Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand
A substantial number of genetically encoded fluorescent sensors rely on the changes in FRET efficiency between fluorescent cores, measured in ratiometric mode, with acceptor photobleaching or by changes in fluorescence lifetime. We report on a modulated FRET acceptor allowing for simplified one-chan...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/8/4396 |
| _version_ | 1797410733118455808 |
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| author | Alexey S. Gavrikov Nina G. Bozhanova Mikhail S. Baranov Alexander S. Mishin |
| author_facet | Alexey S. Gavrikov Nina G. Bozhanova Mikhail S. Baranov Alexander S. Mishin |
| author_sort | Alexey S. Gavrikov |
| collection | DOAJ |
| description | A substantial number of genetically encoded fluorescent sensors rely on the changes in FRET efficiency between fluorescent cores, measured in ratiometric mode, with acceptor photobleaching or by changes in fluorescence lifetime. We report on a modulated FRET acceptor allowing for simplified one-channel FRET measurement based on a previously reported fluorogen-activating protein, DiB1. Upon the addition of the cell-permeable chromophore, the fluorescence of the donor-fluorescent protein mNeonGreen decreases, allowing for a simplified one-channel FRET measurement. The reported chemically modulated FRET acceptor is compatible with live-cell experiments and allows for prolonged time-lapse experiments with dynamic energy transfer evaluation. |
| first_indexed | 2024-03-09T04:34:28Z |
| format | Article |
| id | doaj.art-f488156ab3684a9982f8cf7ee4b495c5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:34:28Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f488156ab3684a9982f8cf7ee4b495c52023-12-03T13:30:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01238439610.3390/ijms23084396Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided LigandAlexey S. Gavrikov0Nina G. Bozhanova1Mikhail S. Baranov2Alexander S. Mishin3Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklay St. 16/10, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklay St. 16/10, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklay St. 16/10, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklay St. 16/10, 117997 Moscow, RussiaA substantial number of genetically encoded fluorescent sensors rely on the changes in FRET efficiency between fluorescent cores, measured in ratiometric mode, with acceptor photobleaching or by changes in fluorescence lifetime. We report on a modulated FRET acceptor allowing for simplified one-channel FRET measurement based on a previously reported fluorogen-activating protein, DiB1. Upon the addition of the cell-permeable chromophore, the fluorescence of the donor-fluorescent protein mNeonGreen decreases, allowing for a simplified one-channel FRET measurement. The reported chemically modulated FRET acceptor is compatible with live-cell experiments and allows for prolonged time-lapse experiments with dynamic energy transfer evaluation.https://www.mdpi.com/1422-0067/23/8/4396FRETfluorogen-activating proteinsFAPs |
| spellingShingle | Alexey S. Gavrikov Nina G. Bozhanova Mikhail S. Baranov Alexander S. Mishin Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand International Journal of Molecular Sciences FRET fluorogen-activating proteins FAPs |
| title | Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand |
| title_full | Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand |
| title_fullStr | Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand |
| title_full_unstemmed | Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand |
| title_short | Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand |
| title_sort | add and go fret acceptor for live cell measurements modulated by externally provided ligand |
| topic | FRET fluorogen-activating proteins FAPs |
| url | https://www.mdpi.com/1422-0067/23/8/4396 |
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