Production of L-Asparaginase (L-ASN) from endophytic Lasiodiplodia theobromae hosted Teucrium polium in Egypt
Endophytic fungi produce important bioactive primary and secondary metabolites. Researchers used L-Asparaginase for the treatment of Acute Lymphoblastic leukemia (ALL). Endophytic taxon, <em>Lasiodiplodia theobromae </em>(<em>Pat</em>.) Griff. and Maubl. has been isolated fro...
| Format: | Article |
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| Language: | English |
| Published: |
Arab Society for Fungal Conservation
2018-12-01
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| Series: | Microbial Biosystems Journal |
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| Online Access: | https://mb.journals.ekb.eg/article_26276_6ccb25d00e3c2d0317f7aec619ee5115.pdf |
| _version_ | 1818760112658972672 |
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| collection | DOAJ |
| description | Endophytic fungi produce important bioactive primary and secondary metabolites. Researchers used L-Asparaginase for the treatment of Acute Lymphoblastic leukemia (ALL). Endophytic taxon, <em>Lasiodiplodia theobromae </em>(<em>Pat</em>.) Griff. and Maubl. has been isolated from <em>Teucrium polium</em> plant tissues collected from Saint Katherine Protectorate and was screened for its ability to produce L-Asparaginase qualitatively and quantitatively. The crude enzyme has been partially purified using gel-filtration Sephadex G-100 with an activity of 21.00 lMmL<sup>-1</sup> min<sup>-1</sup>. The results revealed that <em>L</em>. <em>theobromae</em> (SCUF-TP2016) represents a rich source of bioactive metabolites that can be manipulated to produce L-Asparaginase which is a desirable novel analog for chemotherapy. |
| first_indexed | 2024-12-18T06:53:27Z |
| format | Article |
| id | doaj.art-f48fae08d6fd43d1b8d605ae0cdd0b51 |
| institution | Directory Open Access Journal |
| issn | 2357-0326 2357-0334 |
| language | English |
| last_indexed | 2024-12-18T06:53:27Z |
| publishDate | 2018-12-01 |
| publisher | Arab Society for Fungal Conservation |
| record_format | Article |
| series | Microbial Biosystems Journal |
| spelling | doaj.art-f48fae08d6fd43d1b8d605ae0cdd0b512022-12-21T21:17:15ZengArab Society for Fungal ConservationMicrobial Biosystems Journal2357-03262357-03342018-12-0132465510.21608/mb.2018.2627626276Production of L-Asparaginase (L-ASN) from endophytic Lasiodiplodia theobromae hosted Teucrium polium in EgyptEndophytic fungi produce important bioactive primary and secondary metabolites. Researchers used L-Asparaginase for the treatment of Acute Lymphoblastic leukemia (ALL). Endophytic taxon, <em>Lasiodiplodia theobromae </em>(<em>Pat</em>.) Griff. and Maubl. has been isolated from <em>Teucrium polium</em> plant tissues collected from Saint Katherine Protectorate and was screened for its ability to produce L-Asparaginase qualitatively and quantitatively. The crude enzyme has been partially purified using gel-filtration Sephadex G-100 with an activity of 21.00 lMmL<sup>-1</sup> min<sup>-1</sup>. The results revealed that <em>L</em>. <em>theobromae</em> (SCUF-TP2016) represents a rich source of bioactive metabolites that can be manipulated to produce L-Asparaginase which is a desirable novel analog for chemotherapy.https://mb.journals.ekb.eg/article_26276_6ccb25d00e3c2d0317f7aec619ee5115.pdfanti-leukemicenzymessaint katherine protectorategel filtration |
| spellingShingle | Production of L-Asparaginase (L-ASN) from endophytic Lasiodiplodia theobromae hosted Teucrium polium in Egypt Microbial Biosystems Journal anti-leukemic enzymes saint katherine protectorate gel filtration |
| title | Production of L-Asparaginase (L-ASN) from endophytic Lasiodiplodia theobromae hosted Teucrium polium in Egypt |
| title_full | Production of L-Asparaginase (L-ASN) from endophytic Lasiodiplodia theobromae hosted Teucrium polium in Egypt |
| title_fullStr | Production of L-Asparaginase (L-ASN) from endophytic Lasiodiplodia theobromae hosted Teucrium polium in Egypt |
| title_full_unstemmed | Production of L-Asparaginase (L-ASN) from endophytic Lasiodiplodia theobromae hosted Teucrium polium in Egypt |
| title_short | Production of L-Asparaginase (L-ASN) from endophytic Lasiodiplodia theobromae hosted Teucrium polium in Egypt |
| title_sort | production of l asparaginase l asn from endophytic lasiodiplodia theobromae hosted teucrium polium in egypt |
| topic | anti-leukemic enzymes saint katherine protectorate gel filtration |
| url | https://mb.journals.ekb.eg/article_26276_6ccb25d00e3c2d0317f7aec619ee5115.pdf |