Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene delivery
Abstract Multicomponent nanoparticle systems are known for their varied properties and functions, and have shown potential as gene nanocarriers. This study aims to synthesize and characterize ternary nickel–cobalt-ferrite (NiCoFe2O4) nanoparticles with the potential to serve as gene nanocarriers for...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Journal of Biological Engineering |
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| Online Access: | https://doi.org/10.1186/s13036-023-00381-5 |
| _version_ | 1797559147040866304 |
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| author | Hajar Q. Alijani Mehrdad Khatami Masoud Torkzadeh-Mahani Jan Michalička Wu Wang Di Wang Abolfazl Heydari |
| author_facet | Hajar Q. Alijani Mehrdad Khatami Masoud Torkzadeh-Mahani Jan Michalička Wu Wang Di Wang Abolfazl Heydari |
| author_sort | Hajar Q. Alijani |
| collection | DOAJ |
| description | Abstract Multicomponent nanoparticle systems are known for their varied properties and functions, and have shown potential as gene nanocarriers. This study aims to synthesize and characterize ternary nickel–cobalt-ferrite (NiCoFe2O4) nanoparticles with the potential to serve as gene nanocarriers for cancer/gene therapy. The biogenic nanocarriers were prepared using a simple and eco-friendly method following green chemistry principles. The physicochemical properties of the nanoparticles were analyzed by X-ray diffraction, vibrating sample magnetometer, X-ray photoelectron spectroscopy, and Brunauer–Emmett–Teller. To evaluate the morphology of the nanoparticles, the field emission scanning electron microscopy with energy dispersive X-Ray spectroscopy, high-resolution transmission electron microscopy imaging, and electron tomography were conducted. Results indicate the nanoparticles have a nanoflower morphology with a mesoporous nature and a cubic spinel structure, where the rod and spherical nanoparticles became rose-like with a specific orientation. These nanoparticles were found to have minimal toxicity in human embryonic kidney 293 (HEK-293 T) cells at concentrations of 1 to 250 µg·mL–1. We also demonstrated that the nanoparticles could be used as gene nanocarriers for delivering genes to HEK-293 T cells using an external magnetic field, with optimal transfection efficiency achieved at an N/P ratio of 2.5. The study suggests that biogenic multicomponent nanocarriers show potential for safe and efficient gene delivery in cancer/gene therapy. Graphical Abstract |
| first_indexed | 2024-03-10T17:41:19Z |
| format | Article |
| id | doaj.art-f48b57289bed448782d1c333d2e5032c |
| institution | Directory Open Access Journal |
| issn | 1754-1611 |
| language | English |
| last_indexed | 2024-03-10T17:41:19Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj.art-f48b57289bed448782d1c333d2e5032c2023-11-20T09:41:35ZengBMCJournal of Biological Engineering1754-16112023-10-0117111910.1186/s13036-023-00381-5Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene deliveryHajar Q. Alijani0Mehrdad Khatami1Masoud Torkzadeh-Mahani2Jan Michalička3Wu Wang4Di Wang5Abolfazl Heydari6Department of Biotechnology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced TechnologyDepartment of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares, UniversityDepartment of Biotechnology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced TechnologyCentral European Institute of Technology, Brno University of TechnologyInstitute of Nanotechnology, Karlsruhe Institute of TechnologyKarlsruhe Nano Micro Facility, Karlsruhe Institute of TechnologyPolymer Institute of the Slovak Academy of ScienceAbstract Multicomponent nanoparticle systems are known for their varied properties and functions, and have shown potential as gene nanocarriers. This study aims to synthesize and characterize ternary nickel–cobalt-ferrite (NiCoFe2O4) nanoparticles with the potential to serve as gene nanocarriers for cancer/gene therapy. The biogenic nanocarriers were prepared using a simple and eco-friendly method following green chemistry principles. The physicochemical properties of the nanoparticles were analyzed by X-ray diffraction, vibrating sample magnetometer, X-ray photoelectron spectroscopy, and Brunauer–Emmett–Teller. To evaluate the morphology of the nanoparticles, the field emission scanning electron microscopy with energy dispersive X-Ray spectroscopy, high-resolution transmission electron microscopy imaging, and electron tomography were conducted. Results indicate the nanoparticles have a nanoflower morphology with a mesoporous nature and a cubic spinel structure, where the rod and spherical nanoparticles became rose-like with a specific orientation. These nanoparticles were found to have minimal toxicity in human embryonic kidney 293 (HEK-293 T) cells at concentrations of 1 to 250 µg·mL–1. We also demonstrated that the nanoparticles could be used as gene nanocarriers for delivering genes to HEK-293 T cells using an external magnetic field, with optimal transfection efficiency achieved at an N/P ratio of 2.5. The study suggests that biogenic multicomponent nanocarriers show potential for safe and efficient gene delivery in cancer/gene therapy. Graphical Abstracthttps://doi.org/10.1186/s13036-023-00381-5Ternary metal nanoflowersGreen Chemistry3D electron tomographyMesoporous biogenic nanocarriersGene delivery |
| spellingShingle | Hajar Q. Alijani Mehrdad Khatami Masoud Torkzadeh-Mahani Jan Michalička Wu Wang Di Wang Abolfazl Heydari Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene delivery Journal of Biological Engineering Ternary metal nanoflowers Green Chemistry 3D electron tomography Mesoporous biogenic nanocarriers Gene delivery |
| title | Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene delivery |
| title_full | Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene delivery |
| title_fullStr | Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene delivery |
| title_full_unstemmed | Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene delivery |
| title_short | Biosynthesis of ternary NiCoFe2O4 nanoflowers: investigating their 3D structure and potential use in gene delivery |
| title_sort | biosynthesis of ternary nicofe2o4 nanoflowers investigating their 3d structure and potential use in gene delivery |
| topic | Ternary metal nanoflowers Green Chemistry 3D electron tomography Mesoporous biogenic nanocarriers Gene delivery |
| url | https://doi.org/10.1186/s13036-023-00381-5 |
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