Endometrial microbiota composition is associated with reproductive outcome in infertile patients
Abstract Background Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproduct...
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| Format: | Article |
| Language: | English |
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BMC
2022-01-01
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| Series: | Microbiome |
| Online Access: | https://doi.org/10.1186/s40168-021-01184-w |
| _version_ | 1798033471318261760 |
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| author | Inmaculada Moreno Iolanda Garcia-Grau David Perez-Villaroya Marta Gonzalez-Monfort Mustafa Bahçeci Marcelo J. Barrionuevo Sagiri Taguchi Elena Puente Michael Dimattina Mei Wei Lim Georgina Meneghini Mira Aubuchon Mark Leondires Alexandra Izquierdo Martina Perez-Olgiati Alejandro Chavez Ken Seethram Davide Bau Carlos Gomez Diana Valbuena Felipe Vilella Carlos Simon |
| author_facet | Inmaculada Moreno Iolanda Garcia-Grau David Perez-Villaroya Marta Gonzalez-Monfort Mustafa Bahçeci Marcelo J. Barrionuevo Sagiri Taguchi Elena Puente Michael Dimattina Mei Wei Lim Georgina Meneghini Mira Aubuchon Mark Leondires Alexandra Izquierdo Martina Perez-Olgiati Alejandro Chavez Ken Seethram Davide Bau Carlos Gomez Diana Valbuena Felipe Vilella Carlos Simon |
| author_sort | Inmaculada Moreno |
| collection | DOAJ |
| description | Abstract Background Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy. Methods Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments. Results A dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes. Conclusions Our findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies. Video Abstract. |
| first_indexed | 2024-04-11T20:30:59Z |
| format | Article |
| id | doaj.art-f483aeb848054e3faf4bd9c83375c623 |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-04-11T20:30:59Z |
| publishDate | 2022-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-f483aeb848054e3faf4bd9c83375c6232022-12-22T04:04:31ZengBMCMicrobiome2049-26182022-01-0110111710.1186/s40168-021-01184-wEndometrial microbiota composition is associated with reproductive outcome in infertile patientsInmaculada Moreno0Iolanda Garcia-Grau1David Perez-Villaroya2Marta Gonzalez-Monfort3Mustafa Bahçeci4Marcelo J. Barrionuevo5Sagiri Taguchi6Elena Puente7Michael Dimattina8Mei Wei Lim9Georgina Meneghini10Mira Aubuchon11Mark Leondires12Alexandra Izquierdo13Martina Perez-Olgiati14Alejandro Chavez15Ken Seethram16Davide Bau17Carlos Gomez18Diana Valbuena19Felipe Vilella20Carlos Simon21Igenomix Foundation, INCLIVA Health Research InstituteIgenomix Foundation, INCLIVA Health Research InstituteIgenomix R&DIgenomix Foundation, INCLIVA Health Research InstituteBahçeci GroupIVF FloridaOak Clinic SumiyoshiClinica FertiaDominion FertilityAlpha IVF and Women’s Specialists CentreGestanza Medicina ReproductivaMissouri Center for Reproductive MedicineRMA ConnecticutProcreaTecPregna Medicina ReproductivaNew Hope Fertility CenterPacific Centre for Reproductive MedicineIgenomix R&DIgenomix R&DIgenomix R&DIgenomix Foundation, INCLIVA Health Research InstituteIgenomix Foundation, INCLIVA Health Research InstituteAbstract Background Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy. Methods Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments. Results A dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes. Conclusions Our findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies. Video Abstract.https://doi.org/10.1186/s40168-021-01184-w |
| spellingShingle | Inmaculada Moreno Iolanda Garcia-Grau David Perez-Villaroya Marta Gonzalez-Monfort Mustafa Bahçeci Marcelo J. Barrionuevo Sagiri Taguchi Elena Puente Michael Dimattina Mei Wei Lim Georgina Meneghini Mira Aubuchon Mark Leondires Alexandra Izquierdo Martina Perez-Olgiati Alejandro Chavez Ken Seethram Davide Bau Carlos Gomez Diana Valbuena Felipe Vilella Carlos Simon Endometrial microbiota composition is associated with reproductive outcome in infertile patients Microbiome |
| title | Endometrial microbiota composition is associated with reproductive outcome in infertile patients |
| title_full | Endometrial microbiota composition is associated with reproductive outcome in infertile patients |
| title_fullStr | Endometrial microbiota composition is associated with reproductive outcome in infertile patients |
| title_full_unstemmed | Endometrial microbiota composition is associated with reproductive outcome in infertile patients |
| title_short | Endometrial microbiota composition is associated with reproductive outcome in infertile patients |
| title_sort | endometrial microbiota composition is associated with reproductive outcome in infertile patients |
| url | https://doi.org/10.1186/s40168-021-01184-w |
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