Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain
Abstract Background Bacillus thuringiensis (Bt) is a widely used biopesticide. The bioinsecticide based on Bt is obtained by fermentation, but the substrates currently used for its production constitute ingredients of high commercial value. In this context, the use of agro-industrial residues as sub...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-01-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-023-00461-x |
| _version_ | 1827388242437079040 |
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| author | Hister M. M. dos Santos Camila de S. Varize Camilla A. S. Valença Fábio C. A. Dossi Marcus V. de Aragão Batista Roberta P. M. Fernandes Patricia Severino Eliana B. Souto Silvio S. Dolabella Marcelo da C. Mendonça Sona Jain |
| author_facet | Hister M. M. dos Santos Camila de S. Varize Camilla A. S. Valença Fábio C. A. Dossi Marcus V. de Aragão Batista Roberta P. M. Fernandes Patricia Severino Eliana B. Souto Silvio S. Dolabella Marcelo da C. Mendonça Sona Jain |
| author_sort | Hister M. M. dos Santos |
| collection | DOAJ |
| description | Abstract Background Bacillus thuringiensis (Bt) is a widely used biopesticide. The bioinsecticide based on Bt is obtained by fermentation, but the substrates currently used for its production constitute ingredients of high commercial value. In this context, the use of agro-industrial residues as substrates is an alternative to make the fermentation process viable on a large scale, in addition to minimizing environmental problems and contributing to the destination of these residues for biotechnological purposes. Results In the first part of this study, a previously isolated spore forming soil bacteria (Bv5) harboring and expressing a novel cry 8A gene was confirmed as B. thuringiensis based on its morphological characteristics, Gram staining, scanning electron microscopy (SEM) and genome sequencing. Bv5 was established as a Gram-positive spore forming bacteria with ellipsoidal spores and small round toxins. Bv5 genome comprised of the 5.30 Mb chromosome and two megaplasmids of 450 kb and 261 kb, respectively, with cry 8A gene located on the smallest megaplasmid. In the second part of the study, the physiological profile of the Bv5 strain during fermentation in different agro-industrial biowastes (cassava wastewater, orange pulp wash and whey) was analyzed. The fermentation experiment was divided into two stages. In the first stage, the agro-industrial waste with or without salts with the best results for biomass, spores and proteins production was selected. In the second stage, the effect of the selected medium in original and diluted form with the C:N balance was evaluated, in two different fermentation times (72 h and 96 h). Pulp wash enriched with salts was selected as the most suitable medium for the growth of Bv5 strain in the first stage. In the second stage pulp wash (without dilution) with the addition of salts, and with nitrogen supplementation, was considered the best for cell growth, spore and toxin production by Bv5. Conclusions To conclude, our study provide a new alternative for bio-waste from the orange juice industry, as well as potential culture medium for the Bt commercial scale production. |
| first_indexed | 2024-03-08T16:15:50Z |
| format | Article |
| id | doaj.art-f489c43690ec4fadad2d53f9e4e137f2 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-03-08T16:15:50Z |
| publishDate | 2024-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-f489c43690ec4fadad2d53f9e4e137f22024-01-07T12:39:28ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432024-01-0113111010.1186/s43088-023-00461-xUse of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strainHister M. M. dos Santos0Camila de S. Varize1Camilla A. S. Valença2Fábio C. A. Dossi3Marcus V. de Aragão Batista4Roberta P. M. Fernandes5Patricia Severino6Eliana B. Souto7Silvio S. Dolabella8Marcelo da C. Mendonça9Sona Jain10Industrial Biotechnology Program, University of Tiradentes (UNIT)Laboratório de Controle Biotecnológico de Pagas (LCBiotec), Empresa de Desenvolvimento Agropecuário Do Estado de Sergipe (EMDAGRO)Industrial Biotechnology Program, University of Tiradentes (UNIT)Laboratory of Colloidal Systems Studies, Institute of Technology and ResearchDepartment of Biology, Federal University of SergipeDepartment of Physiology, Federal University of SergipeIndustrial Biotechnology Program, University of Tiradentes (UNIT)UCIBIO – Applied Molecular Biosciences Unit, MEDTECH, Laboratory of Pharmaceutical Technology, Department of Drug Sciences, Faculty of Pharmacy, University of PortoDepartment of Morphology, Federal University of SergipeIndustrial Biotechnology Program, University of Tiradentes (UNIT)Industrial Biotechnology Program, University of Tiradentes (UNIT)Abstract Background Bacillus thuringiensis (Bt) is a widely used biopesticide. The bioinsecticide based on Bt is obtained by fermentation, but the substrates currently used for its production constitute ingredients of high commercial value. In this context, the use of agro-industrial residues as substrates is an alternative to make the fermentation process viable on a large scale, in addition to minimizing environmental problems and contributing to the destination of these residues for biotechnological purposes. Results In the first part of this study, a previously isolated spore forming soil bacteria (Bv5) harboring and expressing a novel cry 8A gene was confirmed as B. thuringiensis based on its morphological characteristics, Gram staining, scanning electron microscopy (SEM) and genome sequencing. Bv5 was established as a Gram-positive spore forming bacteria with ellipsoidal spores and small round toxins. Bv5 genome comprised of the 5.30 Mb chromosome and two megaplasmids of 450 kb and 261 kb, respectively, with cry 8A gene located on the smallest megaplasmid. In the second part of the study, the physiological profile of the Bv5 strain during fermentation in different agro-industrial biowastes (cassava wastewater, orange pulp wash and whey) was analyzed. The fermentation experiment was divided into two stages. In the first stage, the agro-industrial waste with or without salts with the best results for biomass, spores and proteins production was selected. In the second stage, the effect of the selected medium in original and diluted form with the C:N balance was evaluated, in two different fermentation times (72 h and 96 h). Pulp wash enriched with salts was selected as the most suitable medium for the growth of Bv5 strain in the first stage. In the second stage pulp wash (without dilution) with the addition of salts, and with nitrogen supplementation, was considered the best for cell growth, spore and toxin production by Bv5. Conclusions To conclude, our study provide a new alternative for bio-waste from the orange juice industry, as well as potential culture medium for the Bt commercial scale production.https://doi.org/10.1186/s43088-023-00461-xBacillus thuringiensisBiopesticidesBio-wasteCry geneFermentation |
| spellingShingle | Hister M. M. dos Santos Camila de S. Varize Camilla A. S. Valença Fábio C. A. Dossi Marcus V. de Aragão Batista Roberta P. M. Fernandes Patricia Severino Eliana B. Souto Silvio S. Dolabella Marcelo da C. Mendonça Sona Jain Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain Beni-Suef University Journal of Basic and Applied Sciences Bacillus thuringiensis Biopesticides Bio-waste Cry gene Fermentation |
| title | Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain |
| title_full | Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain |
| title_fullStr | Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain |
| title_full_unstemmed | Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain |
| title_short | Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain |
| title_sort | use of agro industrial bio waste for the growth and production of a previously isolated bacillus thuringiensis strain |
| topic | Bacillus thuringiensis Biopesticides Bio-waste Cry gene Fermentation |
| url | https://doi.org/10.1186/s43088-023-00461-x |
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