LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by <i>Amphidinium</i>
Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then qu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/10/497 |
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| author | Marvin Wellkamp Francisco García-Camacho Lorena M. Durán-Riveroll Jan Tebben Urban Tillmann Bernd Krock |
| author_facet | Marvin Wellkamp Francisco García-Camacho Lorena M. Durán-Riveroll Jan Tebben Urban Tillmann Bernd Krock |
| author_sort | Marvin Wellkamp |
| collection | DOAJ |
| description | Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM. |
| first_indexed | 2024-03-10T15:58:22Z |
| format | Article |
| id | doaj.art-f4894628b0774792bddd5382145e38a2 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T15:58:22Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-f4894628b0774792bddd5382145e38a22023-11-20T15:31:33ZengMDPI AGMarine Drugs1660-33972020-09-01181049710.3390/md18100497LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by <i>Amphidinium</i>Marvin Wellkamp0Francisco García-Camacho1Lorena M. Durán-Riveroll2Jan Tebben3Urban Tillmann4Bernd Krock5Alfred-Wegener-Institut für Polar und Meeresforschung, 27570 Bremerhaven, GermanyChemical Engineering Department, University of Almería, La Cañada, 04120 Almeria, SpainCONACyT-Departamento de Biotecnología Marina, Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada 22860, MexicoSection Ecological Chemistry, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, 27570 Bremerhaven, GermanySection Ecological Chemistry, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, 27570 Bremerhaven, GermanySection Ecological Chemistry, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, 27570 Bremerhaven, GermanyAmphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.https://www.mdpi.com/1660-3397/18/10/497phycotoxinichthyotoxindinoflagellateharmful algaeglycosylation |
| spellingShingle | Marvin Wellkamp Francisco García-Camacho Lorena M. Durán-Riveroll Jan Tebben Urban Tillmann Bernd Krock LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by <i>Amphidinium</i> Marine Drugs phycotoxin ichthyotoxin dinoflagellate harmful algae glycosylation |
| title | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by <i>Amphidinium</i> |
| title_full | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by <i>Amphidinium</i> |
| title_fullStr | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by <i>Amphidinium</i> |
| title_full_unstemmed | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by <i>Amphidinium</i> |
| title_short | LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by <i>Amphidinium</i> |
| title_sort | lc ms ms method development for the discovery and identification of amphidinols produced by i amphidinium i |
| topic | phycotoxin ichthyotoxin dinoflagellate harmful algae glycosylation |
| url | https://www.mdpi.com/1660-3397/18/10/497 |
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