk factor was associated with infection (P > 0.05), proximity to vegetation and streams, as well as the presence of tabanids, rodents, and marsupials, which are factors in the occurrence of infection, were observed in positive dogs, implying that the municipality of Barão de Melgaço has epidemiological characteristics that allow canine infection by T.evansi. |
| first_indexed | 2024-04-24T08:56:27Z |
| format | Article |
| id | doaj.art-f47bb97c68ca41ac8a198a6ffa926362 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-04-24T08:56:27Z |
| publishDate | 2024-04-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-f47bb97c68ca41ac8a198a6ffa9263622024-04-16T07:39:31ZengUniversidade Federal de Santa MariaCiência Rural1678-45962024-04-0154910.1590/0103-8478cr20230335Trypanosoma evansi in dogs from Barão de Melgaço, Mato Grosso: Molecular prevalenceAna Lúcia Vasconceloshttps://orcid.org/0000-0003-1547-2652Melissa Harumi Sumiyoshihttps://orcid.org/0000-0001-7370-7611Arlyson Sousa Ferreirahttps://orcid.org/0000-0003-4385-5650Valéria Régia Franco Sousahttps://orcid.org/0000-0001-6209-4514Valéria Dutrahttps://orcid.org/0000-0002-6630-2293Luciano Nakazatohttps://orcid.org/0000-0002-1244-0690Eveline da Cruz Boa Sorte Ayreshttps://orcid.org/0000-0002-2831-5325Arleana do Bom Parto Ferreira de Almeidahttps://orcid.org/0000-0002-6694-1407ABSTRACT: Trypanosoma evansi, the hemoflagellate pathogen that causes “surra,” is a protozoan that infects the widest variety of mammals worldwide, primarily horses, camels, dogs, and wild animals. Cases of canine trypanosomiasis caused by T.evansi have been reported in the Brazilian pantonal region. This study determined the prevalence of T.evansi and investigate risk factors. The study used blood samples from dogs in the municipality of Barão de Melgaço in the pantonal of the state of Mato Grosso, Brazil. The owner was given a structured epidemiological questionnaire containing information about the risk factors. The quantitative polymerase chain reaction technique was used to detect T.evansi DNA using species-specific oligonucleotides TevF and TevR of the gene encoding a 227-bp portion of the glycoprotein of the variant surface (VSG) T.evansi Rode Trypanozoon (RoTat) 1.2. Two (prevalence of 0.5%) genetic materials of T.evansi were reported in the403 samples. Although, no risk factor was associated with infection (P > 0.05), proximity to vegetation and streams, as well as the presence of tabanids, rodents, and marsupials, which are factors in the occurrence of infection, were observed in positive dogs, implying that the municipality of Barão de Melgaço has epidemiological characteristics that allow canine infection by T.evansi.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000900501&lng=en&tlng=encanine trypanosomiasisPantanalprotozoaqPCR |
| spellingShingle | Ana Lúcia Vasconcelos Melissa Harumi Sumiyoshi Arlyson Sousa Ferreira Valéria Régia Franco Sousa Valéria Dutra Luciano Nakazato Eveline da Cruz Boa Sorte Ayres Arleana do Bom Parto Ferreira de Almeida Trypanosoma evansi in dogs from Barão de Melgaço, Mato Grosso: Molecular prevalence Ciência Rural canine trypanosomiasis Pantanal protozoa qPCR |
| title | Trypanosoma evansi in dogs from Barão de Melgaço, Mato Grosso: Molecular prevalence |
| title_full | Trypanosoma evansi in dogs from Barão de Melgaço, Mato Grosso: Molecular prevalence |
| title_fullStr | Trypanosoma evansi in dogs from Barão de Melgaço, Mato Grosso: Molecular prevalence |
| title_full_unstemmed | Trypanosoma evansi in dogs from Barão de Melgaço, Mato Grosso: Molecular prevalence |
| title_short | Trypanosoma evansi in dogs from Barão de Melgaço, Mato Grosso: Molecular prevalence |
| title_sort | trypanosoma evansi in dogs from barao de melgaco mato grosso molecular prevalence |
| topic | canine trypanosomiasis Pantanal protozoa qPCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000900501&lng=en&tlng=en |
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